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DDX31 (NM_022779) Human Untagged Clone

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SKU
SC314815
DDX31 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1
  $780.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DDX31
Synonyms PPP1R25
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_022779 edited
ATTGAGCTCCAGAGCCTGTTAGGGAGCTACCCAGACAGGCTTTAGTTCCTAAAGTGCTGA
GGAAACGCACGGGGCGGTAAAGGGGAGGCTCGTCGGTGAGATGGCGCCGGATCTCGCTTC
TCAGAGGCATTCAGAGAGCTTCCCCAGCGTGAACTCTCGGCCGAATGTAATTTTGCCAGG
CCGCGAGGGACGCAGAGAGGGGCTGCCACCAGGCGGGGGCACGCGCGGTAGTCTTGTCCC
TACCCGCCCGGTGCCGCCGTCCCCGGCTCCCCTCGGGACGAGTCCGTACAGCTGGAGCCG
TTCCGGACCCGGAAGGGGCGGTGGCGCAGGGTCCTCGCGCGTCCCACGTGGGGTCCCGGG
CCCAGCAGTCTGCGCACCGGGCTCGCTGCTGCACCACGCGTCACCCACGCAGACCATGGC
AGCCGCCGACGGTTCGCTCTTCGACAACCCCAGGACGTTCTCCAGACGTCCCCCAGCCCA
GGCGAGTCGGCAAGCAAAGGCTACGAAAAGAAAATACCAAGCGTCCAGTGAGGCTCCCCC
AGCGAAACGGAGGAACGAAACTTCATTTCTCCCAGCCAAGAAAACTAGTGTTAAAGAAAC
TCAGAGGACTTTTAAGGGGAACGCACAAAAAATGTTTTCTCCAAAGAAGCATTCGGTTAG
CACAAGTGATAGAAACCAGGAGGAGAGACAGTGCATTAAGACTTCATCACTGTTTAAAAA
CAACCCTGACATTCCAGAACTCCACAGACCTGTGGTAAAGCAGGTGCAAGAAAAAGTGTT
TACTTCAGCTGCTTTTCATGAGCTGGGCCTCCACCCACATTTAATTTCCACAATAAATAC
GGTCTTAAAAATGTCTAGTATGACCAGTGTTCAGAAGCAAAGTATTCCTGTGTTGCTGGA
AGGCAGAGATGCTCTCGTGAGATCCCAGACGGGCTCAGGTAAAACTCTTGCCTATTGCAT
CCCTGTGGTCCAGTCCCTTCAAGCAATGGAGTCAAAAATACAGCGCAGTGATGGCCCCTA
TGCCCTGGTGCTCGTGCCAACGAGAGAGCTAGCTCTACAAAGCTTTGACACTGTCCAGAA
ACTGCTTAAGCCTTTCACCTGGATTGTGCCTGGAGTGTTAATGGGAGGAGAGAAGAGAAA
ATCAGAAAAGGCCAGACTCCGCAAAGGAATAAATATCCTTATCTCAACTCCTGGACGCCT
GGTGGATCATATAAAATCCACAAAGAACATTCATTTTAGTCGGCTGCGGTGGTTGGTGTT
TGATGAAGCAGACAGAATCTTGGATTTGGGTTTTGAAAAGGACATCACAGTGATACTTAA
TGCTGTAAATGCTGAATGCCAAAAACGACAGAATGTCTTGCTATCAGCGACACTCACAGA
AGGTGTAACGCGGCTAGCTGATATCAGTTTGCATGATCCAGTCAGTATTTCTGTCCTGGA
CAAGAGCCATGACCAGTTGAACCCAAAGGACAAAGCGGTCCAGGAGGTCTGTCCTCCACC
AGCTGGCGACAAGCTGGACAGCTTTGCAATACCAGAGAGTCTCAAGCAGCATGTGACTGT
GGTTCCCAGCAAACTGAGGCTTGTCTGCCTAGCGGCCTTCATCCTTCAGAAATGCAAGTT
TGAGGAAGACCAGAAGATGGTTGTCTTTTTCTCAAGTTGCGAGCTGGTGGAGTTCCACTA
CAGCCTCTTCCTACAGACCCTGCTGAGCAGCTCAGGGGCGCCGGCATCAGGGCAGTTGCC
ATCTGCCTCCATGCGATTAAAATTCCTACGGCTGCATGGCGGCATGGAGCAGGAGGAAAG
AACAGCAGTGTTTCAGGAATTTTCACATTCCAGAAGAGGCGTCCTTCTTTGCACGGATGT
TGCAGCTCGGGGCTTAGATCTCCCTCAAGTCACGTGGATTGTTCAGTACAACGCTCCATC
TTCACCTGCAGAATACATCCACCGGATTGGAAGAACCGCCCGGATTGGCTGCCATGGGAG
CAGCCTGCTCATTTTGGCTCCTTCGGAGGCAGAATATGTCAACTCGTTGGCTTCTCACAA
AATCAACGTTTCTGAGATTAAGATGGAAGATATTTTGTGTGTTCTGACAAGAGATGATTG
TTTTAAAGGGAAACGATGGGGAGCCCAGAAATCCCATGCTGTTGGCCCCCAGGAAATCCG
AGAGCGAGCCACAGTCTTGCAGACGGTATTTGAAGATTACGTGCACTCCAGTGAGAGGAG
GGTCTCCTGGGCAAAGAAAGCTCTGCAGTCCTTCATCCAAGCCTACGCCACCTACCCCAG
GGAGCTGAAGCACATCTTCCACGTCCGATCCCTCCACCTTGGGCATGTGGCGAAGAGCTT
CGGACTAAGAGATGCCCCCAGGAATCTTAGTGCCTTGACTAGAAAGAAGAGGAAAGCACA
