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PCDHGA11 (NM_032092) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC224531
PCDHGA11 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $755.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PCDHGA11
Synonyms PCDH-GAMMA-A11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224531 representing NM_032092
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAATCGGCTACAGCGCGGGGACCGCAGTCGGCTGCTGCTGCTGCTGTGCATTTTCCTGGGGACGC
TGCGGGGGTTCCGGGCCAGGCAGATCCGATATTCGGTGCCAGAAGAGACCGAAAAGGGCTCCTTCGTGGG
CAATATCTCCAAGGACCTGGGGCTGGAGCCCCGGGAGCTGGCGAAGCGCGGAGTCCGCATCGTCTCCAGA
GGGAAGACACAGCTTTTCGCTGTGAATCCGCGAAGCGGCAGCTTGATCACGGCAGGCAGGATAGACCGGG
AGGAGCTCTGTGAGACGGTGTCCTCCTGTTTTTTAAATATGGAACTTCTCGTGGAAGACACCTTGAAGAT
TTACGGAGTGGAGGTGGAAATAATAGATATTAATGATAACGCCCCCAGCTTCCAGGAGGACGAAGTGGAG
ATAAAAGTCAGTGAGCACGCAATTCCTGGGGCGCGATTTGCTCTTCCTAATGCTAGGGATCCAGATGTGG
GCGTGAACTCCCTCCAGAGCTACCAGCTCAGCCCTAATAATTACTTTTCCTTGCAACTGCGGGGCAGAAC
GGATGGGGCCAAGAATCCAGAGCTAGTACTGGAGGGAAGCCTGGACCGAGAGAAAGAGGCTGCTCACCTG
CTCCTCCTCACAGCTTTAGATGGAGGCGATCCCATCCGAAAGGGCGCAGTTCCCATTCGTGTGGTGGTCC
TCGATGTAAATGATCACATCCCAATGTTTACACAGTCCGTATATCGCGTGAGTGTTCCAGAAAACATCAG
CTCCGGAACTCGGGTGCTGATGGTTAATGCAACGGATCCAGACGAGGGAATCAACGGGGAAGTAATGTAT
TCATTTCGGAACATGGAAAGCAAGGCTTCTGAAATATTCCAATTGGATTCACAAACTGGAGAAGTTCAAG
TACGGGGGTCTCTGGATTTTGAAAAATATAGATTCTATGAGATGGAAATTCAAGGCCAAGATGGTGGAGG
TCTCTTTACCACCACGACGATGTTGATCACTGTTGTGGATGTGAATGATAACGCTCCAGAAATAACTATC
ACCTCTTCTATTAATTCAATTCTGGAAAACTCTCCTCCAGGTACAGTGATTGCTCTTCTAAATGTGCAAG
ATCAAGATTCTGGAGAAAATGGTCAAGTCTCCTGTTTTATTCCTAACCACCTGCCTTTTAAATTAGAAAA
GACTTATGGAAATTATTACAAATTGATAACAAGCAGAGTGCTGGACAGGGAGTTGGTCCAGAGCTACAAT
ATAACGTTGACAGCCACAGACCAGGGAAGCCCGCCTTTGTCTGCAGAAACTCATGTCTGGCTGAATGTGG
CAGATGACAACGATAACCCTCCCGTTTTTCCTCACTCCTCTTACTCTGCCTACATTCCCGAAAACAACCC
CAGGGGTGCCTCCATCTTCTCAGTGACCGCCCTCGACCCGGACAGCAAACAGAATGCCCTGGTCACTTAC
TCTCTGACGGATGACACTGTCCAGGGGGTGCCTCTGTCCTCCTATGTCTCTATTAACTCCAACACTGGTG
TTCTCTATGCCCTACAATCCTTCGACTATGAGCAGTTTCGAGACTTAGAACTGAGAGTGATAGCACGTGA
CAGCGGGGACCCGCCCCTCAGCAGCAACGTGTCGCTGAGCCTGTTCGTGCTGGACCAGAACGACAATGCG
CCCGAGATCCTGTACCCTGCCCTCCCCACAGACGGCTCCACTGGCGTGGAGCTGGCGCCCCGCTCTGCGG
AACCTGGCTACCTGGTGACCAAGGTGGTTGCGGTGGACAAAGATTCAGGCCAGAACGCCTGGCTGTCCTA
TCGCCTGCTTAAGGCCAGCGAGCCGGGACTCTTCGCGGTGGGGGAGCACACGGGCGAGCAAGCCCCGCCC
AACACGGACTGGCGTTTCTCTCAGGCCCAGAGACCCGGCACCAGCGGCTCCCAAAATGGCGATGACACCG
GCACCTGGCCCAACAACCAGTTTGACACAGAGATGCTGCAAGCCATGATCTTGGCGTCCGCCAGTGAAGC
TGCTGATGGGAGCTCCACCCTGGGAGGGGGTGCCGGCACCATGGGATTGAGCGCCCGCTACGGACCCCAG
TTCACCCTGCAGCACGTGCCCGACTACCGCCAGAATGTCTACATCCCAGGCAGCAATGCCACACTGACCA
ACGCAGCTGGCAAGCGGGATGGCAAGGCCCCAGCAGGTGGCAATGGCAACAAGAAGAAGTCGGGCAAGAA
GGAGAAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224531 representing NM_032092
Red=Cloning site Green=Tags(s)

