TMCC1 (NM_001128224) Human Untagged Clone

SKU SC323126

TMCC1 (untagged)-Human transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: TMCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC323126 representing NM_001128224.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAAGAGAGGGACAAGCTTGCATAGTAGGCGGGGCAAGCCAGAGGCCCCAAAGGGAAGTCCCCAAATC; AACAGGAAGTCTGGTCAGGAGATGACAGCTGTTATGCAGTCAGGCCGACCCAGGTCTTCATCCACAACT; GATGCACCTACCAGCTCTGCTATGATGGAAATAGCTTGTGCTGCTGCTGCTGCTGCTGCTGCATGTCTA; CCAGGAGAGGAGGGAACTGCGGAGCGGATCGAACGGTTGGAAGTAAGCAGCCTTGCCCAAACATCCAGT; GCAGTGGCCTCCAGTACCGATGGCAGCATCCACACAGACTCTGTGGATGGAACACCAGACCCTCAGCGC; ACAAAGGCTGCCATTGCTCACCTGCAGCAGAAGATCCTGAAGCTCACAGAACAAATCAAGATTGCACAA; ACAGCCCGGGACGACAACGTTGCTGAATACTTGAAGCTTGCCAACAGTGCAGACAAACAGCAGGCTGCC; CGCATCAAGCAAGTCTTTGAGAAGAAGAACCAGAAATCTGCCCAAACTATCCTCCAGCTGCAAAAGAAA; CTTGAGCACTACCACAGGAAGCTCAGAGAGGTAGAGCAGAATGGGATCCCCCGGCAGCCAAAGGATGTC; TTCAGGGACATGCACCAGGGTCTGAAGGATGTAGGAGCAAAGGTGACTGGCTTCAGTGAAGGTGTGGTG; GATAGTGTCAAAGGTGGGTTTTCCAGCTTCTCCCAGGCCACCCATTCAGCAGCAGGCGCTGTAGTCTCA; AAGCCCAGAGAGATTGCCTCACTCATTCGGAACAAATTTGGCAGTGCAGACAACATCCCCAACCTGAAG; GACTCTTTAGAGGAAGGGCAAGTGGATGATGCGGGGAAGGCTTTGGGAGTGATTTCAAACTTTCAGTCT; AGCCCAAAATATGGTAGTGAAGAAGATTGTTCTAGTGCCACTTCAGGCTCAGTGGGAGCCAACAGCACC; ACAGGGGGCATCGCTGTAGGAGCATCCAGCTCCAAAACAAACACCCTGGACATGCAGAGCTCAGGATTT; GATGCACTACTACATGAGATCCAGGAGATCCGGGAAACCCAGGCCAGACTAGAGGAATCCTTTGAGACT; CTCAAGGAACATTATCAGAGGGACTATTCCTTAATAATGCAGACCTTACAGGAGGAGCGATATAGATGT; GAACGATTGGAAGAACAGCTAAATGACCTAACAGAGCTCCACCAGAATGAAATCTTGAACTTGAAGCAG; GAACTGGCAAGCATGGAAGAAAAAATCGCGTATCAGTCCTATGAACGGGCCCGGGACATCCAGGAGGCC; CTGGAGGCATGCCAGACGCGCATCTCCAAGATGGAGCTGCAGCAGCAGCAGCAGCAGGTGGTGCAGCTA; GAAGGGCTGGAGAATGCCACTGCCCGGAACCTTCTGGGCAAACTCATCAACATCCTCCTGGCTGTCATG; GCAGTCCTTTTGGTCTTTGTCTCCACTGTAGCCAACTGTGTGGTCCCCCTCATGAAGACTCGCAACAGG; ACGTTCAGCACTTTATTCCTTGTGGTTTTTATTGCCTTTCTCTGGAAGCACTGGGACGCCCTCTTCAGC; TATGTGGAACGGTTCTTTTCATCCCCTAGATGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001128224
• Insert Size: 1620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001128224.2
• RefSeq Size: 5496 bp
• RefSeq ORF: 1620 bp
• Locus ID: 23023
• UniProt ID: O94876
• Cytogenetics: 3q22.1
• Protein Families: Transmembrane
• MW: 59.4 kDa
• Summary: Endoplasmic reticulum membrane protein that promotes endoplasmic reticulum-associated endosome fission (PubMed:30220460). Localizes to contact sites between the endoplasmic reticulum and endosomes and acts by promoting recruitment of the endoplasmic reticulum to endosome tubules for fission (PubMed:30220460). Endosome membrane fission of early and late endosomes is essential to separate regions destined for lysosomal degradation from carriers to be recycled to the plasma membrane (PubMed:30220460).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (3), as well as variants 10 and 11, encodes isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.