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TLCD3A (NM_024792) Human Tagged ORF Clone

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SKU
RC224943
FAM57A (Myc-DDK-tagged)-Human family with sequence similarity 57, member A (FAM57A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "TLCD3A or NM_024792"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TLCD3A
Synonyms CT120; FAM57A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224943 representing NM_024792
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTGACGCTGGCCGGGGGCGCGCTCTTCTTCCCGGGGCTCTTCGCGCTCTGCACCTGGGCGCTGC
GCCGCTCCCAGCCCGGATGGAGCCGCACCGACTGCGTGATGATCAGCACCAGGCTGGTTTCCTCGGTGCA
CGCCGTGCTGGCCACCGGCTCGGGGATCGTCATCATTCGCTCCTGCGACGACGTGATCACCGGCAGGCAC
TGGCTTGCCCGGGAATATGTGTGGTTTCTGATTCCATACATGATCTATGACTCGTACGCCATGTACCTCT
GTGAATGGTGCCGAACCAGAGACCAGAACCGTGCGCCCTCCCTCACTCTTCGAAACTTCCTAAGTCGAAA
CCGCCTCATGATCACACATCATGCGGTCATTCTCTTTGTCCTTGTGCCAGTCGCACAGAGGCTCCGGGGA
GACCTTGGGGACTTCTTTGTCGGCTGCATCTTCACGGCAGAACTGAGCACTCCGTTTGTGTCGCTGGGCA
GGGTTCTGATTCAGCTAAAGCAGCAGCACACCCTTCTGTACAAGGTGAATGGAATCCTCACGCTGGCCAC
CTTCCTTTCCTGCCGGATCCTTCTCTTCCCCTTCATGTACTGGTCCTATGGCCGCCAGCAGGGACTAAGC
CTGCTCCAAGTACCCTTCAGCATCCCATTCTACTGCAACGTGGCCAATGCCTTCCTCGTAGCTCCTCAGA
TCTACTGGTTCTGTCTGCTGTGCAGGAAGGCAGTCCGGCTCTTTGACACTCCCCAAGCCAAAAAGGATGG
C

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224943 representing NM_024792
Red=Cloning site Green=Tags(s)

MLLTLAGGALFFPGLFALCTWALRRSQPGWSRTDCVMISTRLVSSVHAVLATGSGIVIIRSCDDVITGRH
WLAREYVWFLIPYMIYDSYAMYLCEWCRTRDQNRAPSLTLRNFLSRNRLMITHHAVILFVLVPVAQRLRG
DLGDFFVGCIFTAELSTPFVSLGRVLIQLKQQHTLLYKVNGILTLATFLSCRILLFPFMYWSYGRQQGLS
LLQVPFSIPFYCNVANAFLVAPQIYWFCLLCRKAVRLFDTPQAKKDG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024792
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024792.3
RefSeq Size 2096 bp
RefSeq ORF 774 bp
Locus ID 79850
UniProt ID Q8TBR7
Cytogenetics 17p13.3
Domains TLC
Protein Families Druggable Genome, Transmembrane
MW 29.2 kDa
Summary The protein encoded by this gene is a membrane-associated protein that promotes lung carcinogenesis. The encoded protein may be involved in amino acid transport and glutathione metabolism since it can interact with a solute carrier family member (SLC3A2) and an isoform of gamma-glutamyltranspeptidase-like 3. An alternatively spliced variant encoding a protein that lacks a 32 aa internal segment showed the opposite effect, inhibiting lung cancer cell growth. Knockdown of this gene also inhibited lung carcinogenesis and tumor cell growth. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Dec 2015
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"NM_024792" in other vectors (4)
SKU Description Size Price
RC224943L3 Lenti ORF clone of Human family with sequence similarity 57, member A (FAM57A), Myc-DDK-tagged 10 ug
$600.00
RC224943L4 Lenti ORF clone of Human family with sequence similarity 57, member A (FAM57A), mGFP tagged 10 ug
$600.00
RG224943 FAM57A (tGFP-tagged) - Human family with sequence similarity 57, member A (FAM57A) 10 ug
$489.00 $500.00
SC112017 FAM57A (untagged)-Human family with sequence similarity 57, member A (FAM57A) 10 ug
$300.00

Plasmid Map for RC224943

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224943
Circular map for RC224943

file-alt Citations

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