MANRLQRGDRSRLLLLLCIFLGTLRGFRARQIRYSVPEETEKGSFVGNISKDLGLEPRELAKRGVRIVSR
GKTQLFAVNPRSGSLITAGRIDREELCETVSSCFLNMELLVEDTLKIYGVEVEIIDINDNAPSFQEDEVE
IKVSEHAIPGARFALPNARDPDVGVNSLQSYQLSPNNYFSLQLRGRTDGAKNPELVLEGSLDREKEAAHL
LLLTALDGGDPIRKGAVPIRVVVLDVNDHIPMFTQSVYRVSVPENISSGTRVLMVNATDPDEGINGEVMY
SFRNMESKASEIFQLDSQTGEVQVRGSLDFEKYRFYEMEIQGQDGGGLFTTTTMLITVVDVNDNAPEITI
TSSINSILENSPPGTVIALLNVQDQDSGENGQVSCFIPNHLPFKLEKTYGNYYKLITSRVLDRELVQSYN
ITLTATDQGSPPLSAETHVWLNVADDNDNPPVFPHSSYSAYIPENNPRGASIFSVTALDPDSKQNALVTY
SLTDDTVQGVPLSSYVSINSNTGVLYALQSFDYEQFRDLELRVIARDSGDPPLSSNVSLSLFVLDQNDNA
PEILYPALPTDGSTGVELAPRSAEPGYLVTKVVAVDKDSGQNAWLSYRLLKASEPGLFAVGEHTGEQAPP
NTDWRFSQAQRPGTSGSQNGDDTGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQ
FTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032092
ORF Size 2250 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032092.2
RefSeq Size 4317 bp
RefSeq ORF 2253 bp
Locus ID 56105
UniProt ID Q9Y5H2
Cytogenetics 5q31.3
Protein Families Transmembrane
MW 78.7 kDa
Summary This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. provided by RefSeq, Jul 2008
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Documents
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"NM_032092" in other vectors (4)
SKU Description Size Price
RC224531L3 Lenti-ORF clone of PCDHGA11 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3 10 ug
$1,055.00
RC224531L4 Lenti-ORF clone of PCDHGA11 (mGFP-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3 10 ug
$1,055.00
RG224531 PCDHGA11 (tGFP-tagged) - Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3 10 ug
$955.00
SC110552 PCDHGA11 (untagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3 10 ug
$688.00

Plasmid Map for RC224531

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224531
Circular map for RC224531

file-alt Citations

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