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TMCC1 (NM_001128224) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC225924
TMCC1 (Myc-DDK-tagged)-Human transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $502.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMCC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC225924 representing NM_001128224
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAGAGGGACAAGCTTGCATAGTAGGCGGGGCAAGCCAGAGGCCCCAAAGGGAAGTCCCCAAATCA
ACAGGAAGTCTGGTCAGGAGATGACAGCTGTTATGCAGTCAGGCCGACCCAGGTCTTCATCCACAACTGA
TGCACCTACCAGCTCTGCTATGATGGAAATAGCTTGTGCTGCTGCTGCTGCTGCTGCTGCATGTCTACCA
GGAGAGGAGGGAACTGCGGAGCGGATCGAACGGTTGGAAGTAAGCAGCCTTGCCCAAACATCCAGTGCAG
TGGCCTCCAGTACCGATGGCAGCATCCACACAGACTCTGTGGATGGAACACCAGACCCTCAGCGCACAAA
GGCTGCCATTGCTCACCTGCAGCAGAAGATCCTGAAGCTCACAGAACAAATCAAGATTGCACAAACAGCC
CGGGACGACAACGTTGCTGAATACTTGAAGCTTGCCAACAGTGCAGACAAACAGCAGGCTGCCCGCATCA
AGCAAGTCTTTGAGAAGAAGAACCAGAAATCTGCCCAAACTATCCTCCAGCTGCAAAAGAAACTTGAGCA
CTACCACAGGAAGCTCAGAGAGGTAGAGCAGAATGGGATCCCCCGGCAGCCAAAGGATGTCTTCAGGGAC
ATGCACCAGGGTCTGAAGGATGTAGGAGCAAAGGTGACTGGCTTCAGTGAAGGTGTGGTGGATAGTGTCA
AAGGTGGGTTTTCCAGCTTCTCCCAGGCCACCCATTCAGCAGCAGGCGCTGTAGTCTCAAAGCCCAGAGA
GATTGCCTCACTCATTCGGAACAAATTTGGCAGTGCAGACAACATCCCCAACCTGAAGGACTCTTTAGAG
GAAGGGCAAGTGGATGATGCGGGGAAGGCTTTGGGAGTGATTTCAAACTTTCAGTCTAGCCCAAAATATG
GTAGTGAAGAAGATTGTTCTAGTGCCACTTCAGGCTCAGTGGGAGCCAACAGCACCACAGGGGGCATCGC
TGTAGGAGCATCCAGCTCCAAAACAAACACCCTGGACATGCAGAGCTCAGGATTTGATGCACTACTACAT
GAGATCCAGGAGATCCGGGAAACCCAGGCCAGACTAGAGGAATCCTTTGAGACTCTCAAGGAACATTATC
AGAGGGACTATTCCTTAATAATGCAGACCTTACAGGAGGAGCGATATAGATGTGAACGATTGGAAGAACA
GCTAAATGACCTAACAGAGCTCCACCAGAATGAAATCTTGAACTTGAAGCAGGAACTGGCAAGCATGGAA
GAAAAAATCGCGTATCAGTCCTATGAACGGGCCCGGGACATCCAGGAGGCCCTGGAGGCATGCCAGACGC
GCATCTCCAAGATGGAGCTGCAGCAGCAGCAGCAGCAGGTGGTGCAGCTAGAAGGGCTGGAGAATGCCAC
TGCCCGGAACCTTCTGGGCAAACTCATCAACATCCTCCTGGCTGTCATGGCAGTCCTTTTGGTCTTTGTC
TCCACTGTAGCCAACTGTGTGGTCCCCCTCATGAAGACTCGCAACAGGACGTTCAGCACTTTATTCCTTG
TGGTTTTTATTGCCTTTCTCTGGAAGCACTGGGACGCCCTCTTCAGCTATGTGGAACGGTTCTTTTCATC
CCCTAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC225924 representing NM_001128224
Red=Cloning site Green=Tags(s)

MKRGTSLHSRRGKPEAPKGSPQINRKSGQEMTAVMQSGRPRSSSTTDAPTSSAMMEIACAAAAAAAACLP
GEEGTAERIERLEVSSLAQTSSAVASSTDGSIHTDSVDGTPDPQRTKAAIAHLQQKILKLTEQIKIAQTA
RDDNVAEYLKLANSADKQQAARIKQVFEKKNQKSAQTILQLQKKLEHYHRKLREVEQNGIPRQPKDVFRD
MHQGLKDVGAKVTGFSEGVVDSVKGGFSSFSQATHSAAGAVVSKPREIASLIRNKFGSADNIPNLKDSLE
EGQVDDAGKALGVISNFQSSPKYGSEEDCSSATSGSVGANSTTGGIAVGASSSKTNTLDMQSSGFDALLH
EIQEIRETQARLEESFETLKEHYQRDYSLIMQTLQEERYRCERLEEQLNDLTELHQNEILNLKQELASME
EKIAYQSYERARDIQEALEACQTRISKMELQQQQQQVVQLEGLENATARNLLGKLINILLAVMAVLLVFV
STVANCVVPLMKTRNRTFSTLFLVVFIAFLWKHWDALFSYVERFFSSPR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001128224
ORF Size 1617 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001128224.2, NP_001121696.1
RefSeq ORF 1620 bp
Locus ID 23023
UniProt ID O94876
Cytogenetics 3q22.1
Protein Families Transmembrane
MW 59.2 kDa
Summary Endoplasmic reticulum membrane protein that promotes endoplasmic reticulum-associated endosome fission (PubMed:30220460). Localizes to contact sites between the endoplasmic reticulum and endosomes and acts by promoting recruitment of the endoplasmic reticulum to endosome tubules for fission (PubMed:30220460). Endosome membrane fission of early and late endosomes is essential to separate regions destined for lysosomal degradation from carriers to be recycled to the plasma membrane (PubMed:30220460).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001128224" in other vectors (4)
SKU Description Size Price
RC225924L3 Lenti-ORF clone of TMCC1 (Myc-DDK-tagged)-Human transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant 3 10 ug
$802.00
RC225924L4 Lenti-ORF clone of TMCC1 (mGFP-tagged)-Human transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant 3 10 ug
$802.00
RG225924 TMCC1 (tGFP-tagged) - Human transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant 3 10 ug
$702.00
SC323126 TMCC1 (untagged)-Human transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant 3 10 ug
$553.00

Plasmid Map for RC225924

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC225924
Circular map for RC225924

file-alt Citations

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