PGC1 beta (PPARGC1B) (NM_133263) Human Tagged ORF Clone
SKU
RC224480
PPARGC1B (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1
$891.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PGC1 beta |
| Synonyms | ERRL1; PERC; PGC-1(beta); PGC1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_133263 |
| ORF Size | 3069 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_133263.2, NP_573570.2 |
| RefSeq Size | 3277 bp |
| RefSeq ORF | 3072 bp |
| Locus ID | 133522 |
| UniProt ID | Q86YN6 |
| Cytogenetics | 5q32 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 113 kDa |
| Summary | The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_133263" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC224480L1 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,191.00
|
|
| RC224480L2 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1, mGFP tagged | 10 ug |
$1,191.00
|
|
| RC224480L3 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,191.00
|
|
| RC224480L4 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1, mGFP tagged | 10 ug |
$1,191.00
|
|
| RG224480 | PPARGC1B (tGFP-tagged) - Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1 | 10 ug |
$1,091.00
|
|
| SC305937 | PPARGC1B (untagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 1 | 10 ug |
$891.00
|
|
Citations
Genetic variants of PPAR-gamma coactivator 1B augment NLRP3-mediated inflammation in gouty arthritis
Chang, Wu, Chung et al
Rheumatology (2016)
Chang, Wu, Chung et al
Rheumatology (2016)
AI Summary: OriGene contributed to this study by providing the Myc-DDK-tagged hPPARGC1B plasmid, which was used for transfection and siRNA knockdown experiments investigating the role of PPARGC1B in NLRP3-mediated inflammation in gouty arthritis.
Snippet: The experiments were done in triplicate. Transfection and siRNA knockdownof PPARGC1B. The plasmid hPPARGC1B (Myc-DDK-tagged) containing full-length cDNAof PPARGC1B was obtained from Origene (Rockville, MD, USA). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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