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PCDHGA11 (NM_032092) Human Untagged Clone

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SKU
SC110552
PCDHGA11 (untagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3
  $688.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PCDHGA11
Synonyms PCDH-GAMMA-A11
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC110552 sequence for NM_032092 edited (data generated by NextGen Sequencing)
ATGGCGAATCGGCTACAGCGCGGGGACCGCAGTCGGCTGCTGCTGCTGCTGTGCATTTTC
CTGGGGACGCTGCGGGGGTTCCGGGCCAGGCAGATCCGATATTCGGTGCCAGAAGAGACC
GAAAAGGGCTCCTTCGTGGGCAATATCTCCAAGGACCTGGGGCTGGAGCCCCGGGAGCTG
GCGAAGCGCGGAGTCCGCATCGTCTCCAGAGGGAAGACACAGCTTTTCGCTGTGAATCCG
CGAAGCGGCAGCTTGATCACGGCAGGCAGGATAGACCGGGAGGAGCTCTGTGAGACGGTG
TCCTCCTGTTTTTTAAATATGGAACTTCTCGTGGAAGACACCTTGAAGATTTACGGAGTG
GAGGTGGAAATAATAGATATTAATGATAACGCCCCCAGCTTCCAGGAGGACGAAGTGGAG
ATAAAAGTCAGTGAGCACGCAATTCCTGGGGCGCGATTTGCTCTTCCTAATGCTAGGGAT
CCAGATGTGGGCGTGAACTCCCTCCAGAGCTACCAGCTCAGCCCTAATAATTACTTTTCC
TTGCAACTGCGGGGCAGAACGGATGGGGCCAAGAATCCAGAGCTAGTACTGGAGGGAAGC
CTGGACCGAGAGAAAGAGGCTGCTCACCTGCTCCTCCTCACAGCTTTAGATGGAGGCGAT
CCCATCCGAAAGGGCGCAGTTCCCATTCGTGTGGTGGTCCTCGATGTAAATGATCACATC
CCAATGTTTACACAGTCCGTATATCGCGTGAGTGTTCCAGAAAACATCAGCTCCGGAACT
CGGGTGCTGATGGTTAATGCAACGGATCCAGACGAGGGAATCAACGGGGAAGTAATGTAT
TCATTTCGGAACATGGAAAGCAAGGCTTCTGAAATATTCCAATTGGATTCACAAACTGGA
GAAGTTCAAGTACGGGGGTCTCTGGATTTTGAAAAATATAGATTCTATGAGATGGAAATT
CAAGGCCAAGATGGTGGAGGTCTCTTTACCACCACGACGATGTTGATCACTGTTGTGGAT
GTGAATGATAACGCTCCAGAAATAACTATCACCTCTTCTATTAATTCAATTCTGGAAAAC
TCTCCTCCAGGTACAGTGATTGCTCTTCTAAATGTGCAAGATCAAGATTCTGGAGAAAAT
GGTCAAGTCTCCTGTTTTATTCCTAACCACCTGCCTTTTAAATTAGAAAAGACTTATGGA
AATTATTACAAATTGATAACAAGCAGAGTGCTGGACAGGGAGTTGGTCCAGAGCTACAAT
ATAACGTTGACAGCCACAGACCAGGGAAGCCCGCCTTTGTCTGCAGAAACTCATGTCTGG
CTGAATGTGGCAGATGACAACGATAACCCTCCCGTTTTTCCTCACTCCTCTTACTCTGCC
TACATTCCCGAAAACAACCCCAGGGGTGCCTCCATCTTCTCAGTGACCGCCCTCGACCCG
GACAGCAAACAGAATGCCCTGGTCACTTACTCTCTGACGGATGACACTGTCCAGGGGGTG
CCTCTGTCCTCCTATGTCTCTATTAACTCCAACACTGGTGTTCTCTATGCCCTACAATCC
TTCGACTATGAGCAGTTTCGAGACTTAGAACTGAGAGTGATAGCACGTGACAGCGGGGAC
CCGCCCCTCAGCAGCAACGTGTCGCTGAGCCTGTTCGTGCTGGACCAGAACGACAATGCG
CCCGAGATCCTGTACCCTGCCCTCCCCACAGACGGCTCCACTGGCGTGGAGCTGGCGCCC
CGCTCTGCGGAACCTGGCTACCTGGTGACCAAGGTGGTTGCGGTGGACAAAGATTCAGGC
CAGAACGCCTGGCTGTCCTATCGCCTGCTTAAGGCCAGCGAGCCGGGACTCTTCGCGGTG
GGGGAGCACACGGGCGAGNNNGCCCCGCCCAACACGGACTGGCGTTTCTCTCAGGCCCAG
AGACCCGGCACCAGCGGCTCCCAAAATGGCGATGACACCGGCACCTGGCCCAACAACCAG
TTTGACACAGAGATGCTGCAAGCCATGATCTTGGCGTCCGCCAGTGAAGCTGCTGATGGG
AGCTCCACCCTGGGAGGGGGTGCCGGCACCATGGGATTGAGCGCCCGCTACGGACCCCAG
TTCACCCTGCAGCACGTGCCCGACTACCGCCAGAATGTCTACATCCCAGGCAGCAATGCC
ACACTGACCAACGCAGCTGGCAAGCGGGATGGCAAGGCCCCAGCAGGTGGCAATGGCAAC
AAGAAGAAGTCGGGCAAGAAGGAGAAGAAGTAA

