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DDX31 (NM_022779) Human Tagged ORF Clone

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SKU
RC223495
DDX31 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $782.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DDX31
Synonyms PPP1R25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223495 representing NM_022779
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCGGATCTCGCTTCTCAGAGGCATTCAGAGAGCTTCCCCAGCGTGAACTCTCGGCCGAATGTAA
TTTTGCCAGGCCGCGAGGGACGCAGAGAGGGGCTGCCACCAGGCGGGGGCACGCGCGGTAGTCTTGTCCC
TACCCGCCCGGTGCCGCCGTCCCCGGCTCCCCTCGGGACGAGTCCGTACAGCTGGAGCCGTTCCGGACCC
GGAAGGGGCGGTGGCGCAGGGTCCTCGCGCGTCCCACGTGGGGTCCCGGGCCCAGCAGTCTGCGCACCGG
GCTCGCTGCTGCACCACGCGTCACCCACGCAGACCATGGCAGCCGCCGACGGTTCGCTCTTCGACAACCC
CAGGACGTTCTCCAGACGTCCCCCAGCCCAGGCGAGTCGGCAAGCAAAGGCTACGAAAAGAAAATACCAA
GCGTCCAGTGAGGCTCCCCCAGCGAAACGGAGGAACGAAACTTCATTTCTCCCAGCCAAGAAAACTAGTG
TTAAAGAAACTCAGAGGACTTTTAAGGGGAACGCACAAAAAATGTTTTCTCCAAAGAAGCATTCGGTTAG
CACAAGTGATAGAAACCAGGAGGAGAGACAGTGCATTAAGACTTCATCACTGTTTAAAAACAACCCTGAC
ATTCCAGAACTCCACAGACCTGTGGTAAAGCAGGTGCAAGAAAAAGTGTTTACTTCAGCTGCTTTTCATG
AGCTGGGCCTCCACCCACATTTAATTTCCACAATAAATACGGTCTTAAAAATGTCTAGTATGACCAGTGT
TCAGAAGCAAAGTATTCCTGTGTTGCTGGAAGGCAGAGATGCTCTCGTGAGATCCCAGACGGGCTCAGGT
AAAACTCTTGCCTATTGCATCCCTGTGGTCCAGTCCCTTCAAGCAATGGAGTCAAAAATACAGCGCAGTG
ATGGCCCCTATGCCCTGGTGCTCGTGCCAACGAGAGAGCTAGCTCTACAAAGCTTTGACACTGTCCAGAA
ACTGCTTAAGCCTTTCACCTGGATTGTGCCTGGAGTGTTAATGGGAGGAGAGAAGAGAAAATCAGAAAAG
GCCAGACTCCGCAAAGGAATAAATATCCTTATCTCAACTCCTGGACGCCTGGTGGATCATATAAAATCCA
CAAAGAACATTCATTTTAGTCGGCTGCGGTGGTTGGTGTTTGATGAAGCAGACAGAATCTTGGATTTGGG
TTTTGAAAAGGACATCACAGTGATACTTAATGCTGTAAATGCTGAATGCCAAAAACGACAGAATGTCTTG
CTATCAGCGACACTCACAGAAGGTGTAACGCGGCTAGCTGATATCAGTTTGCATGATCCAGTCAGTATTT
CTGTCCTGGACAAGAGCCATGACCAGTTGAACCCAAAGGACAAAGCGGTCCAGGAGGTCTGTCCTCCACC
AGCTGGCGACAAGCTGGACAGCTTTGCAATACCAGAGAGTCTCAAGCAGCATGTGACTGTGGTTCCCAGC
AAACTGAGGCTTGTCTGCCTAGCGGCCTTCATCCTTCAGAAATGCAAGTTTGAGGAAGACCAGAAGATGG
TTGTCTTTTTCTCAAGTTGCGAGCTGGTGGAGTTCCACTACAGCCTCTTCCTACAGACCCTGCTGAGCAG
CTCAGGGGCGCCGGCATCAGGGCAGTTGCCATCTGCCTCCATGCGATTAAAATTCCTACGGCTGCATGGC
GGCATGGAGCAGGAGGAAAGAACAGCAGTGTTTCAGGAATTTTCACATTCCAGAAGAGGCGTCCTTCTTT
GCACGGATGTTGCAGCTCGGGGCTTAGATCTCCCTCAAGTCACGTGGATTGTTCAGTACAACGCTCCATC
TTCACCTGCAGAATACATCCACCGGATTGGAAGAACCGCCCGGATTGGCTGCCATGGGAGCAGCCTGCTC
ATTTTGGCTCCTTCGGAGGCAGAATATGTCAACTCGTTGGCTTCTCACAAAATCAACGTTTCTGAGATTA
AGATGGAAGATATTTTGTGTGTTCTGACAAGAGATGATTGTTTTAAAGGGAAACGATGGGGAGCCCAGAA
ATCCCATGCTGTTGGCCCCCAGGAAATCCGAGAGCGAGCCACAGTCTTGCAGACGGTATTTGAAGATTAC
GTGCACTCCAGTGAGAGGAGGGTCTCCTGGGCAAAGAAAGCTCTGCAGTCCTTCATCCAAGCCTACGCCA
CCTACCCCAGGGAGCTGAAGCACATCTTCCACGTCCGATCCCTCCACCTTGGGCATGTGGCGAAGAGCTT
CGGACTAAGAGATGCCCCCAGGAATCTTAGTGCCTTGACTAGAAAGAAGAGGAAAGCACACGTGAAAAGG
CCTGACCTTCATAAGAAGACCCAGAGTAAACACAGCCTCGCTGAAATCCTACGTTCGGAATACTCAAGCG
GCATGGAGGCCGACGTCGCCAAGGTCAAAAAGCAAAACGCACCTGGAGAGCCTGGTGGCCGGCCCCTGCA
GCACAGTCTGCAGCCGACACCCTGCTTTGGCCGTGGGAAAACATTAAAATGGAGAAAAACCCAAAAAGGT
GTACAGCGGGACAGCAAGACTTCCCAGAAAGTTAGCGGACCG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223495 representing NM_022779
Red=Cloning site Green=Tags(s)

