OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Apc6 (CDC16) (NM_001078645) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC224869
CDC16 (Myc-DDK-tagged)-Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $577.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA350739 Rabbit Polyclonal Anti-CDC16 Antibody
100 ul
$205.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Apc6 or NM_001078645"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Apc6
Synonyms ANAPC6; APC6; CDC16Hs; CUT9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224869 representing NM_001078645
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCTAGAGCGGCTGCGGAAGCGCGTCCGGCAGTACCTCGACCAGCAACAGTATCAAAGTGCTCTAT
TTTGGGCAGATAAAGTAGCTTCACTCTCTCGTGAAGAACCCCAGGACATCTATTGGTTGGCTCAATGTCT
TTACCTGACAGCACAATATCACAGAGCCGCCCATGCACTTCGGTCACGAAAACTGGACAAATTGTATGAA
GCATGTCGTTACCTTGCAGCTAGGTGCCATTATGCTGCAAAAGAGCACCAGCAGGCCCTTGATGTTCTTG
ACATGGAAGAGCCCATCAATAAAAGATTATTTGAAAAATACTTGAAGGATGAAAGTGGCTTCAAAGATCC
CTCCAGCGACTGGGAAATGTCACAGTCTTCAATAAAGAGTTCTATCTGTCTTCTACGCGGGAAAATCTAT
GATGCTCTAGATAACCGAACCCTGGCTACCTACAGCTACAAAGAAGCTTTGAAGCTTGATGTCTACTGTT
TTGAAGCGTTCGATCTTTTAACATCACATCACATGCTGACAGCACAAGAAGAAAAAGAACTTCTTGAATC
ACTACCCCTTAGCAAGCTGTGTAATGAAGAACAGGAATTGCTGCGTTTTCTATTTGAGAACAAATTGAAA
AAATATAATAAGCCTAGTGAAACGGTCATCCCTGAATCTGTAGATGGCTTGCAAGAGAATCTGGATGTGG
TAGTGTCTTTAGCTGAGAGACATTATTATAACTGTGATTTTAAAATGTGCTACAAGCTTACTTCTGTAGT
AATGGAGAAAGATCCTTTCCATGCAAGTTGTTTACCTGTACATATAGGGACGCTTGTAGAGCTGAATAAA
GCCAATGAACTTTTCTATCTTTCTCATAAACTGGTGGATTTATATCCTAGTAATCCTGTGTCTTGGTTTG
CAGTGGGATGTTACTATCTCATGGTCGGTCATAAAAATGAACATGCCAGAAGATATCTCAGCAAAGCCAC
AACACTTGAGAAAACCTATGGACCTGCATGGATAGCCTATGGACATTCATTTGCGGTGGAGAGTGAGCAC
GACCAAGCGATGGCTGCTTACTTCACAGCAGCACAGCTGATGAAAGGGTGTCATTTGCCTATGCTGTATA
TTGGATTAGAATATGGTTTGACCAATAACTCAAAACTAGCTGAAAGGTTCTTCAGCCAAGCTCTGAGCAT
TGCACCGGAAGACCCTTTTGTTATGCATGAGGTCGGCGTGGTTGCATTTCAGAATGGAGAATGGAAAACA
GCCGAAAAATGGTTTCTTGATGCTTTGGAAAAAATTAAAGCAATTGGGAACGAGGTAACAGTTGACAAAT
GGGAACCTTTGTTGAACAACTTGGGGCATGTCTGCAGAAAACTTAAAAAGTATGCTGAGGCCTTGGATTA
CCACCGTCAGGCACTGGTGTTGATTCCTCAGAACGCATCCACCTACTCTGCTATTGGATATATCCACAGT
CTGATGGGCAACTTTGAAAATGCTGTGGACTACTTCCACACAGCCCTTGGTCTTAGGCGAGATGATACAT
TTTCTGTTACAATGCTTGGTCATTGCATCGAAATGTACATTGGTGATTCTGAAGCTTATATCGGAGCAGA
CATTAAAGACAAATTAAAATGTTATGACTTTGATGTGCATACAATGAAGACACTAAAAAACATTATTTCA
CCTCCGTGGGATTTCAGGGAATTTGAAGTAGAAAAACAGACTGCAGAAGAAACGGGGCTTACGCCATTGG
AAACCTCAAGGAAAACTCCAGATTCCAGACCTTCCTTGGAAGAAACCTTTGAAATTGAAATGAATGAAAG
TGACATGATGTTAGAGACATCTATGTCAGACCACAGCACG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224869 representing NM_001078645
Red=Cloning site Green=Tags(s)

MNLERLRKRVRQYLDQQQYQSALFWADKVASLSREEPQDIYWLAQCLYLTAQYHRAAHALRSRKLDKLYE
ACRYLAARCHYAAKEHQQALDVLDMEEPINKRLFEKYLKDESGFKDPSSDWEMSQSSIKSSICLLRGKIY
DALDNRTLATYSYKEALKLDVYCFEAFDLLTSHHMLTAQEEKELLESLPLSKLCNEEQELLRFLFENKLK
KYNKPSETVIPESVDGLQENLDVVVSLAERHYYNCDFKMCYKLTSVVMEKDPFHASCLPVHIGTLVELNK
ANELFYLSHKLVDLYPSNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEH
DQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGEWKT
AEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNASTYSAIGYIHS
LMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKDKLKCYDFDVHTMKTLKNIIS
PPWDFREFEVEKQTAEETGLTPLETSRKTPDSRPSLEETFEIEMNESDMMLETSMSDHST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001078645
ORF Size 1860 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001078645.3
RefSeq Size 2317 bp
RefSeq ORF 1863 bp
Locus ID 8881
UniProt ID Q13042, A0A024RDZ2
Cytogenetics 13q34
Protein Families Druggable Genome
Protein Pathways Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
MW 71.5 kDa
Summary The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. provided by RefSeq, Jan 2016
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001078645" in other vectors (4)
SKU Description Size Price
RC224869L3 Lenti ORF clone of Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2, Myc-DDK-tagged 10 ug
$877.00
RC224869L4 Lenti ORF clone of Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2, mGFP tagged 10 ug
$877.00
RG224869 CDC16 (tGFP-tagged) - Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2 10 ug
$489.00 $777.00
SC315597 CDC16 (untagged)-Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2 10 ug
$636.00

Plasmid Map for RC224869

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224869
Circular map for RC224869

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products