MRPL21 (NM_181514) Human Untagged Clone

SKU SC107293

MRPL21 (untagged)-Human mitochondrial ribosomal protein L21 (MRPL21), nuclear gene encoding mitochondrial protein, transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: MRPL21
• Synonyms: L21mt; MRP-L21
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107293 sequence for NM_181514 edited (data generated by NextGen Sequencing); ATGGCAGCATCTTCCCTGACGGTCACCTTAGGGCGGCTGGCGTCCGCGTGCAGCCACAGC; ATCCTGAGACCTTCGGGGCCCGGAGCAGCCTCCCTTTGGTCTGCTTCTCGAAGGTTCAAT; TCACAGAGCACTTCATATCTACCAGGATATGTTCCTAAAACATCCCTGAGTTCACCACCT; TGGCCAGAAGTTGTTCTGCCAGACCCAGTTGAGGAGACCAGACACCATGCAGAGGTCGTG; AAGAAGGTGAATGAGATGATCGTCACGGGGCAGTATGGCAGGCTCTTTGCCGTGGTGCAC; TTTGCCAGCCGCCAGTGGAAGGTGACCTCTGAAGACCTGATCTTAATTGGAAATGAACTA; GACCTTGCGTGTGGAGAGAGAATTCGACTGGAGAAGGTCCTGCTGGTTGGGGCAGACAAC; TTCACGCTGCTTGGCAAGCCACTCCTCGGAAAGGATCTTGTTCGAGTAGAAGCCACAGTC; ATTGAAAAGACAGAATCATGGCCAAGAATCATTATGAGATTCAGGAAAAGGAAAAACTTC; AAGAAGAAAAGAATCGTCACGACCCCGCAGACTGTCCTCCGGATAAACAGCATTGAGATT; GCTCCGTGTTTGTTGTGA; ; Clone variation with respect to NM_181514.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181514 unedited; ACGAGGCGGCGGCCATGGCAGCATCTTCCCTGNACGGTCACCTTAGGGCGGCTGGCGTCC; GCGTGCAGCCACAGCATCCTGAGACCTTCGGGGCCCGGAGCAGCCTCCCTTTGGTCTGCT; TCTCGAAGGTTCAATTCACAGAGCACTTCATATCTACCAGGATATGTTCCTAAAACATCC; CTGAGTTCACCACCTTGGCCAGAAGTTGTTCTGCCAGACCCAGTTGAGGAGACCAGACAC; CATGCAGAGGTCGTGAAGAAGGTGAATGAGATGATCGTCACGGGGCAGTATGGCAGGCTC; TTTGCCGTGGTGCACTTTGCCAGCCGCCAGTGGAAGGTGACCTCTGAAGACCTGATCTTA; ATTGGAAATGAACTAGACCTTGCGTGTGGAGAGAGAATTCGACTGGAGAAGGTCCTGCTG; GTTGGGGCAGACAACTTCACGCTGCTTGGCAAGCCACTCCTCGGAAAGGATCTTGTTCGA; GTAGAAGCCACAGTCATTGAAAAGACAGAATCATGGCCAAGAATCATTATGAGATTCAGG; AAAAGGAAAAACTTCAAGAAGAAAAGAATCGTCACGACCCCGCAGACTGTCCTCCGGATA; AACAGCATTGAGATTGCTCCGTGTTTGTTGTGATTACCGAGTTAATACTTACAAAAGGAT; AAAAATAAACTCCTGCTTNCCAAGGAANNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNN; NNNAAANNAAAAACTCGACTNTAAATTGNGGCCGNGGTCATAACTTGNTTNCTGAAACAA; NNCCCGGGNGGCATCCCTGGGACCCCTCCCAAGGGCCTTTTCTGGCCCTGGAAGTTGCCC; TCCAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_181514 unedited; AACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTCCCTGGGGAAGCAGGAGTTTATTTTTATCCTTTTGGAAGTATTAAC; TCGGTAATCACAACAAACACGGAGCAATCTCAATGCTGTTTATCCGGAGGACAGTCTGCG; GGGTCGTGACAATTCTTTTCTTCTTGAAGTTTTTCCTTTTCCTGAATCTCATAATGATTC; TTGGCCATGATTCTGTCTTTTCAATGACTGGGGCTTCTACTCGAACAAAATCCTTTCCGA; GGAGTGGCTTGCCAAGCAGCGTGAAGTTGTCTGCCCCAACCAGCAGGACCTTTTCCAGTC; AAATTCTCTCTCCACACCCAAGGGCTAGTTCATTTCCAATTAAAATCAGGTCTTCAAAGG; TCACCTTCCACTGGCGGCTGGCAAAGTGCACCACGGCAAAAAGCCTGCCATACTGCCCCG; TGACGATCATCTCATTCACCTTCTTCACGACCTCTGCATGGTGTCTGGTCTCCTCAACTG; GGTCTGGCAGAACAACTTCTGGCCAAGGTGGTGAACTCAGGGATGTTTTAGGAACATATC; CTGGTAGATATGAAGTGCTCTGTGAATTGAACCTTCGAGAGCAGACCAAAGGGAGGCTGC; TCCGGGCCCCCGAGGTCTCAGGATGCTGTGGCTGCACGCGGACCCCAGCCGCCCTAAGTG; ACCGTCACGGAAAATCTGCATGGCCGCCCCTCGTGCCAATTCGCGGCCGCCCATGGGAGT; CGTATAACAAATTCTGACGTTCACTAAACGACCTCTGTTATTATGACTCCCCCGTAACCG; CCTACGGCCATTTGGTAACGGGGCGGGGTATTACCAATTTTGGAAGATCCCTTGATTTGG; GGCAAACAACTTCCTTGACTCATAGGGGGGAACTGGAAAACCGGGGATCAACCCTTTCCC; GCCCTTGGGGTCTGGCAAACCGCTACCCTGTAAAACGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_181514
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_181514.1, NP_852615.1
• RefSeq Size: 713 bp
• RefSeq ORF: 618 bp
• Locus ID: 219927
• UniProt ID: Q7Z2W9
• Cytogenetics: 11q13.3
• Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD). [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (4) encodes the longest protein (isoform d).