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SPAG11B (NM_058201) Human Tagged ORF Clone

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SKU
RC224764
SPAG11B (Myc-DDK-tagged)-Human sperm associated antigen 11B (SPAG11B), transcript variant D
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "SPAG11B or NM_058201"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPAG11B
Synonyms EDDM2B; EP2; EP2C; EP2D; HE2; HE2C; SPAG11; SPAG11A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224764 representing NM_058201
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCAACGATTGCTCCCGTCCGTCACCAGCCTTCTCCTTGTGGCCCTGCTGTTTCCAGGATCGTCTC
AAGCCAGACATGTGAACCACTCAGCCACTGAGGCTCTCGGAGAACTCAGGGAAAGAGCCCCTGGGCAAGG
CACAAACGGGTTTCAGCTGCTACGCCACGCAGTGAAACGGGACCTCTTACCACCGCGCACCCCACCTTAC
CAAGGGGATGTTCCACCGGGAATTAGAAATACCATCTGCCGTATGCAGCAAGGGATCTGCAGACTTTTTT
TCTGCCATTCTGGTGAGAAAAAGCGTGACATTTGCTCTGATCCCTGGAATAGGTGTTGCGTATCAAATAC
AGATGAAGAAGGAAAAGAGAAACCAGAGATGGATGGCAGATCTGGGATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224764 representing NM_058201
Red=Cloning site Green=Tags(s)

MRQRLLPSVTSLLLVALLFPGSSQARHVNHSATEALGELRERAPGQGTNGFQLLRHAVKRDLLPPRTPPY
QGDVPPGIRNTICRMQQGICRLFFCHSGEKKRDICSDPWNRCCVSNTDEEGKEKPEMDGRSGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_058201
ORF Size 399 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_058201.1, NP_478108.1
RefSeq Size 718 bp
RefSeq ORF 402 bp
Locus ID 10407
UniProt ID Q08648
Cytogenetics 8p23.1
Protein Families Secreted Protein
MW 12.1 kDa
Summary This gene encodes several androgen-dependent, epididymis-specific secretory proteins. The specific functions of these proteins have not been determined, but they are thought to be involved in sperm maturation. Some of the isoforms contain regions of similarity to beta-defensins, a family of antimicrobial peptides. The gene is located on chromosome 8p23 near the defensin gene cluster. Alternative splicing of this gene results in seven transcript variants encoding different isoforms. Two different N-terminal and five different C-terminal protein sequences are encoded by the splice variants. Two additional variants have been described, but their full length sequences have not been determined. provided by RefSeq, Jul 2008
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"NM_058201" in other vectors (4)
SKU Description Size Price
RC224764L3 Lenti ORF clone of Human sperm associated antigen 11B (SPAG11B), transcript variant D, Myc-DDK-tagged 10 ug
$450.00
RC224764L4 Lenti ORF clone of Human sperm associated antigen 11B (SPAG11B), transcript variant D, mGFP tagged 10 ug
$450.00
RG224764 SPAG11B (tGFP-tagged) - Human sperm associated antigen 11B (SPAG11B), transcript variant D 10 ug
$350.00
SC305769 SPAG11B (untagged)-Human sperm associated antigen 11B (SPAG11B), transcript variant D 10 ug
$165.00

Plasmid Map for RC224764

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224764
Circular map for RC224764

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