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RASGRF2 (NM_006909) Human Tagged ORF Clone

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SKU
RC223580
RASGRF2 (Myc-DDK-tagged)-Human Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,077.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RASGRF2
Synonyms GRF2; RAS-GRF2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223580 representing NM_006909
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAAGAGCGTGCGCTACAACGAGGGGCACGCCCTGTACCTGGCCTTTCTGGCGCGCAAGGAGGGCA
CCAAGCGCGGCTTCCTGAGTAAGAAGACGGCCGAGGCGAGCCGCTGGCACGAGAAGTGGTTCGCCCTCTA
CCAGAATGTGCTCTTCTACTTCGAGGGCGAGCAGAGCTGCCGCCCGGCGGGCATGTACCTCCTGGAGGGC
TGCAGCTGCGAACGAACGCCCGCGCCACCCAGGGCCGGCGCCGGGCAGGGAGGCGTCCGAGACGCGCTGG
ACAAGCAGTATTACTTTACTGTTCTTTTTGGCCATGAAGGTCAGAAGCCACTGGAGCTGCGCTGTGAGGA
GGAGCAGGATGGTAAAGAGTGGATGGAGGCCATTCACCAAGCCAGTTATGCAGACATTTTGATTGAGAGG
GAAGTATTAATGCAGAAGTACATTCATCTAGTTCAGATCGTAGAGACAGAAAAAATTGCAGCTAACCAAC
TCCGACATCAACTTGAAGATCAAGACACAGAAATCGAAAGGCTTAAATCAGAGATTATTGCTCTTAATAA
AACCAAAGAACGAATGCGACCTTACCAAAGCAACCAAGAAGACGAAGATCCAGACATCAAGAAGATTAAA
AAGGTTCAGAGCTTCATGCGAGGATGGTTGTGCAGAAGGAAATGGAAGACCATCGTGCAGGATTACATTT
GTTCTCCTCATGCTGAAAGTATGAGGAAGAGAAACCAGATTGTGTTCACCATGGTGGAGGCAGAGTCAGA
GTACGTTCACCAGCTCTACATCCTGGTCAATGGCTTTCTCCGGCCCCTGCGTATGGCCGCCAGCTCCAAG
AAGCCCCCCATCAGCCACGACGACGTCAGCAGTATTTTTCTTAACAGTGAAACAATCATGTTTCTTCATG
AAATATTTCATCAAGGACTAAAGGCAAGGATAGCAAACTGGCCCACTTTAATTTTAGCTGATCTGTTTGA
TATTTTGCTCCCCATGCTGAACATTTATCAAGAATTTGTGCGTAATCACCAGTACAGCCTGCAAGTTCTC
GCCAATTGTAAGCAAAACAGAGATTTTGACAAACTCTTAAAACAGTATGAAGCCAATCCAGCCTGTGAGG
GGAGGATGCTGGAGACATTCTTGACCTATCCCATGTTTCAGATCCCCAGATATATCATCACACTCCATGA
GCTCCTTGCTCACACACCCCATGAGCATGTGGAAAGGAAAAGCCTGGAGTTTGCCAAATCAAAGCTAGAG
GAACTATCCAGAGTAATGCACGATGAAGTCAGCGACACTGAAAACATAAGGAAAAACCTTGCCATCGAAA
GAATGATCGTGGAGGGCTGTGACATCTTGCTGGACACCAGCCAAACGTTCATCCGCCAAGGTTCTCTTAT
TCAAGTACCTTCCGTTGAGAGGGGGAAACTTAGTAAAGTTCGCCTGGGTTCGTTGTCTTTGAAAAAGGAA
GGAGAGAGACAATGCTTCTTATTTACAAAACACTTTTTAATATGTACAAGAAGTTCAGGAGGGAAGCTTC
ATCTGCTCAAGACAGGTGGGGTTCTGTCTCTAATAGACTGCACATTGATTGAGGAGCCAGATGCAAGCGA
TGATGACTCTAAAGGTTCTGGGCAAGTGTTTGGGCACCTGGATTTTAAAATAGTGGTGGAGCCTCCTGAC
GCTGCCGCCTTCACTGTTGTCTTGTTAGCACCCTCACGCCAGGAGAAAGCTGCCTGGATGAGTGACATCA
GTCAGTGTGTGGACAATATACGATGTAATGGTTTAATGACTATAGTGTTTGAAGAGAATTCCAAAGTCAC
TGTGCCACATATGATTAAGTCTGATGCCCGTCTTCATAAAGACGACACTGACATTTGCTTCAGTAAAACA
CTCAACTCCTGCAAAGTGCCCCAGATCCGTTATGCCAGCGTGGAGCGCCTCTTGGAACGACTGACAGACT
TGCGGTTTCTTAGTATTGATTTCCTCAACACCTTTCTGCACACCTATCGTATTTTCACTACTGCCGCTGT
GGTGCTGGGGAAACTCTCCGACATATACAAGAGGCCTTTCACCTCCATCCCTGTCAGGTCATTGGAATTG
TTTTTTGCTACCAGCCAGAACAACAGAGGTGAACATTTGGTGGATGGCAAATCCCCACGTCTGTGTCGCA
AATTCTCTTCCCCGCCACCACTGGCTGTGTCCAGAACATCTTCCCCAGTGAGGGCCAGAAAGCTGTCTTT
GACTTCTCCCTTGAACTCAAAGATAGGAGCATTGGACCTGACAACTTCCAGCAGTCCCACCACCACCACC
CAGAGTCCCGCTGCGTCTCCACCACCACACACTGGTCAGATACCACTGGATCTCAGCAGAGGCCTCTCTT
CTCCAGAGCAAAGCCCGGGAACGGTAGAAGAGAATGTCGATAACCCACGCGTGGATCTGTGTAACAAGCT
AAAACGAAGTATTCAAAAAGCAGTCCTAGAGTCTGCACCAGCGGACCGAGCAGGAGTGGAAAGCTCCCCT
GCAGCGGACACCACAGAACTTTCACCTTGCAGATCCCCCTCAACTCCTCGGCACCTCCGCTATCGACAGC
CTGGAGGACAGACGGCGGACAATGCCCACTGCTCTGTTTCACCGGCTTCTGCTTTTGCAATAGCCACAGC
TGCAGCAGGACATGGGAGTCCACCAGGCTTTAACAACACCGAGAGAACATGTGATAAAGAGTTTATTATA
CGGAGAACGGCTACCAATCGAGTTCTGAACGTCCTCCGTCACTGGGTCTCAAAGCACGCACAGGATTTCG
AACTCAACAATGAACTAAAGATGAATGTCCTAAATTTGCTAGAAGAAGTTTTGCGAGACCCAGACCTTCT
TCCCCAAGAAAGGAAAGCCGCCGCGAATATCCTCAGGGCCCTTTCACAAGATGACCAAGATGACATCCAC
CTAAAATTAGAGGATATAATTCAAATGACTGACTGCATGAAGGCCGAATGCTTTGAGTCCTTGTCGGCCA
TGGAGCTGGCAGAACAGATCACCCTCCTGGACCATGTCATTTTCAGAAGCATTCCCTACGAGGAGTTTCT
TGGGCAGGGGTGGATGAAGCTGGATAAAAACGAAAGAACTCCTTACATTATGAAAACCAGCCAACACTTC
AATGACATGAGTAACCTGGTGGCCTCCCAGATAATGAACTATGCTGATGTCAGCTCCCGTGCCAACGCCA
TCGAGAAATGGGTGGCAGTGGCGGACATCTGCCGATGCCTGCACAACTACAACGGCGTGCTGGAGATCAC
CTCGGCCTTAAACAGAAGTGCCATCTACAGGCTGAAGAAAACCTGGGCCAAGGTCTCTAAGCAGACAAAA
GCTCTAATGGACAAACTTCAAAAGACTGTTTCCTCTGAAGGAAGATTTAAAAATCTTAGAGAAACCCTTA
AAAATTGTAACCCTCCTGCAGTTCCTTATCTTGGGATGTACTTGACAGACCTGGCATTTATTGAAGAAGG
AACACCAAACTTTACTGAGGAAGGCCTTGTCAATTTCTCCAAAATGAGAATGATATCACACATCATCAGA
GAGATACGCCAGTTCCAGCAGACTTCCTACAGAATAGATCATCAGCCAAAGGTCGCACAGTACTTGCTTG
ACAAAGACCTTATCATAGATGAAGATACGCTATATGAGCTGTCACTAAAAATTGAACCTCGACTCCCTGC
T

ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
TTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223580 representing NM_006909
Red=Cloning site Green=Tags(s)

MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKTAEASRWHEKWFALYQNVLFYFEGEQSCRPAGMYLLEG
CSCERTPAPPRAGAGQGGVRDALDKQYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIER
EVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIK
KVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSK
KPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVL
ANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLE
ELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKE
GERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIDCTLIEEPDASDDDSKGSGQVFGHLDFKIVVEPPD
AAAFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKT
LNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLEL
FFATSQNNRGEHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSKIGALDLTTSSSPTTTT
QSPAASPPPHTGQIPLDLSRGLSSPEQSPGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSP
AADTTELSPCRSPSTPRHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTERTCDKEFII
RRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKAAANILRALSQDDQDDIH
LKLEDIIQMTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHF
NDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTK
ALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIR
EIRQFQQTSYRIDHQPKVAQYLLDKDLIIDEDTLYELSLKIEPRLPA

TRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-NotI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006909
ORF Size 3711 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006909.3
RefSeq Size 4078 bp
RefSeq ORF 3714 bp
Locus ID 5924
UniProt ID O14827, A0A2X0SFL3, Q68DX5
Cytogenetics 5q14.1
Protein Pathways MAPK signaling pathway
MW 140.6 kDa
Summary RAS GTPases cycle between an inactive GDP-bound state and an active GTP-bound state. This gene encodes a calcium-regulated nucleotide exchange factor activating both RAS and RAS-related protein, RAC1, through the exchange of bound GDP for GTP, thereby, coordinating the signaling of distinct mitogen-activated protein kinase pathways. provided by RefSeq, Oct 2011
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"NM_006909" in other vectors (6)
SKU Description Size Price
RC223580L1 Lenti ORF clone of Human Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), Myc-DDK-tagged 10 ug
$1,377.00
RC223580L2 Lenti ORF clone of Human Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), mGFP tagged 10 ug
$1,377.00
RC223580L3 Lenti ORF clone of Human Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), Myc-DDK-tagged 10 ug
$1,377.00
RC223580L4 Lenti ORF clone of Human Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), mGFP tagged 10 ug
$1,377.00
RG223580 RASGRF2 (tGFP-tagged) - Human Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2) 10 ug
$1,277.00
SC315292 RASGRF2 (untagged)-Human Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2) 10 ug
$1,078.00

Plasmid Map for RC223580

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223580
Circular map for RC223580

file-alt Citations

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