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PPA2 (NM_176869) Human Tagged ORF Clone

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SKU
RC223311
PPA2 (Myc-DDK-tagged)-Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PPA2 or NM_176869"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPA2
Synonyms HSPC124; SCFAI; SCFI; SID6-306
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223311 representing NM_176869
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGCGCTGCTGCGGCTGCTGCGCACGGGTGCCCCAGCCGCTGCGTGCCTGCGGTTGGGGACCAGTG
CAGGGACCGGGTCGCGCCGTGCTATGGCCCTGTACCACACTGAGGAGCGCGGCCAGCCCTGCTCGCAGAA
TTACCGCCTCTTCTTTAAGAATGTAACTGGTCACTACATTTCCCCCTTTCATGATATTCCTCTGAAGGTG
AACTCTAAAGAGGAAAATGGCATTCCTATGAAGAAAGCACGAAATGATGAATATGAGAATCTGTTTAATA
TGATTGTAGAAATACCTCGGTGGACAAATGCTAAAATGGAGATTGCCACCAAGGAGCCAATGAATCCCAT
TAAACAATATGTAAAGGATGGAAAGCTACGCTATGTGGCGAATATCTTCCCTTACAAGGGTTATATATGG
AATTATGGTACCCTCCCTCAGACTTGGGAAGATCCCCATGAAAAAGATAAGAGCACGAACTGCTTTGGAG
ATAATGATCCTATTGATGTTTGCGAAATAGGCTCAAAGATTCTTTCTTGTGGAGAAGTTATTCATGTGAA
GATCCTTGGAATTTTGGCTCTTATTGATGAAGGTGAAACAGATTGGAAATTAATTGCTATCAATGCGAAT
GATCCTGAAGCCTCAAAGTTTCATGATATTGATGATGTTAAGAAGTTCAAACCGGGTTACCTGGAAGCTA
CTCTTAATTGGTTTAGATTATATAAGGTACCAGATGGAAAACCAGAAAACCAGTTTGCTTTTAATGGAGA
ATTCAAAAACAAGGCTTTTGCTCTTGAAGTTATTAAATCCACTCATCAATGTTGGAAAGCATTGCTTATG
AAGAACTGTAATGGAGGAGCTATAAATTGCACAAACGTGCAGATATCTGATAGCCCTTTCCGTTGCACTC
AAGAGGAAGCAAGATCATTAGTTGAATCGGTATCATCTTCACCAAATAAAGAAAGTAATGAAGAAGAGCA
AGTGTGGCACTTCCTTGGCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223311 representing NM_176869
Red=Cloning site Green=Tags(s)

MSALLRLLRTGAPAAACLRLGTSAGTGSRRAMALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKV
NSKEENGIPMKKARNDEYENLFNMIVEIPRWTNAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIW
NYGTLPQTWEDPHEKDKSTNCFGDNDPIDVCEIGSKILSCGEVIHVKILGILALIDEGETDWKLIAINAN
DPEASKFHDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLM
KNCNGGAINCTNVQISDSPFRCTQEEARSLVESVSSSPNKESNEEEQVWHFLGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_176869
ORF Size 1002 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_176869.3
RefSeq Size 1682 bp
RefSeq ORF 1005 bp
Locus ID 27068
UniProt ID Q9H2U2
Cytogenetics 4q24
Protein Pathways Oxidative phosphorylation
MW 34.8 kDa
Summary The protein encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. provided by RefSeq, Jul 2008
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"NM_176869" in other vectors (6)
SKU Description Size Price
RC223311L1 Lenti ORF clone of Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC223311L2 Lenti ORF clone of Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$757.00
RC223311L3 Lenti ORF clone of Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC223311L4 Lenti ORF clone of Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$757.00
RG223311 PPA2 (tGFP-tagged) - Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$657.00
SC307076 PPA2 (untagged)-Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$503.00

Plasmid Map for RC223311

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223311
Circular map for RC223311

file-alt Citations

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