OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

ALG10 (NM_032834) Human Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RC223859
ALG10 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog (S. pombe) (ALG10)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA331308 Rabbit Polyclonal Anti-ALG10 Antibody
100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "ALG10 or NM_032834"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ALG10
Synonyms ALG10A; DIE2; KCR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223859 representing NM_032834
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCAGCTGGAAGGTTACTATTTCTCGGCCGCCTTGAGCTGTACCTTTTTAGTATCCTGCCTCCTCT
TCTCCGCCTTCAGCCGGGCGTTGCGAGAGCCCTACATGGACGAGATCTTCCACCTGCCTCAGGCGCAGCG
CTACTGTGAGGGCCATTTCTCCCTTTCCCAGTGGGATCCCATGATTACTACATTACCTGGCTTGTACCTG
GTGTCAATTGGAGTGATCAAACCTGCCATTTGGATCTTTGGATGGTCTGAACATGTTGTCTGCTCCATTG
GGATGCTCAGATTTGTTAATCTTCTCTTCAGTGTTGGCAACTTCTATTTACTATATTTGCTTTTCTGCAA
GGTACAACCCAGAAACAAGGCTGCCTCAAGTATCCAGAGAGTCTTGTCAACATTAACACTAGCAGTATTT
CCAACACTTTATTTTTTTAACTTCCTTTATTATACAGAAGCAGGATCTATGTTTTTTACTCTTTTTGCGT
ATTTGATGTGTCTTTATGGAAATCATAAAACTTCAGCCTTCCTTGGATTTTGTGGCTTCATGTTTCGGCA
AACAAATATCATCTGGGCTGTCTTCTGTGCAGGAAATGTCATTGCACAAAAGTTAACGGAGGCTTGGAAA
ACTGAGCTACAAAAGAAGGAAGACAGACTTCCACCTATTAAAGGACCATTTGCAGAATTCAGAAAAATTC
TTCAGTTTCTTTTGGCTTATTCCATGTCCTTTAAAAACTTGAGTATGCTTTTGCTTCTGACTTGGCCCTA
CATCCTTCTGGGATTTCTGTTTTGTGCTTTTGTAGTAGTTAATGGTGGAATTGTTATTGGCGATCGGAGT
AGTCATGAAGCCTGTCTTCATTTTCCTCAACTATTCTACTTTTTTTCATTTACTCTCTTTTTTTCCTTTC
CTCATCTCCTGTCTCCTAGCAAAATTAAGACTTTTCTTTCCTTAGTTTGGAAACGTAGAATTCTGTTTTT
TGTGGTTACCTTAGTCTCTGTGTTTTTAGTTTGGAAATTCACTTATGCTCATAAATACTTGCTAGCAGAC
AATAGACATTATACTTTCTATGTGTGGAAAAGAGTTTTTCAAAGATATGAAACTGTAAAATATTTGTTAG
TTCCAGCCTATATATTTGCTGGTTGGAGTATAGCTGACTCATTGAAATCAAAGTCAATTTTTTGGAATTT
AATGTTTTTCATATGCTTGTTCACTGTTATAGTTCCTCAGAAACTGCTGGAATTTCGTTACTTCATTTTA
CCTTATGTCATTTATAGGCTTAACATACCTCTGCCTCCCACATCCAGACTCATTTGTGAACTGAGCTGCT
ATGCAGTTGTTAATTTCATAACTTTTTTCATCTTTCTGAACAAGACTTTTCAGTGGCCAAATAGTCAGGA
CATTCAAAGGTTTATGTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223859 representing NM_032834
Red=Cloning site Green=Tags(s)

MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGHFSLSQWDPMITTLPGLYL
VSIGVIKPAIWIFGWSEHVVCSIGMLRFVNLLFSVGNFYLLYLLFCKVQPRNKAASSIQRVLSTLTLAVF
PTLYFFNFLYYTEAGSMFFTLFAYLMCLYGNHKTSAFLGFCGFMFRQTNIIWAVFCAGNVIAQKLTEAWK
TELQKKEDRLPPIKGPFAEFRKILQFLLAYSMSFKNLSMLLLLTWPYILLGFLFCAFVVVNGGIVIGDRS
SHEACLHFPQLFYFFSFTLFFSFPHLLSPSKIKTFLSLVWKRRILFFVVTLVSVFLVWKFTYAHKYLLAD
NRHYTFYVWKRVFQRYETVKYLLVPAYIFAGWSIADSLKSKSIFWNLMFFICLFTVIVPQKLLEFRYFIL
PYVIYRLNIPLPPTSRLICELSCYAVVNFITFFIFLNKTFQWPNSQDIQRFMW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032834
ORF Size 1419 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032834.4
RefSeq Size 3143 bp
RefSeq ORF 1422 bp
Locus ID 84920
UniProt ID Q5BKT4
Cytogenetics 12p11.1
Protein Families Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
MW 55.4 kDa
Summary This gene encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel. provided by RefSeq, Jan 2010
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_032834" in other vectors (4)
SKU Description Size Price
RC223859L3 Lenti ORF clone of Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog (S. pombe) (ALG10), Myc-DDK-tagged 10 ug
$757.00
RC223859L4 Lenti ORF clone of Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog (S. pombe) (ALG10), mGFP tagged 10 ug
$757.00
RG223859 ALG10 (tGFP-tagged) - Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog (S. pombe) (ALG10) 10 ug
$657.00
SC317781 ALG10 (untagged)-Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog (S. pombe) (ALG10) 10 ug
$503.00

Plasmid Map for RC223859

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223859
Circular map for RC223859

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products