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MAP7D3 (NM_024597) Human Tagged ORF Clone

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SKU
RC224223
MAP7D3 (Myc-DDK-tagged)-Human MAP7 domain containing 3 (MAP7D3), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $802.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAP7D3
Synonyms MDP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224223 representing NM_024597
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGCGGACGGCGCCGCAGCTGGCGCTGGCGGCAGCCCATCCTTGAGAGAGCTGCGGGCACGGATGG
TTGCTGCAGCAAACGAGATTGCTAAGGAAAGGAGGAAGCAAGATGTGGTTAATCGTGTTGCAACCCATTC
CTCAAATATAAGATCGACATTTAAACCAGTAATCGATGGATCCATGCTTAAAAATGACATAAAACAAAGA
TTAGCAAGAGAGCGCAGAGAGGAGAAAAGGAGACAGCAAGACGCCAATAAAGAAACACAACTACTTGAAA
AAGAAAGAAAGACCAAGCTCCAATATGAAAAACAGATGGAGGAAAGACAGAGAAAGCTGAAGGAGCGAAA
AGAGAAAGAAGAACAACGGAGAATAGCTGCAGAAGAAAAAAGACACCAGAAGGATGAAGCACAAAAGGAA
AAATTTACAGCCATTCTTTATCGTACTTTGGAACGGAGGAGACTTGCTGATGATTATCAGCAAAAAAGAT
GGTCATGGGGAGGCTCTGCAATGGCGAATTCTGAGAGCAAAACTGCCAATAAACGATCTGCATCTACTGA
AAAACTTGAACAGGGTACTTCTGCTTTAATCAGACAAATGCCTTTGTCATCTGCAGGCCTTCAAAATTCC
GTTGCCAAAAGGAAAACAGACAAGGAGAGAAGCTCATCTTTAAATAGAAGAGATAGTAACCTACATTCGT
CTACTGATAAAGAACAAGCCGAAAGGAAGCCACGTGTTACAGGCGTCACCAATTATGTAATGCAGTATGT
CACTGTACCCTTGCGTAAATGTACTAGCGACGAATTGAGGGCTGTTATGTTTCCCATGTCGACAATGAAA
ATACCTCCTCAAACAAAAGTAGAAGAGTCTCCCTTGGAGAAAGTAGAGACACCTCCCAAGGCAAGTGTGG
ATGCACCCCCCCAGGTGAATGTGGAAGTATTCTGCAACACAAGCATGGAAGCGTCCCCCAAGGCAGGTGT
GGGCATGGCCCCTGAGGTGAGCACGGACTCATTCCCTGTGGTGAGCGTGGACGTGTCGCCTGTGGTGAGC
ACATATGATTCTGAGATGAGCATGGACGCATCCCCCGAGTTGAGCATAGAAGCACTCCCGAAGGTGGACC
TGGAAACAGTTCCCAAGGTGAGCATAGTAGCATCCCCGGAGGCGAGCCTGGAAGCACCCCCGGAAGTGAG
TCTGGAAGCACTGCCAGAGGTGAGCGTGGAAGCAGCCCCAGAGGGGAGCCTGGAAGCACCTCCCAAGGGG
AGCGCAGAAGTAGCCCCCAAGGAGAGTGTGAAAGGGTCACCCAAGGAGAGCATGGAGGCATCTCCTGAGG
CGATGGTGAAAGCATCCCCCAAGACATCCCTTGAAGCAAGCATGGAAGCATCTCCCAAGGCAAAAGCGAG
AGACGCTCCAAAGAAATCAGAAATGGACAAACAGGCCTTAATCCCTATTGCCAAGAAGCGTCTATCATCA
TACACTGAGTGTTATAAATGGTCATCATCTCCTGAAAATGCTTGTGGTCTGCCGTCTCCCATCAGCACTA
ACAGGCAAATCCAAAAGAACTGCCCTCCATCACCATTACCACTTATTTCAAAACAGTCACCACAGACTTC
TTTTCCTTATAAAATAATGCCTATTCAACACACCCTGTCTGTGCAAAGTGCATCAAGTACTGTCAAAAAG
AAAAAAGAAACAGTTTCTAAAACCACTAACAGATGTGAGGCTTTGAGCCAAAGGCATATGATCTATGAAG
AGTCTGGTAATAAGAGTACTGCAGGTATTATGAATGCCGAGGCGGCAACAAAAATTTTGACAGAATTGCG
CCGCCTTGCTCGTGAACAAAGAGAAAAAGAGGAAGAAGAAAGACAACGGGAAGAAATGCAGCAAAGGGTC
ATTAAGAAATCAAAAGACATGGCAAAGGAAGCAGTTGGAGGCCAAGCAGAAGACCACTTGAAACTCAAAG
ATGGGCAGCAACAAAATGAGACTAAAAAGAAGAAAGGATGGCTGGATCAGGAAGACCAGGAAGCACCACT
GCAGAAAGGGGACGCCAAAATAAAAGCTCAAGAGGAAGCTGACAAACGCAAGAAAGAACACGAGAGAATT
ATGTTACAAAATTTACAAGAACGGTTAGAAAGGAAAAAGAGAATAGAAGAAATTATGAAGCGGACAAGAA
AGACAGATGTGAATGCCTCAAAGGTCACAGAAACATCCAGCCATGACATATATGAAGAGGCTGAGGCTGA
CAACGAAGAAAGCGACAAGGACTCATTGAATGAAATGTTTCCATCAGCCATTCTAAATGGCACAGGCTCA
CCTACCAAATTTAAAATGCCGTTCAACAATGCCAAGAAAATGACACACAAGCTGGTATTTCTAGAAGATG
GTACCAGCCAGGTCCGTAAAGAGCCAAAAACATATTTTAATGGCGATTTGAAAAACTTCAGACAAAAAAG
CATGAAAGACACTTCAATACAGGAAGTAGTTTCAAGACCATCTTCCAAAAGAATGACCAGTCACACAACG
AAAACCAGAAAGGCGGATGAAACCAACACCACCAGCAGATCCTCTGCACAAACAAAATCTGAAGGATTCC
ATGACATCTTGCCAAAGTCCTCAGACACCTTTAGACAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224223 representing NM_024597
Red=Cloning site Green=Tags(s)

