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SUMF2 (NM_001130069) Human Tagged ORF Clone

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SKU
RC225532
SUMF2 (Myc-DDK-tagged)-Human sulfatase modifying factor 2 (SUMF2), transcript variant 5
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SUMF2
Synonyms pFGE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC225532 representing NM_001130069
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGCGCATGCGCAGCGGGGCCGTGGGTGTACGCGGCGCAGCGCGGCAGTCCTGATGGCCCGGCATG
GGTTACCGCTGCTGCCCCTGCTGTCGCTCCTGGTCGGCGCGTGGCTCAAGCTAGGAAATGGACAGGCTAC
TAGCATGGTCCAACTGCAGGGTGGGAGATTCCTGATGGGAACAAATTCTCCAGACAGCAGAGATGGTGAA
GGGCCTGTGCGGGAGGCGACAGTGAAACCCTTTGCCATCGACATATTTCCTGTCACCAACAAAGATTTCA
GGGATTTTGTCAGGGAGAAAAAGTATCGGACAGAAGCTGAGATGTTTGGATGGAGCTTTGTCTTTGAGGA
CTTTGTCTCTGATGAGCTGAGAAACAAAGCCACCCAGCCAATGAAGTCTGTACTCTGGTGGCTTCCAGTG
GAAAAGGCATTTTGGAGGCAGCCTGCAGGTCCTGGCTCTGGCATCCGAGAGAGACTGGAGCACCCAGTGT
TACACGTGAGCTGGAATGACGCCCGTGCCTACTGTGCTTGGCGGGGAAAACGACTGCCCACGGAGGAAGA
GTGGGAGTTTGCCGCCCGAGGGGGCTTGAAGGGTCAAGTTTACCCATGGGGGAACTGGTTCCAGCCAAAC
CGCACCAACCTGTGGCAGGGAAAGTTCCCCAAGGGAGACAAAGCTGAGGATGGCTTCCATGGAGTCTCCC
CAGTGAATGCTTTCCCCGCCCAGAACAACTACGGATGGGCAACACTCCAGATTCAGCCTCAGACAACCTC
GGTTTCCGCTGTGCTGCAGACGCAGGCCGGCCGCCAGGGGAGCTGTAAGCAGCCGGGTGGTGACAAGGAG
AAAAGCCTTCTAGGGTCACTGTCATTCCCTGGCCATGTTGCAAACAGCGCAATTCCAAGCTCGAGAGCTT
CAGCCTCAGGAAAGAACTTCCCCTTCCCTGTCTCCCATCCCTCTGTGGCAGGCGCCTCTCACCAGGGCAG
GAGAGGACTCAGCCTCCTGTGTTTTGGAGAAGGGGCCCAATGTGTGTTGACGATGGCTGGGGGCCAGGTG
TTTCTGTTAGAGGCCAAGTATTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC225532 representing NM_001130069
Red=Cloning site Green=Tags(s)

MRAHAQRGRGCTRRSAAVLMARHGLPLLPLLSLLVGAWLKLGNGQATSMVQLQGGRFLMGTNSPDSRDGE
GPVREATVKPFAIDIFPVTNKDFRDFVREKKYRTEAEMFGWSFVFEDFVSDELRNKATQPMKSVLWWLPV
EKAFWRQPAGPGSGIRERLEHPVLHVSWNDARAYCAWRGKRLPTEEEWEFAARGGLKGQVYPWGNWFQPN
RTNLWQGKFPKGDKAEDGFHGVSPVNAFPAQNNYGWATLQIQPQTTSVSAVLQTQAGRQGSCKQPGGDKE
KSLLGSLSFPGHVANSAIPSSRASASGKNFPFPVSHPSVAGASHQGRRGLSLLCFGEGAQCVLTMAGGQV
FLLEAKYY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001130069
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001130069.2, NP_001123541.1
RefSeq ORF 1020 bp
Locus ID 25870
UniProt ID Q8NBJ7
Cytogenetics 7p11.2
MW 39.49 kDa
Summary The catalytic sites of sulfatases are only active if they contain a unique amino acid, C-alpha-formylglycine (FGly). The FGly residue is posttranslationally generated from a cysteine by enzymes with FGly-generating activity. The gene described in this record is a member of the sulfatase-modifying factor family and encodes a protein with a DUF323 domain that localizes to the lumen of the endoplasmic reticulum. This protein has low levels of FGly-generating activity but can heterodimerize with another family member - a protein with high levels of FGly-generating activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. provided by RefSeq, Jul 2008
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"NM_001130069" in other vectors (4)
SKU Description Size Price
RC225532L3 Lenti ORF clone of Human sulfatase modifying factor 2 (SUMF2), transcript variant 5, Myc-DDK-tagged 10 ug
$757.00
RC225532L4 Lenti ORF clone of Human sulfatase modifying factor 2 (SUMF2), transcript variant 5, mGFP tagged 10 ug
$757.00
RG225532 SUMF2 (tGFP-tagged) - Human sulfatase modifying factor 2 (SUMF2), transcript variant 5 10 ug
$657.00
SC325816 SUMF2 (untagged)-Human sulfatase modifying factor 2 (SUMF2), transcript variant 5 10 ug
$503.00

Plasmid Map for RC225532

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC225532
Circular map for RC225532

file-alt Citations

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