Clone variation with respect to NM_032092.1
1879 c=>n;1880 a=>n;1881 a=>n
5' Read Nucleotide Sequence
>OriGene 5' read for NM_032092 unedited
NNNAATGTTCGNAATTTGTNATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGT
GGATACTCACANATCCTGACACTGGAGACTTAGAAGTATTTTCCTTCGCTTTCTGATATA
TTTTGGATGTAGTCGGCCTAGGACTTCATAGATACATAAGCCGATTCACAACCAACCAGC
TCGAGAAACCGCGGAATATCGGCTTAGAGCCTGCCATGGCGAATCGGCTACAGCGCGGGG
ACCGCAGTCGGCTGCTGCTGCTGCTGTGCATTTTCCTGGGGACGCTGCGGGGGTTCCGGG
CCAGGCAGATCCGATATTCGGTGCCAGAAGAGACCGAAAAGGGCTCCTTCGTGGGCAATA
TCTCCAAGGACCTGGGGCTGGAGCCCCGGGAGCTGGCGAAGCGCGGAGTCCGCATCGTCT
CCAGAGGGAAGACACAGCTTTTCGCTGTGAATCCGCGAAGCGGCAGCTTGATCACGGCAG
GCAGGATAGACCGGGAGGAGCTCTGTGAGACGGTGTCCTCCTGTTTTTTAAATATGGAAC
TTCTCGTGGAAGACACCTTGAAGATTTACGGAGTGGAGGTGGAAATAATAGATATTAATG
ATAACGCCCCCAGCTTCCAGGAGGACGAAGTGGAGATAAAAGTCAGTGAGCACGCAATTC
CTGGGGCGCGATTTGCTCTTCCTAATGCTAGGGATCCAGATGTGGGCGTGAACTCCCTCC
AGAGCTACCAGCTCAGCCCTAATAATTACTTTTCCTTGCAACTGCGGGGCAGAACGGATG
GGGCCAAGATCCAGAGCTAGTACTGGNAGGGAAGCCTGGACCGAGAGAANAGAGCTGCTC
ACCTGCTCCTCCTCCAGCTNTAGATGGGGAGCGATCCATTCAGGGGGGCGCAGTCCATCG
NGGGGGGGGGGGGCCTCGATGGAATGATCACTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032092 unedited
AGTTTTAAGTTTNTTATACCCTTCGTCTCGAGNAAAAAAAGCCTCTAAATATTACAGTAT
AAAACTTGCTGTGAGACACAGGAAGAGGGTGGGGTGGGAGCTACGTGACTATGCGAAGAG
AAGGGGGGATAGGAGAAGTCAGTACGGGGGAGGGGGTCGCCTTAGCGCGTGGACACAGCA
CAAAACACAACACGGGCCGCCGCGGGCACATCAAGGACAGACTGTTGCGCGGAGCCCGCC
GTCCGCTGGCCGCGCGGCATTACGTTAAAGCACACCGCACTACACTACTGGCTATTCTAC
ACCAGCCGAAAGAGGGAGGGGGCGCTACACTACTGTAAGGGCAGGTCTGGGGTTCTCTGG
CAGCCCCGGTGGATGGTCGTCGACCCAGGGGCATAACGCGGTGGGCGAAGATAGTCTATG
CACCAGGAGGCACTGGCGAACTTTAACCAGCCTTGGCCAATATGGGGCGCACTGGNCACC
TGGGCCGTGGCCTTCACGGCATACAAACTGCTCCTGGCTCGTCTAACGACTGGGGCTTCC
GCAGGGCATGTGCCCCTGTTGATCAATTCATGTCTTGGGTTGATGACTTTCGCTCCTTTC
CTTCCTGTTTTGTCTACCGTCCTTTCGTTCCTCTGTCCTTTTAAGTGGCGTGGCAGGGCA
TCCTCTCTCTGCCGCCGNTTCACCTTTCGCTTATTCCGCAGCCTTTTTCTCCCTCTCCAG
ACCTTTTCAGTGACACTTTCGGGCGCTTGTTGGCTCCCGTCTCTTAACCCGCATTACGCG
TTGACTATGCCTACATTCGCTCGTTTGTCCTTCCGACCCCATGATATGCTCCCCACGATC
GGCTGTAGCGACCGTTTCCCCATACACCCGCTCGCATAGTTGTCCTCACATACCTTGCTC
CCCGTTCTGCGTTCAGCGCATCGCTCTNGTCCGTCATACGACACTCGATCCACTCCTAAA
GCTCCTAT
Restriction Sites NotI-NotI |
ACCN NM_032092
Insert Size 4260 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032092.1, NP_114481.1
RefSeq Size 4317 bp
RefSeq ORF 2253 bp
Locus ID 56105
UniProt ID Q9Y5H2
Cytogenetics 5q31.3
Protein Families Transmembrane
Summary This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. provided by RefSeq, Jul 2008
Transcript Variant: This variant (3) utilizes an alternative splice donor site that truncates exon 1. This shortest isoform (3) lacks the transmembrane region and has five complete and one partial cadherin domain.
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Documents
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"NM_032092" in other vectors (4)
SKU Description Size Price
RC224531 PCDHGA11 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3 10 ug
$755.00
RC224531L3 Lenti-ORF clone of PCDHGA11 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3 10 ug
$1,055.00
RC224531L4 Lenti-ORF clone of PCDHGA11 (mGFP-tagged)-Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3 10 ug
$1,055.00
RG224531 PCDHGA11 (tGFP-tagged) - Human protocadherin gamma subfamily A, 11 (PCDHGA11), transcript variant 3 10 ug
$955.00

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