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CDC23 (NM_004661) Human Tagged ORF Clone

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SKU
RC224973
CDC23 (Myc-DDK-tagged)-Human cell division cycle 23 homolog (S. cerevisiae) (CDC23)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $556.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDC23
Synonyms ANAPC8; APC8; CUT23
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224973 representing NM_004661
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGAGTACCTCCATGGTCCCGGTGGCTGTGACGGCGGCAGTGGCGCCTGTCCTGTCCATAAACA
GCGATTTCTCAGATTTGCGGGAAATTAAAAAGCAACTGCTGCTTATTGCGGGCCTTACCCGGGAGCGGGG
CCTACTACACAGTAGCAAATGGTCGGCGGAGTTGGCTTTCTCTCTCCCTGCATTGCCTCTGGCCGAGCTG
CAACCGCCTCCGCCTATTACAGAGGAAGATGCCCAGGATATGGATGCCTATACCCTGGCCAAGGCCTACT
TTGACGTTAAAGAGTATGATCGGGCAGCACATTTCCTGCATGGCTGCAATAGCAAGAAAGCCTATTTTCT
GTATATGTATTCCAGATATCTGTCTGGAGAAAAAAAGAAGGACGATGAAACAGTTGATAGCTTAGGCCCC
CTGGAAAAAGGACAAGTGAAAAATGAGGCGCTTAGAGAATTGAGAGTGGAGCTCAGCAAAAAACACCAAG
CTCGAGAACTTGATGGATTTGGACTTTATCTGTATGGTGTGGTGCTTCGAAAACTGGACTTGGTTAAAGA
GGCCATTGATGTGTTTGTGGAAGCTACTCATGTTTTGCCCTTGCATTGGGGAGCCTGGTTAGAACTCTGT
AACCTGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCCAGACACCTGGATGAAAGAGTTTTTTC
TGGCTCATATATACACAGAGTTGCAGTTGATAGAGGAGGCCCTGCAAAAGTATCAGAATCTCATTGATGT
GGGCTTCTCTAAGAGCTCGTATATTGTTTCCCAAATTGCAGTTGCCTATCACAATATCAGAGATATTGAC
AAAGCCCTCTCCATTTTTAATGAGCTAAGGAAACAAGACCCTTACAGGATTGAAAATATGGACACATTCT
CCAACCTTCTTTATGTCAGGAGCATGAAATCGGAGTTGAGTTATCTGGCTCATAACCTCTGTGAGATTGA
TAAATATCGTGTAGAAACGTGCTGTGTAATTGGCAATTATTACAGTTTACGTTCTCAGCATGAGAAAGCA
GCCTTATATTTCCAGAGAGCCCTGAAATTAAATCCTCGGTATCTTGGTGCCTGGACACTAATGGGACATG
AGTACATGGAGATGAAGAACACGTCTGCTGCTATCCAGGCTTATAGACATGCCATTGAGGTCAACAAACG
GGACTACAGAGCTTGGTATGGCCTCGGGCAGACCTATGAAATCCTTAAGATGCCATTTTACTGCCTTTAT
TATTATAGACGGGCCCACCAGCTTCGACCCAATGATTCTCGCATGCTGGTTGCTTTAGGAGAATGTTACG
AGAAACTCAATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCTTACGCCGTGGGAGATGTGGAGAA
AATGGCTCTGGTGAAACTGGCAAAGCTTCATGAACAGTTGACTGAGTCAGAACAGGCTGCCCAGTGTTAC
ATCAAATATATCCAAGATATCTATTCCTGTGGGGAAATAGTAGAACACTTGGAGGAAAGCACTGCCTTTC
GCTATCTGGCCCAGTACTATTTTAAGTGCAAACTGTGGGATGAAGCTTCAACTTGTGCACAAAAGTGTTG
TGCATTTAATGATACCCGGGAAGAAGGTAAGGCCTTACTCCGGCAAATCCTACAGCTTCGGAACCAAGGC
GAGACTCCTACCACCGAGGTGCCTGCTCCCTTTTTCCTACCTGCTTCACTCTCTGCTAACAATACCCCCA
CACGCAGAGTTTCTCCACTCAACTTGTCTTCTGTCACGCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224973 representing NM_004661
Red=Cloning site Green=Tags(s)

MAASTSMVPVAVTAAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLAEL
QPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGP
LEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFVEATHVLPLHWGAWLELC
NLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQNLIDVGFSKSSYIVSQIAVAYHNIRDID
KALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKA
ALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLY
YYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCY
IKYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQG
ETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004661
ORF Size 1791 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004661.4
RefSeq Size 3169 bp
RefSeq ORF 1794 bp
Locus ID 8697
UniProt ID Q9UJX2
Cytogenetics 5q31.2
Domains APC8, TPR
Protein Families Druggable Genome
Protein Pathways Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
MW 68.7 kDa
Summary The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. provided by RefSeq, Jul 2008
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"NM_004661" in other vectors (7)
SKU Description Size Price
RC224973L1 Lenti ORF clone of Human cell division cycle 23 homolog (S. cerevisiae) (CDC23), Myc-DDK-tagged 10 ug
$856.00
RC224973L2 Lenti ORF clone of Human cell division cycle 23 homolog (S. cerevisiae) (CDC23), mGFP tagged 10 ug
$856.00
RC224973L3 Lenti ORF clone of Human cell division cycle 23 homolog (S. cerevisiae) (CDC23), Myc-DDK-tagged 10 ug
$856.00
RC224973L4 Lenti ORF clone of Human cell division cycle 23 homolog (S. cerevisiae) (CDC23), mGFP tagged 10 ug
$856.00
RG224973 CDC23 (tGFP-tagged) - Human cell division cycle 23 homolog (S. cerevisiae) (CDC23) 10 ug
$489.00 $756.00
SC117209 CDC23 (untagged)-Human cell division cycle 23 homolog (S. cerevisiae) (CDC23) 10 ug
$551.00
SC318029 CDC23 (untagged)-Human cell division cycle 23 homolog (S. cerevisiae) (CDC23) 10 ug
$612.00

Plasmid Map for RC224973

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224973
Circular map for RC224973

file-alt Citations

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