CGTGAAAAGGCCTGACCTTCATAAGAAGACCCAGAGTAAACACAGCCTCGCTGAAATCCT
ACGTTCGGAATACTCAAGCGGCATGGAGGCCGACGTCGCCAAGGTCAAAAAGCAAAACGC
ACCTGGAGAGCCTGGTGGCCGGCCCCTGCAGCACAGTCTGCAGCCGACACCCTGCTTTGG
CCGTGGGAAAACATTAAAATGGAGAAAAACCCAAAAAGGTGTACAGCGGGACAGCAAGAC
TTCCCAGAAAGTTTAAAATCTCTCTGGGTCTTCGAGTGGAACCTGGAAGCCCCGGGTGGC
AGTGGATTGATGTCCAGTTCTTGTCAGAGGAGCCTTGTGAAAAATAACTGCGCCTTTGCC
TGAATACCACGTCCCCAAGAATCAGAGGGGCCACAGCCCTTCTCCTCCATGGGAGGCCCT
GGAGCCTGATAGAAATCAGTGGTGTGGGGCACTGTAGAGACTAGCCAGCTCCCGACGGAA
GGGGTCTGTGTCTTGTATTGATCCCATCAGTGCCCGGCACTCCCTTCACCCAGGTGCTGA
CGTGCCGGGGTTGAGCTGGCACCCCGGGATGTGATGTAAGAACGCCCTGCGCGTCAGCCT
GGAACGGGCTTGATTGCTGCCCTTAACTGTGTTCTGAAGTTTCACAGCTTGGCTTCAGCC
TATCAGCGCATACATTGGCATGAATTTTGTGAATTAGGGTGTTAAAAACTGGAATTGAAT
TTGTACAAAAAAAAAAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_022779 unedited
TCGCATTTTGAATACGACTCACTATAGGGCGGCCGNAATTATTGAGCTCCAGAGCCTGTT
AGGGAGCTACCCAGACAGGCTTTAGTTCCTAAAGTGCTGAGGAAACGCACGGGGCGGTAA
AGGGGAGGCTCGTCGGTGAGATGGCGCCGGATCTCGCTTCTCAGAGGCATTCAGAGAGCT
TCCCCAGCGTGAACTCTCGGCCGAATGTAATTTTGCCAGGCCGCGAGGGACGCAGAGAGG
GGCTGCCACCAGGCGGGGGCACGCGCGGTAGTCTTGTCCCTACCCGCCCGGTGCCGCCGT
CCCCGGCTCCCCTCGGGACGAGTCCGTACAGCTGGAGCCGTTCCGGACCCGGAAGGGGCG
GTGGCGCAGGGTCCTCGCGCGTCCCACGTGGGGTCCCGGGCCCAGCAGTCTGCGCACCGG
GCTCGCTGCTGCACCACGCGTCACCCACGCAGACCATGGCAGCCGCCGACGGTTCGCTCT
TCGACAACCCCAGGACGTTCTCCAGACGTCCCCCAGCCCAGGCGAGTCGGCAAGCAAAGG
CTACGAAAAGAAAATACCAAGCGTCCAGTTGAGCTCCCCCAGCGAAACCGGAGAACGAAA
CTTCATTTTTCTCCACCCAAGAAAACTAATGGTTAAGGAAACTCAAAAGGACTTTTAGGG
GGAACGCACAAAAAATGTTTTCTCCAAAGAAGCATTCGGGTAACACAAGTGATGGAAACC
CGGGGGAAAAGACGGTGCATTAAAAC
Restriction Sites Please inquire |
ACCN NM_022779
Insert Size 3140 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a good match to the protein associated to this reference except for one SNP.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022779.7, NP_073616.6
RefSeq Size 3285 bp
RefSeq ORF 2556 bp
Locus ID 64794
UniProt ID Q9H8H2
Cytogenetics 9q34.13
Summary DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. provided by RefSeq, Apr 2016
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_022779" in other vectors (6)
SKU Description Size Price
RC223495 DDX31 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1 10 ug
$782.00
RC223495L1 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, Myc-DDK-tagged 10 ug
$1,082.00
RC223495L2 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, mGFP tagged 10 ug
$1,082.00
RC223495L3 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, Myc-DDK-tagged 10 ug
$1,082.00
RC223495L4 Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1, mGFP tagged 10 ug
$1,082.00
RG223495 DDX31 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 (DDX31), transcript variant 1 10 ug
$789.00 $982.00

file-alt Citations

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