DBX1 (NM_001029865) Human Tagged ORF Clone

  • TrueORF®
SKU
RC224662
DBX1 (Myc-DDK-tagged)-Human developing brain homeobox 1 (DBX1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DBX1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224662 representing NM_001029865
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTTCCCCGGCCTCCTCGCGCCCCCCGCCGGGTACCCTAGCCTCCTGCGGCCCACGCCCACCTTGA
CGCTGCCCCAGTCCTTGCAGTCGGCATTTTCCGGCCACTCCAGCTTCCTGGTGGAGGATCTGATCCGCAT
CAGCCGACCCCCCGCCTACCTGCCCCGCAGCGTGCCCACCGCCAGCATGTCGCCGCCCAGGCAGGGGGCC
CCCACGGCCCTCACCGACACGGGGGCCTCGGACCTGGGCTCCCCGGGTCCCGGCAGCCGACGGGGCGGCT
CTCCGCCGACTGCCTTCTCCCCTGCCAGCGAGACGACGTTTCTGAAGTTTGGAGTGAACGCCATCCTCTC
CTCGGGGCCCAGAACAGAAACATCCCCAGCCTTGCTCCAGAGCGTCCCTCCCAAGACCTTCGCCTTTCCC
TACTTCGAAGGGTCTTTTCAGCCTTTCATCAGATCTTCTTATTTCCCAGCTTCCTCCAGCGTGGTGCCCA
TCCCCGGGACCTTCTCCTGGCCGCTGGCCGCGCGCGGGAAGCCTCGGCGGGGCATGCTGCGTCGAGCAGT
CTTCTCCGACGTGCAGCGGAAGGCGCTGGAGAAGATGTTCCAGAAGCAGAAGTACATCAGCAAGCCCGAC
CGCAAGAAGCTGGCGGCCAAGCTGGGCCTGAAAGACTCGCAGGACACAGCCCGAAGGACAGAGCCTGCAC
CCGACTCCACTCCCCGCCCCCGTGCGTCCCCCGGAGCCCCTGATCTGACCTCTGCTTCTCCCTTCTTCTC
GCCGGCCGGGACATTCCAGGTGAAAATCTGGTTCCAGAACCGACGCATGAAATGGCGGAACTCCAAGGAG
CGCGAACTCCTGTCTAGCGGGGGCTGTCGCGAGCAGACCCTGCCCACCAAGCTCAATCCGCACCCGGACC
TCAGCGACGTGGGCCAGAAGGGCCCTGGGAACGAAGAGGAGGAGGAGGGCCCGGGCAGCCCCAGCCACCG
CCTGGCCTACCACGCGTCCTCCGACCCCCAGCACCTGCGGGACCCGCGGCTGCCAGGGCCGCTGCCCCCC
TCGCCCGCGCACTCGAGCAGTCCCGGGAAACCTTCGGACTTCTCAGATTCCGAGGAGGAAGAGGAGGGCG
AGGAACAGGAGGAAATCACCGTGTCC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC224662 representing NM_001029865
Red=Cloning site Green=Tags(s)

MMFPGLLAPPAGYPSLLRPTPTLTLPQSLQSAFSGHSSFLVEDLIRISRPPAYLPRSVPTASMSPPRQGA
PTALTDTGASDLGSPGPGSRRGGSPPTAFSPASETTFLKFGVNAILSSGPRTETSPALLQSVPPKTFAFP
YFEGSFQPFIRSSYFPASSSVVPIPGTFSWPLAARGKPRRGMLRRAVFSDVQRKALEKMFQKQKYISKPD
RKKLAAKLGLKDSQDTARRTEPAPDSTPRPRASPGAPDLTSASPFFSPAGTFQVKIWFQNRRMKWRNSKE
RELLSSGGCREQTLPTKLNPHPDLSDVGQKGPGNEEEEEGPGSPSHRLAYHASSDPQHLRDPRLPGPLPP
SPAHSSSPGKPSDFSDSEEEEEGEEQEEITVS

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001029865
ORF Size 1146 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001029865.1, NP_001025036.1
RefSeq Size 1032 bp
RefSeq ORF 1032 bp
Locus ID 120237
UniProt ID A6NMT0
Cytogenetics 11p15.1
MW 41.3 kDa
Summary Could have a role in patterning the central nervous system during embryogenesis. Has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneurons, V0 and V1 neurons. Regulates the transcription factor profile, neurotransmitter phenotype, intraspinal migratory path and axonal trajectory of V0 neurons, features that differentiate them from an adjacent set of V1 neurons (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001029865" in other vectors (4)
SKU Description Size Price
RC224662L3 Lenti-ORF clone of DBX1 (Myc-DDK-tagged)-Human developing brain homeobox 1 (DBX1) 10 ug
$757.00
RC224662L4 Lenti-ORF clone of DBX1 (mGFP-tagged)-Human developing brain homeobox 1 (DBX1) 10 ug
$757.00
RG224662 DBX1 (tGFP-tagged) - Human developing brain homeobox 1 (DBX1) 10 ug
$657.00
SC302449 DBX1 (untagged)-Human developing brain homeobox 1 (DBX1) 10 ug
$457.00

Plasmid Map for RC224662

Download sequence for

Cloning scheme for RC224662

Circular map for RC224662

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