DMRT2 (NM_181872) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC223278
DMRT2 (Myc-DDK-tagged)-Human doublesex and mab-3 related transcription factor 2 (DMRT2), transcript variant 3
  $783.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DMRT2
Synonyms DSXL-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223278 representing NM_181872
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGACCCGCAGGCTGGCTCCGCGGCCGGGGACTGGGAGATCGATGTCGAGAGCCTGGAGCTGGAAG
AGGACGTCTGCGGGGCGCCGCGGTCCACGCCCCCCGGGCCCAGCCCGCCGCCGGCGGACGGGGACTGCGA
GGACGACGAAGATGACGACGGGGTGGACGAAGACGCGGAAGAAGAGGGCGACGGCGAGGAGGCAGGCGCG
TCCCCCGGGATGCCCGGCCAGCCGGAGCAGCGGGGGGGACCGCAGCCGAGGCCGCCGCTCGCGCCTCAGG
CCTCACCCGCCGGCACCGGTCCCCGAGAGCGCTGCACTCCCGCGGGCGGCGGCGCGGAGCCGCGCAAGCT
GAGCCGCACGCCCAAGTGCGCGCGCTGCCGCAACCACGGCGTGGTGTCCTGCCTGAAGGGCCACAAGCGC
TTCTGTCGCTGGCGCGACTGCCAGTGCGCCAACTGCCTGCTGGTGGTGGAGCGGCAGCGCGTCATGGCCG
CCCAGGTGGCGCTCCGGAGGCAGCAGGCCACCGAGGACAAGAAGGGGCTTTCCGGGAAACAGAATAATTT
CGAGCGCAAAGCTGTGTACCAGAGGCAAGTCAGAGCCCCCAGTTTGCTGGCCAAAAGCATTTTAGAAGGC
TATCGCCCCATTCCAGCGGAGACTTATGTAGGAGGGACCTTCCCTCTACCTCCCCCAGTTAGTGACAGGA
TGAGGAAAAGAAGAGCCTTTGCTGATAAAGAGTTGGAGAACATTATGCTGGAGAGAGAATATAAAGAAAG
GGAGATGTTGGAAACTTCTCAAGCTGCTGCTCTGTTTCTGCCCAACCGCATGGTGCCTGGACCTGACTAC
AATTCCTACAAAAGTGCCTACAGCCCCAGCCCAGTGGAACCACCAAGCAAGGACTTCTGTAATTTTTTGC
CCACCTGCCTTGATTTAACCATGCAGTATTCAGGGTCTGGGAATATGGAACTAATTTCTTCTAATGTCAG
CGTGGGCACAACTTATAGACAGTATCCCTTGTCCTCAAGATTTTTAGTTTGGCCCAAGTGTGGCCCCATT
AGCGACACCCTCCTCTACCAGCAATGCCTGCTAAATGCCACCACCTCAGTTCAAGCCCTGAAGCATGGGG
CCAGCTGGGACTTGAAGGGAGCACGAGTCCAGGATGGACTCAGTGCAGAGCAGGACATGATGCCATCGAA
ATTGGAAGGTTCCCTGGTGCTGCCTCACACTCCTGAGATCCAGACCACGAGAAGTGACCTTCAGGGTCAT
CAGGCTGTCCCAGAGAGGTCCGCGTTCTCCCCACCCCGACGGAATTTCTCTCCCATTGTTGACACGGACT
CCCTGGCAGCTCAAGGGCATGTCTTAACGAAGATCAGCAAAGAAAACACCAGGCACCCTCTGCCACTTAG
ACATAATCCATTCCACTCATTATTCCAGCAAACACTTACTGACAAATCGGGTCCTGAGTTGAAAACACCA
TTTGTCAAAGAGGCCTTTGAAGAGACCCCTAAGAAACACAGAGAGTGTTTAGTTAAGGACAACCAGAAGT
ACACATTTACAATAGATAGATGTGCAAAAGACCTTTTTGTAGCCAAACAAGTTGGAACAAAACTCTCGGT
GAATGAACCACTGTCATTTTCTGTTGAGTCTATTCTTAAGAGGCCTTCATCTGCCATCACTCGTGTCTCT
CAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC223278 representing NM_181872
Red=Cloning site Green=Tags(s)

MADPQAGSAAGDWEIDVESLELEEDVCGAPRSTPPGPSPPPADGDCEDDEDDDGVDEDAEEEGDGEEAGA
SPGMPGQPEQRGGPQPRPPLAPQASPAGTGPRERCTPAGGGAEPRKLSRTPKCARCRNHGVVSCLKGHKR
FCRWRDCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLSGKQNNFERKAVYQRQVRAPSLLAKSILEG
YRPIPAETYVGGTFPLPPPVSDRMRKRRAFADKELENIMLEREYKEREMLETSQAAALFLPNRMVPGPDY
NSYKSAYSPSPVEPPSKDFCNFLPTCLDLTMQYSGSGNMELISSNVSVGTTYRQYPLSSRFLVWPKCGPI
SDTLLYQQCLLNATTSVQALKHGASWDLKGARVQDGLSAEQDMMPSKLEGSLVLPHTPEIQTTRSDLQGH
QAVPERSAFSPPRRNFSPIVDTDSLAAQGHVLTKISKENTRHPLPLRHNPFHSLFQQTLTDKSGPELKTP
FVKEAFEETPKKHRECLVKDNQKYTFTIDRCAKDLFVAKQVGTKLSVNEPLSFSVESILKRPSSAITRVS
Q

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181872
ORF Size 1683 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181872.1, NP_870987.1
RefSeq Size 1969 bp
RefSeq ORF 1686 bp
Locus ID 10655
UniProt ID Q9Y5R5, Q05C20
Cytogenetics 9p24.3
Protein Families Transcription Factors, Transmembrane
MW 61.6 kDa
Summary The protein encoded by this gene belongs to the DMRT gene family, sharing a DM DNA-binding domain with Drosophila 'doublesex' (dsx) and C. elegans mab3, genes involved in sex determination in these organisms. Also, this gene is located in a region of the human genome (chromosome 9p24.3) associated with gonadal dysgenesis and XY sex reversal. Hence this gene is one of the candidates for sex-determining gene(s) on chr 9. provided by RefSeq, Apr 2010
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_181872" in other vectors (4)
SKU Description Size Price
RC223278L3 Lenti ORF clone of Human doublesex and mab-3 related transcription factor 2 (DMRT2), transcript variant 3, Myc-DDK-tagged 10 ug
$1,083.00
RC223278L4 Lenti ORF clone of Human doublesex and mab-3 related transcription factor 2 (DMRT2), transcript variant 3, mGFP tagged 10 ug
$1,083.00
RG223278 DMRT2 (tGFP-tagged) - Human doublesex and mab-3 related transcription factor 2 (DMRT2), transcript variant 3 10 ug
$983.00
SC307390 DMRT2 (untagged)-Human doublesex and mab-3 related transcription factor 2 (DMRT2), transcript variant 3 10 ug
$784.00

Plasmid Map for RC223278

Download sequence for

Cloning scheme for RC223278

Circular map for RC223278

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products