MAPDLASQRHSESFPSVNSRPNVILPGREGRREGLPPGGGTRGSLVPTRPVPPSPAPLGTSPYSWSRSGP
GRGGGAGSSRVPRGVPGPAVCAPGSLLHHASPTQTMAAADGSLFDNPRTFSRRPPAQASRQAKATKRKYQ
ASSEAPPAKRRNETSFLPAKKTSVKETQRTFKGNAQKMFSPKKHSVSTSDRNQEERQCIKTSSLFKNNPD
IPELHRPVVKQVQEKVFTSAAFHELGLHPHLISTINTVLKMSSMTSVQKQSIPVLLEGRDALVRSQTGSG
KTLAYCIPVVQSLQAMESKIQRSDGPYALVLVPTRELALQSFDTVQKLLKPFTWIVPGVLMGGEKRKSEK
ARLRKGINILISTPGRLVDHIKSTKNIHFSRLRWLVFDEADRILDLGFEKDITVILNAVNAECQKRQNVL
LSATLTEGVTRLADISLHDPVSISVLDKSHDQLNPKDKAVQEVCPPPAGDKLDSFAIPESLKQHVTVVPS
KLRLVCLAAFILQKCKFEEDQKMVVFFSSCELVEFHYSLFLQTLLSSSGAPASGQLPSASMRLKFLRLHG
GMEQEERTAVFQEFSHSRRGVLLCTDVAARGLDLPQVTWIVQYNAPSSPAEYIHRIGRTARIGCHGSSLL
ILAPSEAEYVNSLASHKINVSEIKMEDILCVLTRDDCFKGKRWGAQKSHAVGPQEIRERATVLQTVFEDY
VHSSERRVSWAKKALQSFIQAYATYPRELKHIFHVRSLHLGHVAKSFGLRDAPRNLSALTRKKRKAHVKR
PDLHKKTQSKHSLAEILRSEYSSGMEADVAKVKKQNAPGEPGGRPLQHSLQPTPCFGRGKTLKWRKTQKG
VQRDSKTSQKVSGP

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_022779
ORF Size 2562 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022779.8
RefSeq Size 3285 bp
RefSeq ORF 2241 bp
Locus ID 64794
UniProt ID Q9H8H2
Cytogenetics 9q34.13
MW 93.9 kDa
Summary DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. provided by RefSeq, Apr 2016
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"NM_022779" in other vectors (6)
SKU Description Size Price
RC223495L1 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, Myc-DDK-tagged 10 ug
$1,082.00
RC223495L2 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, mGFP tagged 10 ug
$1,082.00
RC223495L3 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, Myc-DDK-tagged 10 ug
$1,082.00
RC223495L4 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, mGFP tagged 10 ug
$1,082.00
RG223495 DDX31 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1 10 ug
$789.00 $982.00
SC314815 DDX31 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1 10 ug
$780.00

Plasmid Map for RC223495

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223495
Circular map for RC223495

file-alt Citations

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