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ELF2 (NM_201999) Human Tagged ORF Clone

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SKU
RC224036
ELF2 (Myc-DDK-tagged)-Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $541.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ELF2
Synonyms b; EU32; NERF; NERF-1A; NERF-1a; NERF-1B; NERF-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224036 representing NM_201999
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACATCAGCAGTGGTTGACAGTGGAGGTACTATTTTGGAGCTTTCCAGCAATGGAGTAGAAAATCAAG
AGGAAAGTGAAAAGGTTTCTGAATATCCAGCAGTGATTGTGGAGCCAGTTCCAAGTGCCAGATTAGAGCA
GGGCTATGCAGCCCAGGTTCTGGTTTATGATGATGAGACTTATATGATGCAAGATGTGGCAGAAGAACAA
GAAGTTGAGACCGAGAATGTGGAAACAGTGGAAGCATCAGTTCACAGCAGTAATGCACACTGTACAGATA
AGACAATTGAAGCTGCTGAAGCCCTGCTTCATATGGAATCTCCTACCTGCTTGAGGGATTCAAGAAGTCC
TGAATTCATCCATGCTGCTATGAGGCCAGATGTCATTACAGAAACTGTAGTGGAGGTGTCAACTGAAGAG
TCTGAACCCATGGATACCTCTCCTATTCCAACATCACCAGATAGCCATGAACCAATGAAAAAGAAAAAAG
TTGGCCGTAAACCAAAGACCCAGCAATCACCAATTTCCAATGGGTCTCCTGAGTTAGGTATAAAGAAGAA
ACCAAGAGAAGGAAAAGGAAACACAACCTATTTGTGGGAGTTTCTTTTAGATCTACTTCAAGATAAAAAT
ACTTGTCCCAGGTATATTAAATGGACTCAGAGAGAAAAAGGCATATTCAAGCTGGTGGATTCAAAGGCTG
TCTCTAAGCTTTGGGGAAAGCATAAGAACAAACCAGACATGAACTATGAAACCATGGGACGAGCTTTGAG
ATACTACTACCAAAGGGGAATTCTTGCAAAGGTTGAAGGACAGAGGCTTGTATATCAGTTCAAGGATATG
CCGAAAAACATAGTGGTCATAGATGATGACAAAAGTGAAACCTGTAATGAAGATTTAGCAGGAACTACTG
ATGAAAAATCATTAGAACGAGTGTCACTGTCTGCAGAAAGTCTCCTGAAAGCAGCATCCTCTGTTCGCAG
TGGAAAAAATTCATCCCCTATAAACTGCTCCAGAGCAGAGAAGGGTGTAGCTAGAGTTGTGAATATCACT
TCCCCTGGGCACGATGCTTCATCCAGGTCTCCTACTACCACTGCATCTGTGTCAGCAACAGCAGCTCCAA
GGACAGTTCGTGTGGCAATGCAGGTACCTGTTGTAATGACATCATTGGGTCAGAAAATTTCAACTGTGGC
AGTTCAGTCAGTTAATGCAGGTGCACCATTAATAACCAGCACTAGTCCAACAACAGCGACCTCTCCAAAG
GTAGTCATTCAGACAATCCCTACTGTGATGCCAGCTTCTACTGAAAATGGAGACAAAATCACCATGCAGC
CTGCCAAAATTATTACCATCCCAGCTACACAGCTTGCACAGTGTCAACTGCAGACAAAGTCAAATCTGAC
TGGATCAGGAAGCATTAACATTGTTGGAACCCCATTGGCTGTGAGAGCACTTACCCCTGTTTCAATAGCC
CATGGTACACCTGTAATGAGACTATCAATGCCTACTCAGCAGGCATCTGGCCAGACTCCTCCTCGAGTTA
TCAGTGCAGTCATAAAGGGGCCAGAGGTTAAATCGGAAGCAGTGGCAAAAAAGCAAGAACATGATGTGAA
AACTTTGCAGCTAGTAGAAGAAAAACCAGCAGATGGAAATAAGACAGTGACCCACGTAGTGGTTGTCAGT
GCGCCTTCAGCTATTGCCCTTCCTGTAACTATGAAAACAGAAGGACTAGTGACATGTGAGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224036 representing NM_201999
Red=Cloning site Green=Tags(s)

MTSAVVDSGGTILELSSNGVENQEESEKVSEYPAVIVEPVPSARLEQGYAAQVLVYDDETYMMQDVAEEQ
EVETENVETVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPEFIHAAMRPDVITETVVEVSTEE
SEPMDTSPIPTSPDSHEPMKKKKVGRKPKTQQSPISNGSPELGIKKKPREGKGNTTYLWEFLLDLLQDKN
TCPRYIKWTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKDM
PKNIVVIDDDKSETCNEDLAGTTDEKSLERVSLSAESLLKAASSVRSGKNSSPINCSRAEKGVARVVNIT
SPGHDASSRSPTTTASVSATAAPRTVRVAMQVPVVMTSLGQKISTVAVQSVNAGAPLITSTSPTTATSPK
VVIQTIPTVMPASTENGDKITMQPAKIITIPATQLAQCQLQTKSNLTGSGSINIVGTPLAVRALTPVSIA
HGTPVMRLSMPTQQASGQTPPRVISAVIKGPEVKSEAVAKKQEHDVKTLQLVEEKPADGNKTVTHVVVVS
APSAIALPVTMKTEGLVTCEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201999
ORF Size 1743 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201999.3
RefSeq Size 3218 bp
RefSeq ORF 1746 bp
Locus ID 1998
UniProt ID Q15723
Cytogenetics 4q31.1
Protein Families Druggable Genome, Transcription Factors
MW 62.5 kDa
Summary Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_201999" in other vectors (6)
SKU Description Size Price
RC224036L1 Lenti ORF clone of Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 1, Myc-DDK-tagged 10 ug
$841.00
RC224036L2 Lenti ORF clone of Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 1, mGFP tagged 10 ug
$841.00
RC224036L3 Lenti ORF clone of Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 1, Myc-DDK-tagged 10 ug
$841.00
RC224036L4 Lenti ORF clone of Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 1, mGFP tagged 10 ug
$841.00
RG224036 ELF2 (tGFP-tagged) - Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 1 10 ug
$489.00 $741.00
SC121455 ELF2 (untagged)-Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 1 10 ug
$542.00

Plasmid Map for RC224036

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224036
Circular map for RC224036

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