FBXW7 (NM_018315) Human Tagged ORF Clone

SKU RC223843

FBXW7 (Myc-DDK-tagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FBXW7
• Synonyms: AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223843 representing NM_018315; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTGTGTCCCGAGAAGCGGTTTGATACTGAGCTGCATTTGCCTTTACTGTGGAGTTTTGTTGCCGGTTC; TGCTCCCTAATCTTCCTTTTCTGACGTGCCTGAGCATGTCCACATTAGAATCTGTGACATACCTACCTGA; AAAAGGTTTATATTGTCAGAGACTGCCAAGCAGCCGGACACACGGGGGCACAGAATCACTGAAGGGGAAA; AATACAGAAAATATGGGTTTCTACGGCACATTAAAAATGATTTTTTACAAAATGAAAAGAAAGTTGGACC; ATGGTTCTGAGGTCCGCTCTTTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGAATATACAAGTACCAC; TGGGCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACCTCAGAGCAGCCAATGGCCAAGGGCAA; CAACGACGCCGAATTACATCTGTCCAGCCACCTACAGGCCTCCAGGAATGGCTAAAAATGTTTCAGAGCT; GGAGTGGACCAGAGAAATTGCTTGCTTTAGATGAACTCATTGATAGTTGTGAACCAACACAAGTAAAACA; TATGATGCAAGTGATAGAACCCCAGTTTCAACGAGACTTCATTTCATTGCTCCCTAAAGAGTTGGCACTC; TATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTACAAGCAGCTCAGACATGTCGCTACTGGAGAATTT; TGGCTGAAGACAACCTTCTCTGGAGAGAGAAATGCAAAGAAGAGGGGATTGATGAACCATTGCACATCAA; GAGAAGAAAAGTAATAAAACCAGGTTTCATACACAGTCCATGGAAAAGTGCATACATCAGACAGCACAGA; ATTGATACTAACTGGAGGCGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGACATGATGATCATGTGA; TCACATGCTTACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTC; AGCAGTCACAGGCAAATGTCTGAGAACATTAGTGGGACATACAGGTGGAGTATGGTCATCACAAATGAGA; GACAACATCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAGACTGGAGAATGTA; TACACACCTTATATGGGCATACTTCCACTGTGCGTTGTATGCATCTTCATGAAAAAAGAGTTGTTAGCGG; TTCTCGAGATGCCACTCTTAGGGTTTGGGATATTGAGACAGGCCAGTGTTTACATGTTTTGATGGGTCAT; GTTGCAGCAGTCCGCTGTGTTCAATATGATGGCAGGAGGGTTGTTAGTGGAGCATATGATTTTATGGTAA; AGGTGTGGGATCCAGAGACTGAAACCTGTCTACACACGTTGCAGGGGCATACTAATAGAGTCTATTCATT; ACAGTTTGATGGTATCCATGTGGTGAGTGGATCTCTTGATACATCAATCCGTGTTTGGGATGTGGAGACA; GGGAATTGCATTCACACGTTAACAGGGCACCAGTCGTTAACAAGTGGAATGGAACTCAAAGACAATATTC; TTGTCTCTGGGAATGCAGATTCTACAGTTAAAATCTGGGATATCAAAACAGGACAGTGTTTACAAACATT; GCAAGGTCCCAACAAGCATCAGAGTGCTGTGACCTGTTTACAGTTCAACAAGAACTTTGTAATTACCAGC; TCAGATGATGGAACTGTAAAACTATGGGACTTGAAAACGGGTGAATTTATTCGAAACCTAGTCACATTGG; AGAGTGGGGGGAGTGGGGGAGTTGTGTGGCGGATCAGAGCCTCAAACACAAAGCTGGTGTGTGCAGTTGG; GAGTCGGAATGGGACTGAAGAAACCAAGCTGCTGGTGCTGGACTTTGATGTGGACATGAAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223843 representing NM_018315; Red=Cloning site Green=Tags(s); ; MCVPRSGLILSCICLYCGVLLPVLLPNLPFLTCLSMSTLESVTYLPEKGLYCQRLPSSRTHGGTESLKGK; NTENMGFYGTLKMIFYKMKRKLDHGSEVRSFSLGKKPCKVSEYTSTTGLVPCSATPTTFGDLRAANGQGQ; QRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELAL; YVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKRRKVIKPGFIHSPWKSAYIRQHR; IDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMR; DNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGH; VAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVET; GNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITS; SDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018315
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018315.5
• RefSeq Size: 3603 bp
• RefSeq ORF: 1884 bp
• Locus ID: 55294
• UniProt ID: Q969H0
• Cytogenetics: 4q31.3
• Domains: F-box, WD40
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 70.1 kDa
• Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]