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MRPL21 (NM_181514) Human Tagged ORF Clone

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SKU
RC224225
MRPL21 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L21 (MRPL21), nuclear gene encoding mitochondrial protein, transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MRPL21
Synonyms L21mt; MRP-L21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224225 representing NM_181514
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCATCTTCCCTGACGGTCACCTTAGGGCGGCTGGCGTCCGCGTGCAGCCACAGCATCCTGAGAC
CTTCGGGGCCCGGAGCAGCCTCCCTTTGGTCTGCTTCTCGAAGGTTCAATTCACAGAGCACTTCATATCT
ACCAGGATATGTTCCTAAAACATCCCTGAGTTCACCACCTTGGCCAGAAGTTGTTCTGCCAGACCCAGTT
GAGGAGACCAGACACCATGCAGAGGTCGTGAAGAAGGTGAATGAGATGATCGTCACGGGGCAGTATGGCA
GGCTCTTTGCCGTGGTGCACTTTGCCAGCCGCCAGTGGAAGGTGACCTCTGAAGACCTGATCTTAATTGG
AAATGAACTAGACCTTGCGTGTGGAGAGAGAATTCGACTGGAGAAGGTCCTGCTGGTTGGGGCAGACAAC
TTCACGCTGCTTGGCAAGCCACTCCTCGGAAAGGATCTTGTTCGAGTAGAAGCCACAGTCATTGAAAAGA
CAGAATCATGGCCAAGAATCATTATGAGATTCAGGAAAAGGAAAAACTTCAAGAAGAAAAGAATCGTCAC
GACCCCGCAGACTGTCCTCCGGATAAACAGCATTGAGATTGCTCCGTGTTTGTTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224225 representing NM_181514
Red=Cloning site Green=Tags(s)

MAASSLTVTLGRLASACSHSILRPSGPGAASLWSASRRFNSQSTSYLPGYVPKTSLSSPPWPEVVLPDPV
EETRHHAEVVKKVNEMIVTGQYGRLFAVVHFASRQWKVTSEDLILIGNELDLACGERIRLEKVLLVGADN
FTLLGKPLLGKDLVRVEATVIEKTESWPRIIMRFRKRKNFKKKRIVTTPQTVLRINSIEIAPCLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181514
ORF Size 615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181514.2
RefSeq Size 713 bp
RefSeq ORF 618 bp
Locus ID 219927
UniProt ID Q7Z2W9
Cytogenetics 11q13.3
MW 22.6 kDa
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD). provided by RefSeq, Jul 2008
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Documents
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"NM_181514" in other vectors (4)
SKU Description Size Price
RC224225L3 Lenti ORF clone of Human mitochondrial ribosomal protein L21 (MRPL21), nuclear gene encoding mitochondrial protein, transcript variant 4, Myc-DDK-tagged 10 ug
$600.00
RC224225L4 Lenti ORF clone of Human mitochondrial ribosomal protein L21 (MRPL21), nuclear gene encoding mitochondrial protein, transcript variant 4, mGFP tagged 10 ug
$600.00
RG224225 MRPL21 (tGFP-tagged) - Human mitochondrial ribosomal protein L21 (MRPL21), nuclear gene encoding mitochondrial protein, transcript variant 4 10 ug
$489.00 $500.00
SC107293 MRPL21 (untagged)-Human mitochondrial ribosomal protein L21 (MRPL21), nuclear gene encoding mitochondrial protein, transcript variant 4 10 ug
$300.00

Plasmid Map for RC224225

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224225
Circular map for RC224225

file-alt Citations

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