CHID1 (NM_001142675) Human Tagged ORF Clone

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SKU
RC226851
CHID1 (Myc-DDK-tagged)-Human chitinase domain containing 1 (CHID1), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CHID1
Synonyms GL008; SI-CLP; SICLP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226851 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGACACTCTTCAACCTCCTCTGGCTTGCCCTGGCCTGCAGCCCTGTTCACACTACCCTGTCAAAGT
CAGATGCCAAAAAAGCCGCCTCAAAGACGCTGCTGGAGAAGAGTCAGTTTTCAGATAAGCCGGTGCAAGA
CCGGGGTTTGGTGGTGACGGACCTCAAAGCTGAGAGTGTGGTTCTTGAGCATCGCAGCTACTGCTCGGCA
AAGGCCCGGGACAGACACTTTGCTGGGGATGTACTGGGCTATGTCACTCCATGGAACAGCCATGGCTACG
ATGTCACCAAGGTCTTTGGGAGCAAGTTCACACAGATCTCACCCGTCTGGCTGCAGCTGAAGAGACGTGG
CCGTGAGATGTTTGAGGTCACGGGCCTCCACGACGTGGACCAAGGGTGGATGCGAGCTGTCAGGAAGCAT
GCCAAGGGCCTGCACATAGTGCCTCGGCTCCTGTTTGAGGACTGGACTTACGATGATTTCCGGAACGTCT
TAGACAGTGAGGATGAGATAGAGGAGCTGAGCAAGACCGTGGTCCAGGTGGCAAAGAACCAGCATTTCGA
TGGCTTCGTGGTGGAGGTCTGGAACCAGCTGCTAAGCCAGAAGCGCGTGGGCCTCATCCACATGCTCACC
CACTTGGCCGAGGCTCTGCACCAGGCCCGGCTGCTGGCCCTCCTGGTCATCCCGCCTGCCATCACCCCCG
GGACCGACCAGCTGGGCATGTTCACGCACAAGGAGTTTGAGCAGCTGGCCCCCGTGCTGGATGGTTTCAG
CCTCATGACCTACGACTACTCTACAGCGCATCAGCCTGGCCCTAATGCACCCCTGTCCTGGGTTCGAGCC
TGCGTCCAGGTCCTGGACCCGAAGTCCAAGTGGCGAAGCAAAATCCTCCTGGGGCTCAACTTCTATGGTA
TGGACTACGCGACCTCCAAGGATGCCCGTGAGCCTGTTGTCGGGGCCAGGTACATCCAGACACTGAAGGA
CCACAGGCCCCGGATGGTGTGGGACAGCCAGGTCTCAGAGCACTTCTTCGAGTACAAGAAGAGCCGCAGT
GGGAGGCACGTCGTCTTCTACCCAACCCTGAAGTCCCTGCAGGTGCGGCTGGAGCTGGCCCGGGAGCTGG
GCGTTGGGGTCTCTATCTGGGAGCTGGGCCAGGGCCTGGACTACTTCTACGACCTGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC226851 protein sequence
Red=Cloning site Green=Tags(s)

MRTLFNLLWLALACSPVHTTLSKSDAKKAASKTLLEKSQFSDKPVQDRGLVVTDLKAESVVLEHRSYCSA
KARDRHFAGDVLGYVTPWNSHGYDVTKVFGSKFTQISPVWLQLKRRGREMFEVTGLHDVDQGWMRAVRKH
AKGLHIVPRLLFEDWTYDDFRNVLDSEDEIEELSKTVVQVAKNQHFDGFVVEVWNQLLSQKRVGLIHMLT
HLAEALHQARLLALLVIPPAITPGTDQLGMFTHKEFEQLAPVLDGFSLMTYDYSTAHQPGPNAPLSWVRA
CVQVLDPKSKWRSKILLGLNFYGMDYATSKDAREPVVGARYIQTLKDHRPRMVWDSQVSEHFFEYKKSRS
GRHVVFYPTLKSLQVRLELARELGVGVSIWELGQGLDYFYDLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001142675
ORF Size 1179 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001142675.1, NP_001136147.1
RefSeq Size 3558 bp
RefSeq ORF 1182 bp
Locus ID 66005
UniProt ID Q9BWS9
Cytogenetics 11p15.5
MW 45 kDa
Summary Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001142675" in other vectors (6)
SKU Description Size Price
RC226851L1 Lenti ORF clone of Human chitinase domain containing 1 (CHID1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC226851L2 Lenti ORF clone of Human chitinase domain containing 1 (CHID1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC226851L3 Lenti ORF clone of Human chitinase domain containing 1 (CHID1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC226851L4 Lenti ORF clone of Human chitinase domain containing 1 (CHID1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG226851 CHID1 (tGFP-tagged) - Human chitinase domain containing 1 (CHID1), transcript variant 1 10 ug
$657.00
SC325040 CHID1 (untagged)-Human chitinase domain containing 1 (CHID1), transcript variant 1 10 ug
$457.00

Plasmid Map for RC226851

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Cloning scheme for RC226851

Circular map for RC226851

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