BTN2A2 (NM_181531) Human Tagged ORF Clone

  • TrueORF®
SKU
RC223245
BTN2A2 (Myc-DDK-tagged)-Human butyrophilin, subfamily 2, member A2 (BTN2A2), transcript variant 2
$289.00 $457.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BTN2A2
Synonyms BT2.2; BTF2; BTN2.2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223245 representing NM_181531
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACCAGCTGCTGCTCTGCACTTCTCCCTGCCAGCCTCCCTCCTCCTCCTCCTGCTCCTCCTCCTTC
TCAGCCTGTGTGCACTGGTCTCAGGCCTTGGGTCTAAGCCCCTCATTGAAATCAAGGCCCAAGAGGATGG
GAGCATCTGGCTGGAGTGCATATCTGGAGGGTGGTACCCAGAGCCCCTCACAGTGTGGAGGGACCCCTAC
GGTGAGGTTGTGCCCGCCCTGAAGGAGGTTTCCATCGCTGATGCTGACGGCCTCTTCATGGTCACCACAG
CTGTGATCATCAGAGACAAGTATGTGAGGAATGTGTCCTGCTCTGTCAACAACACCCTGCTCGGCCAGGA
GAAGGAAACTGTCATTTTTATTCCAGAATCCTTTATGCCCAGCGCATCTCCCTGGATGGTGGCCCTAGCT
GTCATCCTGACCGCATCTCCCTGGATGGTGTCCATGACTGTCATCCTGGCTGTTTTCATCATCTTCATGG
CTGTCAGCATCTGTTGCATCAAGAAACTTCAAAGGGAAAAAAAGATTCTGTCAGGGGAAAAGAAAGTTGA
ACAAGAGGAAAAAGAAATTGCACAGCAACTTCAAGAAGAATTGCGATGGAGAAGAACATTCTTACATGCT
GCTGATGTGGTCCTGGATCCAGACACCGCTCATCCCGAGCTCTTCCTGTCAGAGGACCGGAGAAGTGTGA
GGCGGGGCCCCTACAGGCAGAGAGTGCCTGACAACCCAGAGAGATTCGACAGTCAGCCTTGTGTCCTGGG
ATGGGAGAGCTTCGCCTCAGGGAAACATTACTGGGAGGTGGAGGTGGAAAACGTGATGGTGTGGACTGTG
GGGGTCTGCAGACACAGTGTTGAGAGGAAAGGGGAGGTCCTGCTGATTCCTCAGAATGGCTTCTGGACCC
TGGAGATGTTTGGAAACCAATACCGGGCCCTGTCCTCCCCTGAGAGGATTCTCCCTTTGAAGGAGTCCCT
TTGCCGGGTGGGCGTCTTCCTGGACTATGAAGCTGGAGATGTCTCCTTCTACAACATGAGGGACAGATCG
CACATCTACACATGTCCCCGTTCAGCCTTTACTGTGCCTGTGAGGCCCTTCTTCAGGTTAGGGTCTGATG
ACAGCCCCATCTTCATCTGCCCTGCACTCACAGGAGCCAGTGGGGTCATGGTGCCTGAAGAGGGCCTGAA
ACTTCACAGAGTGGGGACCCACCAGAGCCTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC223245 representing NM_181531
Red=Cloning site Green=Tags(s)

MEPAAALHFSLPASLLLLLLLLLLSLCALVSGLGSKPLIEIKAQEDGSIWLECISGGWYPEPLTVWRDPY
GEVVPALKEVSIADADGLFMVTTAVIIRDKYVRNVSCSVNNTLLGQEKETVIFIPESFMPSASPWMVALA
VILTASPWMVSMTVILAVFIIFMAVSICCIKKLQREKKILSGEKKVEQEEKEIAQQLQEELRWRRTFLHA
ADVVLDPDTAHPELFLSEDRRSVRRGPYRQRVPDNPERFDSQPCVLGWESFASGKHYWEVEVENVMVWTV
GVCRHSVERKGEVLLIPQNGFWTLEMFGNQYRALSSPERILPLKESLCRVGVFLDYEAGDVSFYNMRDRS
HIYTCPRSAFTVPVRPFFRLGSDDSPIFICPALTGASGVMVPEEGLKLHRVGTHQSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181531
ORF Size 1221 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181531.3
RefSeq Size 3226 bp
RefSeq ORF 1224 bp
Locus ID 10385
UniProt ID Q8WVV5
Cytogenetics 6p22.2
Protein Families Druggable Genome, Transmembrane
MW 45.6 kDa
Summary Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2010
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Documents
Resources
"NM_181531" in other vectors (4)
SKU Description Size Price
RC223245L3 Lenti-ORF clone of BTN2A2 (Myc-DDK-tagged)-Human butyrophilin, subfamily 2, member A2 (BTN2A2), transcript variant 2 10 ug
$757.00
RC223245L4 Lenti-ORF clone of BTN2A2 (mGFP-tagged)-Human butyrophilin, subfamily 2, member A2 (BTN2A2), transcript variant 2 10 ug
$757.00
RG223245 BTN2A2 (tGFP-tagged) - Human butyrophilin, subfamily 2, member A2 (BTN2A2), transcript variant 2 10 ug
$489.00 $657.00
SC127660 BTN2A2 (untagged)-Human butyrophilin, subfamily 2, member A2 (BTN2A2), transcript variant 2 10 ug
$457.00

Plasmid Map for RC223245

Download sequence for

Cloning scheme for RC223245

Circular map for RC223245

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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