USP37 (NM_020935) Human Untagged Clone

SKU SC107387

USP37 (untagged)-Human ubiquitin specific peptidase 37 (USP37)

Product Data

• Type: Human Untagged Clone
• Target Symbol: USP37
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107387 sequence for NM_020935 edited (data generated by NextGen Sequencing); ATGTCTCCTCTGAAGATACATGGTCCTATCAGAATTCGAAGTATGCAGACTGGGATTACA; AAGTGGAAAGAAGGATCCTTTGAAATTGTAGAAAAAGAGAATAAAGTCAGCCTAGTAGTT; CACTACAATACTGGAGGAATTCCAAGGATATTTCAGCTAAGTCATAACATTAAAAATGTG; GTGCTTCGACCCAGTGGAGCGAAACAAAGCCGCCTAATGTTAACTCTGCAAGATAACAGC; TTCTTGTCTATTGACAAAGTACCAAGTAAGGATGCAGAGGAAATGAGGTTGTTTCTAGAT; GCAGTCCATCAAAACAGACTTCCTGCAGCCATGAAACCGTCTCAGGGGTCTGGTAGTTTT; GGAGCCATTCTGGGCAGCAGGACCTCACAGAAGGAAACCAGCAGGCAGCTTTCTTACTCA; GACAATCAGGCTTCTGCAAAAAGAGGAAGTTTGGAAACTAAAGATGATATTCCATTTCGA; AAAGTTCTTGGTAATCCGGGTAGAGGATCGATTAAGACTGTAGCAGGAAGTGGAATAGCT; CGGACGATTCCTTCTTTGACATCTACTTCAACACCTCTTAGATCAGGGTTGCTAGAAAAT; CGTACTGAAAAGAGGAAAAGAATGATATCAACTGGCTCAGAATTGAATGAAGATTACCCT; AAGGAAAATGATTCATCATCGAACAACAAGGCCATGACAGATCCCTCCAGAAAGTATTTA; ACCAGCAGTAGAGAAAAGCAGCTGAGTTTGAAACAGTCAGAAGAGAATAGGACATCAGGG; CTTTTACCTTTACAGTCATCATCCTTTTATGGTAGCAGAGCTGGATCCAAGGAACACTCT; TCTGGTGGCACTAACTTAGACAGGACTAATGTTTCAAGCCAGACTCCCTCTGCCAAAAGA; AGTTTGGGATTTCTTCCTCAGCCAGTTCCTCTTTCTGTTAAAAAACTGAGGTGTAACCAG; GATTACACTGGCTGGAATAAACCAAGAGTGCCCCTTTCCTCTCACCAACAGCAGCAACTG; CAGGGCTTCTCCAATTTGGGAAATACCTGCTATATGAATGCTATTCTACAATCTCTATTT; TCACTCCAGTCATTTGCAAATGACTTGCTTAAACAAGGTATCCCATGGAAGAAAATTCCA; CTCAATGCACTTATCAGACGCTTTGCACACTTGCTTGTTAAAAAAGATATCTGTAATTCA; GAGACCAAAAAGGATTTACTCAAGAAGGTTAAAAATGCCATTTCAGCTACAGCAGAGAGA; TTCTCTGGTTATATGCAGAATGATGCTCATGAATTTTTAAGTCAGTGTTTGGACCAGCTG; AAAGAAGATATGGAAAAATTAAATAAAACTTGGAAGACTGAACCTGTTTCTGGAGAAGAA; AATTCACCAGATATTTCAGCTACCAGAGCATACACTTGCCCTGTTATTACTAATTTGGAG; TTTGAGGTTCAGCACTCCATCATTTGTAAAGCATGTGGAGAGATTATCCCCAAAAGAGAA; CAGTTTAATGACCTCTCTATTGACCTTCCTCGTAGGAAAAAACCACTCCCTCCTCGTTCA; ATTCAAGATTCTCTTGATCTTTTCTTTAGGGCCGAAGAACTGGAGTATTCTTGTGAGAAG; TGTGGTGGGAAGTGTGCTCTTGTCAGGCACAAATTTAACAGGCTTCCTAGGGTCCTCATT; CTCCATTTGAAACGATATAGCTTCAATGTGGCTCTCTCGCTTAACAATAAGATTGGGCAG; CAAGTCATCATTCCAAGATACCTGACCCTGTCATCTCATTGCACTGAAAATACAAAACCA; CCTTTTACCCTTGGTTGGAGTGCACATATGGCAATTTCTAGACCATTGAAAGCCTCTCAA; ATGGTGAATTCCTGCATCACCAGCCCTTCTACACCTTCAAAGAAATTCACCTTCAAATCC; AAGAGCTCCTTGGCTTTATGCCTTGATTCAGACAGTGAGGATGAGCTAAAACGTTCTGTG; GCCCTCAGCCAGAGACTTTGTGAAATGTTAGGCAACGAACAGCAGCAGGAAGACCTGGAA; AAAGATTCAAAATTATGCCCAATAGAGCCTGACAAGTCTGAATTGGAAAACTCAGGATTT; GACAGAATGAGCGAAGAAGAGCTTCTAGCAGCTGTCTTGGAGATAAGTAAGAGAGATGCT; TCACCATCTCTGAGTCATGAAGATGATGATAAGCCAACTAGCAGCCCAGATACCGGATTT; GCAGAAGATGATATTCAAGAAATGCCAGAAAATCCAGACACTATGGAAACTGAGAAGCCC; AAAACAATCACAGAGCTGGATCCTGCCAGTTTTACTGAGATAACTAAAGACTGTGATGAG; AATAAAGAAAACAAAACTCCAGAAGGATCTCAGGGAGAAGTTGATTGGCTCCAGCAGTAT; GATATGGAGCGTGAAAGGGAAGAGCAAGAGCTTCAGCAGGCACTGGCTCAGAGCCTTCAA; GAGCAAGAGGCTTGGGAACAGAAAGAAGATGATGACCTCAAAAGAGCTACCGAGTTAAGT; CTTCAAGAGTTTAACAACTCCTTTGTGGATGCATTGGGTTCTGATGAGGACTCTGGAAAT; GAGGATGTTTTTGATATGGAGTACACAGAAGCTGAAGCTGAGGAACTGAAAAGAAATGCT; GAGACAGGAAATCTGCCTCATTCGTACCGGCTCATCAGTGTTGTCAGTCACATTGGTAGC; ACTTCTTCTTCAGGTCATTACATTAGTGATGTATATGACATTAAGAAGCAAGCGTGGTTT; ACTTACAATGACCTGGAGGTATCAAAAATCCAAGAGGCTGCCGTGCAGAGTGATCGAGAT; CGGAGTGGCTACATCTTCTTTTATATGCACAAGGAGATCTTTGATGAGCTGCTGGAAACA; GAAAAGAACTCTCAGTCACTTAGCACGGAAGTGGGGAAGACTACCCGTCAGGCCTCGTGA; ; Clone variation with respect to NM_020935.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020935 unedited; TTCCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA; GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA; ATTCGGCACGAGGCCGCCATCAGCCGCTCTCCCTGACTAGGGGTAGCTAGCGGCCTCCCT; CCTTTGGGTTCTGTATCCCTACTTCCATTACCCTCGGCTCCTCCCACTCCTCGGGGGCTC; CGTGCTTTCCGCGGGTCTGTCCGGGGGCTCCGGACCCTCGGCGCAGCTGAGTTGATGGCT; TCCGGAGAACTGGCATAGCTGCAGAATATGAGTAGTGTCCCAAGAAGAGTGCTTTGCCTT; TGGCACAAGGATCAGAATAAAGGTGAATTGCTATTACATAGGGTTTTTCGGTAAAAGTCA; CTGAAAATTATTTGCCATACAGTAACCAGAGACCTCCAACTAGGGGCCCCCAAACTGTAT; CCTGCCTGTAGCCAGAAGCAATTTTTAAAGAAAATATGTCTCCTCTGAAGATACATGGTC; CTATCAGAATTCGAAGTATGCAGACTGGGATTACAAAGTGGAAAGAAGGATCCTTTGAAA; TTGTAGAAAAAGAGAATAAGTCAAGCTAGTAGTTCACTACAATACTGGAGGGAATTCCAG; GATAATTCAGCTAAGGCATAACATTAAAAAGGTGGTGCTTCAACCCAGTGGAGCGAAACA; AAAGCCGCCTATGGTAACCTCTGCAGAAACCAGCTTCTTGCTTATTGCCAAAGTACCCCA; GTAGGATGCCCACGGAAATGAGGTTGTTTCTAGAAGCAGTCCATTAAAACAGACTTCCTG; CAGCCATGAAACCGTCTAAGGGTCTGGAAGTTTTGGGGCCTTCTGGGCCGCGGGACCCCC; CCGAGGAAACCCCCCGGCAGCTTTTTACTCCACAATCAGGCTCTGGCCAAAAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020935 unedited; AGAAATTCCCTCATGTGATGGTGGGTTTTTAAATGTTTTTTCCTTCTGAAAACCTGATGT; GGCTTTGTCCCTCCCCCTGATATCTTTTGAATAATCCCCTTGTTTATCATAAAAGAAAAA; ACACAAAAATTCCCTCGTGGNNNCACTTTTCNATTTTTTTCTCTGGGTGATCCTAATGAT; ATCTGGGCTCACAAAATTATTTTGTGGACAGAATTTTCTCCTCCAAAGAATTCTTTATCT; GGCTTCATAAGCTGCCCACCCTAGCTGGTTACGTCCCCTTAGCGACTAGCCTANCACTTA; CTGTTGCCGCTCCTCTTTGCCTCCCCTCAGAGCACCCTTCTTGCTCCTCACTTAATTATG; CACCTCTGTCATCTGTTAGTTTTTGGGATTACCTCCGCCCATCCCCTTTTATTTGCCACC; CTCCTTTAAGGGCTTAGCCCGACAGTGTGTGGCTNAATGCCCAAGGTTTGTCCCTTNGGC; TCTGTACTTACCTTCCCACTTGCCCTCNGGCCTCTTTTCTCCTGTGGTCCCGAATTTTAG; CTTTCCCCTCCCAACCCTCCTCCTTATCCTCCCTCTGTTTGGCCGACCGATATTGCTATC; TTCTTCCCTTTCCCTATCTATTCCTGCTGGGGGTTTTCATTTGGGGCTATCTTTCCTCCC; CAATTTGTTTTACGTCTTTGGCGCCCCCCCTCTTTTGTCCTGCCTGCCTCCCGGGTTGTT; TGCCCCCGCAGATAAGAGTTCCTTCGTTCCCGCCCCTGTTTTCGCGTACGGCCCTGCAGG; TATCTCCCCGTGGGACCAACCCCCCAAAAAAAAAAAGAAAAACAGAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_020935
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020935.1, NP_065986.1
• RefSeq Size: 8015 bp
• RefSeq ORF: 2940 bp
• Locus ID: 57695
• UniProt ID: Q86T82
• Cytogenetics: 2q35
• Protein Families: Protease
• Summary: Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) (PubMed:21596315). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1 (PubMed:27296872).[UniProtKB/Swiss-Prot Function]