MMADGAAAGAGGSPSLRELRARMVAAANEIAKERRKQDVVNRVATHSSNIRSTFKPVIDGSMLKNDIKQR
LARERREEKRRQQDANKETQLLEKERKTKLQYEKQMEERQRKLKERKEKEEQRRIAAEEKRHQKDEAQKE
KFTAILYRTLERRRLADDYQQKRWSWGGSAMANSESKTANKRSASTEKLEQGTSALIRQMPLSSAGLQNS
VAKRKTDKERSSSLNRRDSNLHSSTDKEQAERKPRVTGVTNYVMQYVTVPLRKCTSDELRAVMFPMSTMK
IPPQTKVEESPLEKVETPPKASVDAPPQVNVEVFCNTSMEASPKAGVGMAPEVSTDSFPVVSVDVSPVVS
TYDSEMSMDASPELSIEALPKVDLETVPKVSIVASPEASLEAPPEVSLEALPEVSVEAAPEGSLEAPPKG
SAEVAPKESVKGSPKESMEASPEAMVKASPKTSLEASMEASPKAKARDAPKKSEMDKQALIPIAKKRLSS
YTECYKWSSSPENACGLPSPISTNRQIQKNCPPSPLPLISKQSPQTSFPYKIMPIQHTLSVQSASSTVKK
KKETVSKTTNRCEALSQRHMIYEESGNKSTAGIMNAEAATKILTELRRLAREQREKEEEERQREEMQQRV
IKKSKDMAKEAVGGQAEDHLKLKDGQQQNETKKKKGWLDQEDQEAPLQKGDAKIKAQEEADKRKKEHERI
MLQNLQERLERKKRIEEIMKRTRKTDVNASKVTETSSHDIYEEAEADNEESDKDSLNEMFPSAILNGTGS
PTKFKMPFNNAKKMTHKLVFLEDGTSQVRKEPKTYFNGDLKNFRQKSMKDTSIQEVVSRPSSKRMTSHTT
KTRKADETNTTSRSSAQTKSEGFHDILPKSSDTFRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024597
ORF Size 2628 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024597.4
RefSeq Size 3027 bp
RefSeq ORF 2631 bp
Locus ID 79649
UniProt ID Q8IWC1
Cytogenetics Xq26.3
MW 98.2 kDa
Summary The protein encoded by this gene belongs to the MAP7 (microtubule-associated protein 7) family. Alternatively spliced transcript variants encoding different isoforms have been found. provided by RefSeq, Mar 2010
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Documents
Resources
"NM_024597" in other vectors (4)
SKU Description Size Price
RC224223L3 Lenti ORF clone of Human MAP7 domain containing 3 (MAP7D3), transcript variant 1, Myc-DDK-tagged 10 ug
$1,102.00
RC224223L4 Lenti ORF clone of Human MAP7 domain containing 3 (MAP7D3), transcript variant 1, mGFP tagged 10 ug
$1,102.00
RG224223 MAP7D3 (tGFP-tagged) - Human MAP7 domain containing 3 (MAP7D3), transcript variant 1 10 ug
$789.00 $1,002.00
SC316275 MAP7D3 (untagged)-Human MAP7 domain containing 3 (MAP7D3), transcript variant 1 10 ug
$883.00

Plasmid Map for RC224223

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224223
Circular map for RC224223

file-alt Citations

DDA3 and Mdp3 modulate Kif2a recruitment onto the mitotic spindle to control minus-end spindle dynamics
Kwon, Park, Song et al
J. Cell. Sci. (2016)
Cited by 24
AI Summary: OriGene contributed to this study by providing the full-length Mdp3 gene, which was used to generate tagged constructs for investigating the role of Mdp3 in spindle dynamics.
Snippet: Page 11. Journal of Cell Science • Advance article Materials and Methods Plasmids and Antibodies The full-length Mdp3 gene was purchased from OriGene (UAS) and subcloned into the pCS2+ vector with an N-terminal GFP or Myc tag. ... Related articles All 2 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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