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CHCHD7 (NM_024300) Human Tagged ORF Clone

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SKU
RC224325
CHCHD7 (Myc-DDK-tagged)-Human coiled-coil-helix-coiled-coil-helix domain containing 7 (CHCHD7), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "CHCHD7 or NM_024300"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CHCHD7
Synonyms COX23
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224325 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACCAAACTCGAACTGGTAAGAAGACTGTTAGAATGCCCTCGGTAACACAGAGGCTGAGAGATCCTG
ACATAAATCCTTGTTTGTCGGAATCTGATGCTTCCACCAGATGTCTGGATGAAAATAACTATGACAGGGA
AAGGTGTTCCACTTACTTCTTGAGGTACAAAAACTGCCGGAGATTCTGGAATTCTATCGTGATGCAGAGA
AGAAAGAACGGAGTGAAGCCATTTATGCCTACGGCAGCAGAAAGAGATGAAATCTTGAGAGCAGTGGGAA
ATATGCCCTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224325 protein sequence
Red=Cloning site Green=Tags(s)

MHQTRTGKKTVRMPSVTQRLRDPDINPCLSESDASTRCLDENNYDRERCSTYFLRYKNCRRFWNSIVMQR
RKNGVKPFMPTAAERDEILRAVGNMPY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024300
ORF Size 291 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024300.4
RefSeq Size 1703 bp
RefSeq ORF 294 bp
Locus ID 79145
UniProt ID Q9BUK0
Cytogenetics 8q12.1
MW 11.5 kDa
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"NM_024300" in other vectors (4)
SKU Description Size Price
RC224325L3 Lenti ORF clone of Human coiled-coil-helix-coiled-coil-helix domain containing 7 (CHCHD7), transcript variant 4, Myc-DDK-tagged 10 ug
$450.00
RC224325L4 Lenti ORF clone of Human coiled-coil-helix-coiled-coil-helix domain containing 7 (CHCHD7), transcript variant 4, mGFP tagged 10 ug
$450.00
RG224325 CHCHD7 (tGFP-tagged) - Human coiled-coil-helix-coiled-coil-helix domain containing 7 (CHCHD7), transcript variant 4 10 ug
$489.00
SC112264 CHCHD7 (untagged)-Human coiled-coil-helix-coiled-coil-helix domain containing 7 (CHCHD7), transcript variant 4 10 ug
$150.00

Plasmid Map for RC224325

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224325
Circular map for RC224325

file-alt Citations

SUMO1 degrader induces ER stress and ROS accumulation through deSUMOylation of TCF4 and inhibition of its transcription of StarD7 in colon cancer
Zhao, Jin, Kim et al
Mol Carcinog (2023)
Cited by 15
AI Summary: OriGene products pCMV6-Entry (Myc-Flag-tagged, CAT# PS100001) and TCF4 (Myc-Flag-tagged, CAT# RC224325) plasmids were used to express tagged proteins for mechanistic studies of SUMO1 degrader effects in colon cancer cells.
Snippet: Human colorectal cancer cell lines HCT116 and DLD-1 were purchased from the American Type Culture Collection (ATCC) and grown in DMEM (HCT116) and RPMI 1640 medium (DLD1), supplemented with 10% FBS (Corning) as previously reported.1 YFP-SUMO1-GV mutants were generated as described8;pCMV6-Entry (Myc-Flag-tagged) and TCF4 (Myc-Flag-tagged) were purchased from Origene (CAT# PS100001;CAT# RC224325)
Showing 1-1 of 1 papers.
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