DERA (NM_015954) Human Tagged ORF Clone

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SKU
RC224461
DERA (Myc-DDK-tagged)-Human deoxyribose-phosphate aldolase (putative) (DERA)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DERA
Synonyms CGI-26; DEOC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224461 representing NM_015954
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGCGCACAATCGGGGCACCGAGCTCGACCTTAGCTGGATCTCCAAAATACAAGTGAATCACCCGG
CAGTTCTGAGGCGTGCGGAACAAATCCAGGCTCGCAGAACCGTGAAAAAGGAGTGGCAGGCTGCTTGGCT
CCTGAAAGCTGTTACCTTTATAGATCTTACTACACTTTCAGGTGATGATACATCTTCCAACATTCAAAGG
CTCTGTTATAAAGCCAAATACCCAATCCGGGAAGATCTCTTAAAAGCTTTAAATATGCATGATAAAGGCA
TTACTACAGCCGCCGTTTGTGTTTATCCCGCCCGGGTGTGTGATGCTGTAAAAGCACTCAAGGCTGCAGG
CTGTAATATCCCTGTGGCATCAGTGGCCGCTGGATTTCCAGCTGGACAGACTCATTTGAAGACACGATTA
GAAGAGATCAGATTGGCTGTGGAAGATGGAGCTACAGAAATCGACGTGGTAATTAACAGAAGCTTGGTGC
TGACAGGCCAGTGGGAAGCCCTGTATGATGAGATTCGTCAGTTTCGCAAGGCCTGTGGGGAGGCTCATCT
TAAAACTATATTAGCGACAGGAGAACTTGGAACTCTTACTAATGTCTATAAAGCCAGTATGATAGCAATG
ATGGCAGGATCAGATTTTATTAAGACCTCTACTGGAAAAGAAACAGTAAATGCCACCTTCCCGGTAGCTA
TAGTAATGCTGCGGGCCATTAGAGATTTCTTCTGGAAAACTGGAAACAAGATAGGGTTTAAACCAGCAGG
AGGCATCCGCAGTGCAAAGGATTCCCTTGCTTGGCTCTCTCTTGTAAAGGAGGAGCTTGGAGATGAGTGG
CTGAAGCCAGAACTCTTTCGAATAGGTGCCAGTACTCTGCTCTCGGACATTGAGAGGCAGATTTACCATC
ATGTGACTGGAAGATATGCAGCTTATCATGATCTTCCAATGTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC224461 representing NM_015954
Red=Cloning site Green=Tags(s)

MSAHNRGTELDLSWISKIQVNHPAVLRRAEQIQARRTVKKEWQAAWLLKAVTFIDLTTLSGDDTSSNIQR
LCYKAKYPIREDLLKALNMHDKGITTAAVCVYPARVCDAVKALKAAGCNIPVASVAAGFPAGQTHLKTRL
EEIRLAVEDGATEIDVVINRSLVLTGQWEALYDEIRQFRKACGEAHLKTILATGELGTLTNVYKASMIAM
MAGSDFIKTSTGKETVNATFPVAIVMLRAIRDFFWKTGNKIGFKPAGGIRSAKDSLAWLSLVKEELGDEW
LKPELFRIGASTLLSDIERQIYHHVTGRYAAYHDLPMS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015954
ORF Size 954 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015954.4
RefSeq Size 1561 bp
RefSeq ORF 957 bp
Locus ID 51071
UniProt ID Q9Y315
Cytogenetics 12p12.3
Protein Families Druggable Genome
Protein Pathways Pentose phosphate pathway
MW 35.1 kDa
Summary Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate. Participates in stress granule (SG) assembly. May allow ATP production from extracellular deoxyinosine in conditions of energy deprivation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_015954" in other vectors (6)
SKU Description Size Price
RC224461L1 Lenti ORF clone of Human deoxyribose-phosphate aldolase (putative) (DERA), Myc-DDK-tagged 10 ug
$600.00
RC224461L2 Lenti ORF clone of Human deoxyribose-phosphate aldolase (putative) (DERA), mGFP tagged 10 ug
$600.00
RC224461L3 Lenti ORF clone of Human deoxyribose-phosphate aldolase (putative) (DERA), Myc-DDK-tagged 10 ug
$600.00
RC224461L4 Lenti ORF clone of Human deoxyribose-phosphate aldolase (putative) (DERA), mGFP tagged 10 ug
$600.00
RG224461 DERA (tGFP-tagged) - Human deoxyribose-phosphate aldolase (putative) (DERA) 10 ug
$500.00
SC304371 DERA (untagged)-Human deoxyribose-phosphate aldolase (putative) (DERA) 10 ug
$300.00

Plasmid Map for RC224461

Download sequence for

Cloning scheme for RC224461

Circular map for RC224461

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