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Cbl c (CBLC) (NM_001130852) Human Tagged ORF Clone

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SKU
RC225700
CBLC (Myc-DDK-tagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Cbl c
Synonyms CBL-3; CBL-SL; RNF57
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC225700 representing NM_001130852
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGGCGGTGGCCCCGTGGGGGCGACAGTGGGAAGAGGCCCGCGCCCTGGGCCGGGCAGTCAGGA
TGCTGCAGCGCCTAGAAGAGCAATGCGTCGACCCCCGGCTGTCCGTGAGTCCCCCTTCGCTGCGGGACCT
GCTGCCCCGCACAGCGCAGCTGCTTCGAGAGGTGGCCCATTCTCGGCGGGCGGCCGGCGGAGGCGGCCCC
GGGGGTCCCGGCGGCTCTGGGGACTTTCTACTCATCTACCTGGCCAATCTGGAGGCCAAGAGCAGGCAGG
TGGCCGCGCTGCTGCCTCCCCGGGGCCGAAGGAGTGCCAACGACGAGCTCTTCCGGGCGGGCTCCAGACT
CAGGCGACAGCTGGCCAAGCTGGCCATCATCTTCAGCCACATGCACGCAGAGCTGCACGCACTCTTCCCC
GGGGGAAAGTACTGTGGACACATGTACCAGCTCACCAAGGCCCCCGCCCACACCTTCTGGAGGGAAAGTT
GCGGAGCCCGGTGTGTGCTGCCCTGGGCTGAGTTTGAGTCCCTCCTGGGCACCTGCCACCCTGTGGAACC
AGGCTGCACAGCCCTGGCCTTGCGCACCACCATTGACCTCACCTGCAGCGGGCACGTGTCCATCTTCGAG
TTCGACGTCTTCACCAGGCTCTTTCAGCCATGGCCAACACTCCTCAAGAACTGGCAGCTCCTGGCAGTCA
ACCACCCAGGCTACATGGCCTTCCTCACCTATGATGAGGTCCAAGAGCGTCTGCAGGCCTGCAGGGACAA
GCCAGGCAGCTACCTCTACCCAGATGGAAAGACCCACAACCCAGACCTGACTGAGCTCGGCCAGGCAGAA
CCCCAGCAGCGCATCCACGTGTCAGAGGAGCAGCTGCAGCTCTACTGGGCCATGGACTCCACATTTGAGC
TCTGCAAGATCTGTGCTGAGAGCAACAAGGATGTGAAGATTGAGCCGTGCGGGCACCTGCTCTGCAGCTG
CTGCCTGGCTGCCTGGCAGCACTCGGACAGCCAGACCTGCCCCTTCTGCCGCTGCGAGATCAAGGGCTGG
GAGGCCGTGAGTATCTACCAGTTCCACGGTCAGGCTACTGCTGAGGACTCAGGGAACAGCAGTGACCAGG
AAGGCAGGGAGTTGGAGCTGGGGCAGGTGCCCCTTTCGGCTCCTCCATTGCCCCCACGGCCAGATCTGCC
CCCCAGGAAGCCCAGAAATGCCCAGCCGAAAGTGAGACTCCTAAAGGGGAACTCCCCTCCAGCTGCGCTG
GGACCCCAGGACCCTGCCCCGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC225700 representing NM_001130852
Red=Cloning site Green=Tags(s)

MALAVAPWGRQWEEARALGRAVRMLQRLEEQCVDPRLSVSPPSLRDLLPRTAQLLREVAHSRRAAGGGGP
GGPGGSGDFLLIYLANLEAKSRQVAALLPPRGRRSANDELFRAGSRLRRQLAKLAIIFSHMHAELHALFP
GGKYCGHMYQLTKAPAHTFWRESCGARCVLPWAEFESLLGTCHPVEPGCTALALRTTIDLTCSGHVSIFE
FDVFTRLFQPWPTLLKNWQLLAVNHPGYMAFLTYDEVQERLQACRDKPGSYLYPDGKTHNPDLTELGQAE
PQQRIHVSEEQLQLYWAMDSTFELCKICAESNKDVKIEPCGHLLCSCCLAAWQHSDSQTCPFCRCEIKGW
EAVSIYQFHGQATAEDSGNSSDQEGRELELGQVPLSAPPLPPRPDLPPRKPRNAQPKVRLLKGNSPPAAL
GPQDPAPA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001130852
ORF Size 1284 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001130852.1, NP_001124324.1
RefSeq ORF 1287 bp
Locus ID 23624
UniProt ID Q9ULV8, Q9ULV8-2
Cytogenetics 19q13.32
Protein Families Druggable Genome
Protein Pathways Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
MW 47.2 kDa
Summary This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Feb 2012
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Documents
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"NM_001130852" in other vectors (4)
SKU Description Size Price
RC225700L3 Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC225700L4 Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 2, mGFP tagged 10 ug
$757.00
RG225700 CBLC (tGFP-tagged) - Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 2 10 ug
$657.00
SC325915 CBLC (untagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 2 10 ug
$503.00

Plasmid Map for RC225700

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC225700
Circular map for RC225700

file-alt Citations

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