DCBLD2 (NM_080927) Human Tagged ORF Clone
$710.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DCBLD2 |
| Synonyms | CLCP1; ESDN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_080927 |
| ORF Size | 2325 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_080927.1 |
| RefSeq Size | 6093 bp |
| RefSeq ORF | 2328 bp |
| Locus ID | 131566 |
| UniProt ID | Q96PD2 |
| Cytogenetics | 3 |
| Domains | CUB, F5_F8_type_C, LCCL |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 84.9 kDa |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_080927" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC224483L1 | Lenti ORF clone of Human discoidin, CUB and LCCL domain containing 2 (DCBLD2), Myc-DDK-tagged | 10 ug |
$1,010.00
|
|
| RC224483L2 | Lenti ORF clone of Human discoidin, CUB and LCCL domain containing 2 (DCBLD2), mGFP tagged | 10 ug |
$1,010.00
|
|
| RC224483L3 | Lenti ORF clone of Human discoidin, CUB and LCCL domain containing 2 (DCBLD2), Myc-DDK-tagged | 10 ug |
$1,010.00
|
|
| RC224483L4 | Lenti ORF clone of Human discoidin, CUB and LCCL domain containing 2 (DCBLD2), mGFP tagged | 10 ug |
$1,010.00
|
|
| RG224483 | DCBLD2 (tGFP-tagged) - Human discoidin, CUB and LCCL domain containing 2 (DCBLD2) | 10 ug |
$789.00
$910.00
|
|
| SC128193 | DCBLD2 (untagged)-Human discoidin, CUB and LCCL domain containing 2 (DCBLD2) | 10 ug |
$711.00
|
|
Citations
FYN and ABL regulate the interaction networks of the DCBLD receptor family
Schmoker, Weinert, Markwood et al
Mol. Cell Proteomics (2020)
Schmoker, Weinert, Markwood et al
Mol. Cell Proteomics (2020)
Dynamic multi-site phosphorylation by Fyn and Abl drives the interaction between CRKL and the novel scaffolding receptors DCBLD1 and DCBLD2
Schmoker, Weinert, Kellett et al
Biochem. J. (2017)
Schmoker, Weinert, Kellett et al
Biochem. J. (2017)
AI Summary: OriGene contributed to this study by providing mammalian expression constructs for full-length DCBLD2 (Hs) (#RC224483) and DCBLD1 (Mm) (#MR206887), which were essential for investigating the phosphorylation-dependent interactions between these receptors and CRKL.
Snippet: Plasmids Mammalian expression constructs for full-length DCBLD2 (Hs) and DCBLD1 (Mm) in pCMV6- Entry, tagged with Flag and Myc sequences at C-termini, were obtained from Origene Page 7. (#RC224483 and #MR206887 respectively;RocNville, MD, USA). ...;... Plasmids Mammalian expression constructs for full-length DCBLD2 (Hs) and DCBLD1 (Mm) in pCMV6- Entry, tagged with Flag and Myc sequences at C-termini, were obtained from Origene Page 7. (#RC224483 and #MR206887 respectively;RocNville, MD, USA).
Tyrosine phosphorylation of the orphan receptor ESDN/DCBLD2 serves as a scaffold for the signaling adaptor CrkL
Aten, Redmond, Weaver et al
FEBS Lett. (2013) 587 (15), 2313-8 DOI: 10.1016/j.febslet.2013.05.064
Aten, Redmond, Weaver et al
FEBS Lett. (2013) 587 (15), 2313-8 DOI: 10.1016/j.febslet.2013.05.064
Cited by 24
AI Summary: OriGene contributed to this study by providing the expression construct for full-length human ESDN with Myc and Flag (DDK) tags (product #RC224483), which was used to investigate ESDN's role in tyrosine phosphorylation and its interaction with signaling proteins.
Snippet: 2.1. Plasmids and site-directed mutagenesis. The expression construct for full-length human ESDN with Myc and Flag (DDK) tags at the carboxyl-terminus in pCMV6-Entry was obtained from Origene (product #RC224483;Rockville, MD). ... Indicates non-specific band.
Showing 1-3 of 3 papers.
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