CFAP97D1 (NM_001136483) Human Tagged ORF Clone

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SKU
RC227178
C17orf105 (Myc-DDK-tagged)-Human chromosome 17 open reading frame 105 (C17orf105)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CFAP97D1
Synonyms C17orf105
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227178 representing NM_001136483
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAATTCCCTGGATTATCTGGCCTACCCTGTTATCGTCTCTAATCACAGGCAAAGCACAACCTTCA
GAAAGAAACTGGACTTTGGCCACTACGTATCTCACAAGAATAGAATACAAATAGCGAAGCCTACTGTTGA
TACCAAACCTCCAGTGGCGCACACAAATCACATTTTAAAATTGAGCAAACTACAGGGTGAACAAAAGAAA
ATCAACAAAATCGAGTATGAAAACAAGCAACTGTGTCAGAAAATCGCAAATGCCCATCGCGGCCCTGCCA
AGGTGGATTGCTGGAATGAATATTTTTCCAAGAGCTTAAACAGAGAAACAAGGAACCGCGAGCTAGTGAG
AATCACCATGGAAAACCAGGGCATTCTGAAGAGGCTTGTTGATCGCAAACCCCACTATGACCGCAGGGCA
TCTGAGATAGACTGGCAGAATTCAAGGCGCTATATCAGAAATACCACGAGATATCTTCTCTCCCAAAATG
AA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC227178 representing NM_001136483
Red=Cloning site Green=Tags(s)

MNNSLDYLAYPVIVSNHRQSTTFRKKLDFGHYVSHKNRIQIAKPTVDTKPPVAHTNHILKLSKLQGEQKK
INKIEYENKQLCQKIANAHRGPAKVDCWNEYFSKSLNRETRNRELVRITMENQGILKRLVDRKPHYDRRA
SEIDWQNSRRYIRNTTRYLLSQNE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001136483
ORF Size 492 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001136483.3
RefSeq ORF 495 bp
Locus ID 284067
UniProt ID B2RV13
Cytogenetics 17q21.31
MW 19.4 kDa
Summary Required for male fertility through its role in axonemal doublet stabilization which is essential for sperm motility and fertilization.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001136483" in other vectors (4)
SKU Description Size Price
RC227178L3 Lenti ORF clone of Human chromosome 17 open reading frame 105 (C17orf105), Myc-DDK-tagged 10 ug
$450.00
RC227178L4 Lenti ORF clone of Human chromosome 17 open reading frame 105 (C17orf105), mGFP tagged 10 ug
$450.00
RG227178 C17orf105 (tGFP-tagged) - Human chromosome 17 open reading frame 105 (C17orf105) 10 ug
$350.00
SC325542 C17orf105 (untagged)-Human chromosome 17 open reading frame 105 (C17orf105) 10 ug
$165.00

Plasmid Map for RC227178

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Cloning scheme for RC227178

Circular map for RC227178

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