PREX2 (NM_024870) Human Tagged ORF Clone

SKU RC223671

PREX2 (Myc-DDK-tagged)-Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PREX2
• Synonyms: DEP.2; DEPDC2; P-REX2; PPP1R129
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223671 representing NM_024870; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCGAGGACAGCCGCGGAGACAGCCGCGCCGAGAGCGCCAAGGACCTGGAGAAGCAGCTTCGCCTGC; GCGTGTGCGTGCTCAGCGAGCTCCAGAAGACCGAGCGGGACTATGTGGGCACGCTGGAGTTCCTGGTGTC; GGCATTCTTACACAGAATGAACCAGTGTGCAGCATCAAAAGTTGACAAAAATGTGACAGAAGAAACAGTG; AAGATGTTGTTCTCAAACATTGAAGACATCCTTGCAGTACATAAAGAATTCTTAAAAGTCGTGGAAGAAT; GCTTACACCCCGAACCTAATGCTCAACAAGAAGTGGGAACCTGCTTTCTTCACTTTAAAGACAAGTTTCG; TATCTATGATGAATATTGTAGTAACCATGAGAAGGCACAAAAATTACTTCTTGAACTCAACAAAATAAGA; ACAATCCGGACATTTCTTTTGAACTGCATGCTGCTTGGAGGACGGAAGAACACAGATGTTCCCTTGGAAG; GATATTTAGTAACACCAATACAAAGAATATGCAAGTACCCTCTTATTTTGAAGGAGTTGCTGAAGCGGAC; TCCACGGAAACACAGTGACTATGCAGCAGTGATGGAAGCCCTCCAAGCCATGAAAGCTGTCTGTTCCAAC; ATAAACGAGGCCAAGAGACAGATGGAGAAGTTAGAAGTTTTAGAGGAATGGCAGTCTCACATTGAAGGCT; GGGAGGGGTCCAACATCACTGACACCTGCACTGAAATGCTAATGTGTGGAGTCTTACTGAAAATTTCTTC; TGGAAATATTCAAGAACGGGTGTTTTTTCTTTTCGATAATCTTTTGGTGTACTGCAAAAGAAAACACAGA; CGGTTGAAGAACAGCAAGGCATCTACAGATGGACATCGGTACCTTTTTCGTGGCCGGATCAACACGGAGG; TGATGGAAGTGGAGAATGTGGATGATGGCACCGCTGATTTCCATAGCAGTGGACACATTGTTGTTAATGG; ATGGAAGATACATAACACAGCAAAAAATAAATGGTTTGTTTGTATGGCAAAAACACCTGAAGAGAAGCAT; GAATGGTTTGAAGCTATTTTGAAAGAAAGAGAACGGCGGAAAGGTTTAAAATTAGGAATGGAGCAAGATA; CCTGGGTCATGATCTCTGAACAGGGTGAGAAACTTTATAAAATGATGTGCAGACAAGGAAATCTGATCAA; AGACCGAAAGAGAAAACTGACTACGTTCCCTAAATGCTTTCTTGGAAGCGAATTTGTGTCATGGCTGTTG; GAAATTGGAGAGATTCACAGGCCTGAGGAAGGCGTGCACTTGGGACAAGCATTATTAGAAAATGGAATCA; TTCACCATGTTACTGATAAACATCAATTCAAACCAGAACAGATGTTATATAGATTTCGCTATGATGATGG; AACATTTTATCCAAGAAATGAGATGCAGGACGTGATTTCAAAGGGTGTAAGATTATATTGTCGTCTTCAT; AGCCTTTTTACTCCAGTGATAAGAGATAAAGATTACCATTTAAGGACCTACAAATCTGTGGTCATGGCCA; ACAAACTGATAGACTGGTTAATTGCACAGGGAGATTGCCGCACCAGAGAAGAGGCAATGATATTTGGCGT; TGGACTCTGTGACAATGGATTTATGCACCATGTCCTTGAAAAAAGCGAATTCAAAGATGAACCCCTACTT; TTCCGTTTTTTTTCGGATGAGGAAATGGAGGGATCAAATATGAAACATCGACTTATGAAACATGACTTAA; AAGTTGTGGAAAATGTTATAGCTAAGTCATTATTGATTAAATCCAATGAAGGCAGCTATGGCTTTGGATT; AGAAGACAAAAATAAAGTTCCAATAATAAAGTTGGTAGAAAAGGGATCTAATGCTGAGATGGCTGGCATG; GAAGTCGGGAAAAAGATTTTTGCTATTAATGGTGACCTAGTTTTTATGAGACCTTTCAATGAAGTGGATT; GCTTCCTGAAATCGTGTTTAAACAGCAGAAAACCTCTAAGAGTTCTTGTGAGCACAAAGCCAAGAGAGAC; AGTGAAAATTCCAGATTCAGCTGATGGACTTGGCTTCCAGATCCGGGGATTTGGCCCTTCTGTTGTGCAT; GCTGTAGGAAGAGGAACTGTGGCTGCAGCAGCTGGTCTTCACCCTGGACAGTGCATTATCAAGGTGAATG; GAATCAATGTCAGCAAAGAGACACATGCCAGTGTCATTGCACACGTTACAGCCTGCAGGAAGTACAGGCG; GCCAACGAAGCAAGATTCCATACAATGGGTTTATAATAGCATTGAGAGTGCTCAAGAAGACCTTCAAAAA; TCTCACTCCAAGCCCCCTGGAGATGAAGCAGGGGATGCTTTTGACTGTAAAGTAGAAGAGGTTATTGACA; AATTCAACACTATGGCCATCATTGATGGGAAGAAGGAGCATGTGAGTCTGACAGTGGACAATGTCCACCT; GGAATATGGTGTCGTGTATGAGTACGACAGCACAGCTGGCATCAAGTGCAATGTGGTGGAAAAGATGATT; GAGCCCAAAGGTTTCTTCAGCTTAACTGCCAAGATTCTTGAAGCCCTGGCTAAAAGTGATGAGCATTTTG; TACAAAACTGTACCAGCCTAAATTCTCTAAATGAAGTGATTCCTACTGACCTTCAGAGTAAATTCAGTGC; CCTTTGCAGTGAAAGAATTGAACACCTATGTCAGAGAATATCCAGTTATAAAAAGTTTTCTCGTGTACTG; AAGAATAGGGCCTGGCCTACTTTTAAACAGGCCAAATCTAAAATCTCCCCACTGCACAGCAGTGATTTCT; GCCCTACCAACTGCCATGTCAATGTGATGGAAGTTTCTTATCCCAAAACATCAACCTCTTTGGGAAGTGC; ATTTGGTGTTCAGTTGGATAGCAGGAAGCATAATTCTCATGATAAAGAAAACAAATCTTCGGAGCAAGGG; AAACTGAGCCCTATGGTGTACATTCAGCACACCATCACAACCATGGCGGCCCCTTCAGGTCTGTCTCTGG; GACAGCAGGATGGCCATGGTCTCAGGTATCTGCTAAAAGAAGAAGACTTAGAAACCCAAGACATCTATCA; GAAACTGCTGGGCAAACTTCAGACTGCACTGAAAGAGGTGGAGATGTGTGTTTGTCAAATAGATGACCTT; CTGTCTTCAATAACATATTCTCCTAAATTAGAACGTAAGACATCAGAGGGCATAATACCAACAGACAGTG; ACAATGAGAAGGGAGAAAGAAACAGCAAACGGGTATGTTTTAATGTAGCAGGAGATGAACAGGAAGATTC; TGGTCATGACACCATCAGCAACAGAGACTCTTACAGTGACTGCAACAGCAATAGGAATTCCATCGCCTCC; TTCACCAGCATCTGCAGCAGCCAGTGCAGCTCGTATTTCCACAGTGATGAAATGGACTCAGGTGATGAAC; TTCCCTTAAGTGTTCGCATATCTCATGATAAACAGGACAAGATACATAGTTGCCTTGAGCATCTTTTCAG; CCAGGTGGATTCAATTACCAATCTCCTAAAAGGGCAGGCTGTTGTGAGGGCCTTTGACCAAACCAAGTAT; CTCACTCCAGGCCGAGGATTGCAAGAATTTCAACAGGAAATGGAACCAAAGCTGAGTTGTCCAAAAAGGC; TACGGCTTCATATCAAGCAAGATCCTTGGAATCTTCCCAGCAGCGTCCGGACTCTTGCTCAGAACATCAG; GAAATTTGTTGAAGAGGTAAAGTGTAGGCTACTCCTGGCTCTTCTTGAATATTCAGATAGTGAGACACAG; CTCCGTAGAGACATGGTTTTCTGCCAGACTCTTGTGGCCACTGTCTGTGCCTTCTCTGAGCAGCTCATGG; CGGCCTTGAACCAGATGTTTGACAACAGCAAGGAAAATGAGATGGAAACTTGGGAAGCCAGCAGGAGGTG; GCTGGACCAGATAGCGAATGCAGGTGTTCTTTTTCACTTTCAGTCACTTCTGTCACCAAACTTGACAGAT; GAACAAGCCATGTTAGAAGATACACTGGTTGCACTATTTGATTTGGAAAAGGTTTCCTTTTACTTTAAAC; CATCAGAAGAGGAACCTCTGGTTGCAAATGTTCCTCTTACATATCAAGCAGAAGGAAGTCGGCAAGCTCT; GAAAGTTTACTTCTACATTGATAGTTATCATTTTGAACAACTTCCTCAACGGCTGAAAAATGGAGGAGGG; TTTAAAATTCATCCTGTTCTTTTTGCACAAGCACTAGAGAGCATGGAAGGATATTATTACAGAGACAATG; TTTCTGTGGAAGAATTTCAAGCTCAGATAAATGCAGCCTCACTGGAAAAGGTCAAACAGTACAACCAGAA; GCTCAGGGCATTCTACTTGGACAAGTCAAATTCACCACCAAACTCCACATCCAAAGCTGCCTATGTAGAT; AAGCTAATGAGGCCTCTCAACGCTTTGGATGAACTTTACCGACTGGTAGCCTCGTTTATCAGATCCAAGC; GCACAGCTGCCTGTGCAAACACAGCTTGCAGTGCTTCTGGGGTTGGACTGCTGTCAGTTTCCTCGGAGCT; GTGCAACAGGCTGGGCGCCTGCCACATCATCATGTGCAGCAGCGGTGTGCATCGGTGCACCCTGAGCGTG; ACGCTGGAGCAAGCCATCATTCTGGCCAGAAGCCACGGACTGCCACCTCGTTACATCATGCAGGCTACAG; ATGTGATGCGGAAGCAGGGAGCAAGAGTTCAGAACACAGCGAAGAATTTGGGAGTCAGAGACCGGACTCC; ACAGTCTGCACCAAGGCTGTACAAGCTGTGCGAGCCACCTCCCCCAGCTGGAGAAGAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223671 