CCR5 (NM_000579) Human Tagged ORF Clone
SKU
RC223291
CCR5 (Myc-DDK-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CCR5 |
| Synonyms | CC-CKR-5; CCCKR5; CCR-5; CD195; CKR-5; CKR5; CMKBR5; IDDM22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000579 |
| ORF Size | 1056 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000579.3, NP_000570.1 |
| RefSeq Size | 3655 bp |
| RefSeq ORF | 1059 bp |
| Locus ID | 1234 |
| UniProt ID | P51681, Q38L21 |
| Cytogenetics | 3p21.31 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane |
| Protein Pathways | Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis |
| MW | 40.3 kDa |
| Summary | This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Jul 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000579" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC223291L1 | Lenti-ORF clone of CCR5 (Myc-DDK-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A | 10 ug |
$986.00
|
|
| RC223291L2 | Lenti-ORF clone of CCR5 (mGFP-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A | 10 ug |
$986.00
|
|
| RC223291L3 | Lenti-ORF clone of CCR5 (Myc-DDK-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A | 10 ug |
$986.00
|
|
| RC223291L4 | Lenti-ORF clone of CCR5 (mGFP-tagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A | 10 ug |
$986.00
|
|
| RG223291 | CCR5 (tGFP-tagged) - Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A | 10 ug |
$886.00
|
|
| SC110858 | CCR5 (untagged)-Human chemokine (C-C motif) receptor 5 (CCR5), transcript variant A | 10 ug |
$686.00
|
|
Citations
Microglial-to-neuronal CCR5 signaling regulates autophagy in neurodegeneration
Festa, Siddiqi, Jimenez-Sanchez et al
Neuron (2023) 111 (13), 2021-2037.e12 DOI: 10.1016/j.neuron.2023.04.006
Festa, Siddiqi, Jimenez-Sanchez et al
Neuron (2023) 111 (13), 2021-2037.e12 DOI: 10.1016/j.neuron.2023.04.006
Cited by 126
AI Summary: In this study, the OriGene pCMV6-CCR5-Myc-FLAG plasmid (RC223291) was used to investigate the role of CCR5 in neuronal autophagy regulation during neurodegeneration.
Snippet: The following DNA constructs were used in this study: pHM6-httQ74-HA,50 pEGFP-empty (Invitrogen), pEGFP-ATG16L1 (gift from N.Mizushima),51 pEGFP-ATG5 (gift from N.Mizushima),52 pCMV6-CCR5-Myc-FLAG (Origene RC223291). The following pre-designed siRNAs (ON-TARGETplus SMARTpool from GE Healthcare Dharmacon) were used in this study: control non-targeting siRNA (D-001810-10);CCR5 Smart Pool (L-004855-00-0005), RAPTOR Smart Pool (L-004107-00-0005), TSC2 Smart Pool (L-003029-00-0005), ATG7 Smart Pool (L-020112-00-0005), ATG10 Smart Pool (L-019426-01-0005)
Immunotherapy targeting the C-terminal domain of TDP-43 decreases neuropathology and confers neuroprotection in mouse models of ALS/FTD
Afroz, Chevalier, Audrain et al
Neurobiol Dis (2023) 179, 106050 DOI: 10.1016/j.nbd.2023.106050
Afroz, Chevalier, Audrain et al
Neurobiol Dis (2023) 179, 106050 DOI: 10.1016/j.nbd.2023.106050
Cited by 36
AI Summary: OriGene’s recombinant full-length human TDP-43 protein was used to engineer a vaccine by conjugation to liposomes, enabling the study of immunotherapy targeting TDP-43 pathology in ALS/FTD mouse models.
Snippet: Effective therapies are urgently needed to safely target TDP-43 pathology as it is closely associated with the onset and development of devastating diseases such as Save;Vaccine was engineered by conjugation of recombinant full-length human TDP-43 (Origene) on the surface of SupraAntigen liposomes. Monophosphoryl Hexa-acyl Lipid A, 3-Deacyl (Synthetic) (3D-(6-acyl) PHAD ) was used as adjuvant. ...
Showing 1-2 of 2 papers.
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