USP37 (NM_020935) Human Tagged ORF Clone

SKU RC223407

USP37 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 37 (USP37)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: USP37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223407 representing NM_020935; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTCCTCTGAAGATACATGGTCCTATCAGAATTCGAAGTATGCAGACTGGGATTACAAAGTGGAAAG; AAGGATCCTTTGAAATTGTAGAAAAAGAGAATAAAGTCAGCCTAGTAGTTCACTACAATACTGGAGGAAT; TCCAAGGATATTTCAGCTAAGTCATAACATTAAAAATGTGGTGCTTCGACCCAGTGGAGCGAAACAAAGC; CGCCTAATGTTAACTCTGCAAGATAACAGCTTCTTGTCTATTGACAAAGTACCAAGTAAGGATGCAGAGG; AAATGAGGTTGTTTCTAGATGCAGTCCATCAAAACAGACTTCCTGCAGCCATGAAACCGTCTCAGGGGTC; TGGTAGTTTTGGAGCCATTCTGGGCAGCAGGACCTCACAGAAGGAAACCAGCAGGCAGCTTTCTTACTCA; GACAATCAGGCTTCTGCAAAAAGAGGAAGTTTGGAAACTAAAGATGATATTCCATTTCGAAAAGTTCTTG; GTAATCCGGGTAGAGGATCGATTAAGACTGTAGCAGGAAGTGGAATAGCTCGGACGATTCCTTCTTTGAC; ATCTACTTCAACACCTCTTAGATCAGGGTTGCTAGAAAATCGTACTGAAAAGAGGAAAAGAATGATATCA; ACTGGCTCAGAATTGAATGAAGATTACCCTAAGGAAAATGATTCATCATCGAACAACAAGGCCATGACAG; ATCCCTCCAGAAAGTATTTAACCAGCAGTAGAGAAAAGCAGCTGAGTTTGAAACAGTCAGAAGAGAATAG; GACATCAGGGCTTTTACCTTTACAGTCATCATCCTTTTATGGTAGCAGAGCTGGATCCAAGGAACACTCT; TCTGGTGGCACTAACTTAGACAGGACTAATGTTTCAAGCCAGACTCCCTCTGCCAAAAGAAGTTTGGGAT; TTCTTCCTCAGCCAGTTCCTCTTTCTGTTAAAAAACTGAGGTGTAACCAGGATTACACTGGCTGGAATAA; ACCAAGAGTGCCCCTTTCCTCTCACCAACAGCAGCAACTGCAGGGCTTCTCCAATTTGGGAAATACCTGC; TATATGAATGCTATTCTACAATCTCTATTTTCACTCCAGTCATTTGCAAATGACTTGCTTAAACAAGGTA; TCCCATGGAAGAAAATTCCACTCAATGCACTTATCAGACGCTTTGCACACTTGCTTGTTAAAAAAGATAT; CTGTAATTCAGAGACCAAAAAGGATTTACTCAAGAAGGTTAAAAATGCCATTTCAGCTACAGCAGAGAGA; TTCTCTGGTTATATGCAGAATGATGCTCATGAATTTTTAAGTCAGTGTTTGGACCAGCTGAAAGAAGATA; TGGAAAAATTAAATAAAACTTGGAAGACTGAACCTGTTTCTGGAGAAGAAAATTCACCAGATATTTCAGC; TACCAGAGCATACACTTGCCCTGTTATTACTAATTTGGAGTTTGAGGTTCAGCACTCCATCATTTGTAAA; GCATGTGGAGAGATTATCCCCAAAAGAGAACAGTTTAATGACCTCTCTATTGACCTTCCTCGTAGGAAAA; AACCACTCCCTCCTCGTTCAATTCAAGATTCTCTTGATCTTTTCTTTAGGGCCGAAGAACTGGAGTATTC; TTGTGAGAAGTGTGGTGGGAAGTGTGCTCTTGTCAGGCACAAATTTAACAGGCTTCCTAGGGTCCTCATT; CTCCATTTGAAACGATATAGCTTCAATGTGGCTCTCTCGCTTAACAATAAGATTGGGCAGCAAGTCATCA; TTCCAAGATACCTGACCCTGTCATCTCATTGCACTGAAAATACAAAACCACCTTTTACCCTTGGTTGGAG; TGCACATATGGCAATTTCTAGACCATTGAAAGCCTCTCAAATGGTGAATTCCTGCATCACCAGCCCTTCT; ACACCTTCAAAGAAATTCACCTTCAAATCCAAGAGCTCCTTGGCTTTATGCCTTGATTCAGACAGTGAGG; ATGAGCTAAAACGTTCTGTGGCCCTCAGCCAGAGACTTTGTGAAATGTTAGGCAACGAACAGCAGCAGGA; AGACCTGGAAAAAGATTCAAAATTATGCCCAATAGAGCCTGACAAGTCTGAATTGGAAAACTCAGGATTT; GACAGAATGAGCGAAGAAGAGCTTCTAGCAGCTGTCTTGGAGATAAGTAAGAGAGATGCTTCACCATCTC; TGAGTCATGAAGATGATGATAAGCCAACTAGCAGCCCAGATACCGGATTTGCAGAAGATGATATTCAAGA; AATGCCAGAAAATCCAGACACTATGGAAACTGAGAAGCCCAAAACAATCACAGAGCTGGATCCTGCCAGT; TTTACTGAGATAACTAAAGACTGTGATGAGAATAAAGAAAACAAAACTCCAGAAGGATCTCAGGGAGAAG; TTGATTGGCTCCAGCAGTATGATATGGAGCGTGAAAGGGAAGAGCAAGAGCTTCAGCAGGCACTGGCTCA; GAGCCTTCAAGAGCAAGAGGCTTGGGAACAGAAAGAAGATGATGACCTCAAAAGAGCTACCGAGTTAAGT; CTTCAAGAGTTTAACAACTCCTTTGTGGATGCATTGGGTTCTGATGAGGACTCTGGAAATGAGGATGTTT; TTGATATGGAGTACACAGAAGCTGAAGCTGAGGAACTGAAAAGAAATGCTGAGACAGGAAATCTGCCTCA; TTCGTACCGGCTCATCAGTGTTGTCAGTCACATTGGTAGCACTTCTTCTTCAGGTCATTACATTAGTGAT; GTATATGACATTAAGAAGCAAGCGTGGTTTACTTACAATGACCTGGAGGTATCAAAAATCCAAGAGGCTG; CCGTGCAGAGTGATCGAGATCGGAGTGGCTACATCTTCTTTTATATGCACAAGGAGATCTTTGATGAGCT; GCTGGAAACAGAAAAGAACTCTCAGTCACTTAGCACGGAAGTGGGGAAGACTACCCGTCAGGCCTCG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223407 representing NM_020935; Red=Cloning site Green=Tags(s); ; MSPLKIHGPIRIRSMQTGITKWKEGSFEIVEKENKVSLVVHYNTGGIPRIFQLSHNIKNVVLRPSGAKQS; RLMLTLQDNSFLSIDKVPSKDAEEMRLFLDAVHQNRLPAAMKPSQGSGSFGAILGSRTSQKETSRQLSYS; DNQASAKRGSLETKDDIPFRKVLGNPGRGSIKTVAGSGIARTIPSLTSTSTPLRSGLLENRTEKRKRMIS; TGSELNEDYPKENDSSSNNKAMTDPSRKYLTSSREKQLSLKQSEENRTSGLLPLQSSSFYGSRAGSKEHS; SGGTNLDRTNVSSQTPSAKRSLGFLPQPVPLSVKKLRCNQDYTGWNKPRVPLSSHQQQQLQGFSNLGNTC; YMNAILQSLFSLQSFANDLLKQGIPWKKIPLNALIRRFAHLLVKKDICNSETKKDLLKKVKNAISATAER; FSGYMQNDAHEFLSQCLDQLKEDMEKLNKTWKTEPVSGEENSPDISATRAYTCPVITNLEFEVQHSIICK; ACGEIIPKREQFNDLSIDLPRRKKPLPPRSIQDSLDLFFRAEELEYSCEKCGGKCALVRHKFNRLPRVLI; LHLKRYSFNVALSLNNKIGQQVIIPRYLTLSSHCTENTKPPFTLGWSAHMAISRPLKASQMVNSCITSPS; TPSKKFTFKSKSSLALCLDSDSEDELKRSVALSQRLCEMLGNEQQQEDLEKDSKLCPIEPDKSELENSGF; DRMSEEELLAAVLEISKRDASPSLSHEDDDKPTSSPDTGFAEDDIQEMPENPDTMETEKPKTITELDPAS; FTEITKDCDENKENKTPEGSQGEVDWLQQYDMEREREEQELQQALAQSLQEQEAWEQKEDDDLKRATELS; LQEFNNSFVDALGSDEDSGNEDVFDMEYTEAEAEELKRNAETGNLPHSYRLISVVSHIGSTSSSGHYISD; VYDIKKQAWFTYNDLEVSKIQEAAVQSDRDRSGYIFFYMHKEIFDELLETEKNSQSLSTEVGKTTRQAS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020935
• ORF Size: 2937 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020935.2
• RefSeq Size: 8015 bp
• RefSeq ORF: 2940 bp
• Locus ID: 57695
• UniProt ID: Q86T82
• Cytogenetics: 2q35
• Protein Families: Protease
• MW: 110 kDa
• Summary: Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) (PubMed:21596315). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1 (PubMed:27296872).[UniProtKB/Swiss-Prot Function]