representing NM_024870; Red=Cloning site Green=Tags(s); ; MSEDSRGDSRAESAKDLEKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHRMNQCAASKVDKNVTEETV; KMLFSNIEDILAVHKEFLKVVEECLHPEPNAQQEVGTCFLHFKDKFRIYDEYCSNHEKAQKLLLELNKIR; TIRTFLLNCMLLGGRKNTDVPLEGYLVTPIQRICKYPLILKELLKRTPRKHSDYAAVMEALQAMKAVCSN; INEAKRQMEKLEVLEEWQSHIEGWEGSNITDTCTEMLMCGVLLKISSGNIQERVFFLFDNLLVYCKRKHR; RLKNSKASTDGHRYLFRGRINTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCMAKTPEEKH; EWFEAILKERERRKGLKLGMEQDTWVMISEQGEKLYKMMCRQGNLIKDRKRKLTTFPKCFLGSEFVSWLL; EIGEIHRPEEGVHLGQALLENGIIHHVTDKHQFKPEQMLYRFRYDDGTFYPRNEMQDVISKGVRLYCRLH; SLFTPVIRDKDYHLRTYKSVVMANKLIDWLIAQGDCRTREEAMIFGVGLCDNGFMHHVLEKSEFKDEPLL; FRFFSDEEMEGSNMKHRLMKHDLKVVENVIAKSLLIKSNEGSYGFGLEDKNKVPIIKLVEKGSNAEMAGM; EVGKKIFAINGDLVFMRPFNEVDCFLKSCLNSRKPLRVLVSTKPRETVKIPDSADGLGFQIRGFGPSVVH; AVGRGTVAAAAGLHPGQCIIKVNGINVSKETHASVIAHVTACRKYRRPTKQDSIQWVYNSIESAQEDLQK; SHSKPPGDEAGDAFDCKVEEVIDKFNTMAIIDGKKEHVSLTVDNVHLEYGVVYEYDSTAGIKCNVVEKMI; EPKGFFSLTAKILEALAKSDEHFVQNCTSLNSLNEVIPTDLQSKFSALCSERIEHLCQRISSYKKFSRVL; KNRAWPTFKQAKSKISPLHSSDFCPTNCHVNVMEVSYPKTSTSLGSAFGVQLDSRKHNSHDKENKSSEQG; KLSPMVYIQHTITTMAAPSGLSLGQQDGHGLRYLLKEEDLETQDIYQKLLGKLQTALKEVEMCVCQIDDL; LSSITYSPKLERKTSEGIIPTDSDNEKGERNSKRVCFNVAGDEQEDSGHDTISNRDSYSDCNSNRNSIAS; FTSICSSQCSSYFHSDEMDSGDELPLSVRISHDKQDKIHSCLEHLFSQVDSITNLLKGQAVVRAFDQTKY; LTPGRGLQEFQQEMEPKLSCPKRLRLHIKQDPWNLPSSVRTLAQNIRKFVEEVKCRLLLALLEYSDSETQ; LRRDMVFCQTLVATVCAFSEQLMAALNQMFDNSKENEMETWEASRRWLDQIANAGVLFHFQSLLSPNLTD; EQAMLEDTLVALFDLEKVSFYFKPSEEEPLVANVPLTYQAEGSRQALKVYFYIDSYHFEQLPQRLKNGGG; FKIHPVLFAQALESMEGYYYRDNVSVEEFQAQINAASLEKVKQYNQKLRAFYLDKSNSPPNSTSKAAYVD; KLMRPLNALDELYRLVASFIRSKRTAACANTACSASGVGLLSVSSELCNRLGACHIIMCSSGVHRCTLSV; TLEQAIILARSHGLPPRYIMQATDVMRKQGARVQNTAKNLGVRDRTPQSAPRLYKLCEPPPPAGEE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024870
• ORF Size: 4818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024870.4
• RefSeq Size: 5132 bp
• RefSeq ORF: 4821 bp
• Locus ID: 80243
• UniProt ID: Q70Z35
• Cytogenetics: 8q13.2
• MW: 182.4 kDa
• Summary: The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers. [provided by RefSeq, Apr 2016]