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C1R (NM_001733) Human Tagged ORF Clone

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SKU
RC223007
C1R (Myc-DDK-tagged)-Human complement component 1, r subcomponent (C1R)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $646.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C1R
Synonyms EDSPD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223007 representing NM_001733
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCTCTTGTACCTCCTGGTGCCGGCCCTGTTCTGCAGGGCAGGAGGCTCCATTCCCATCCCTCAGA
AGTTATTTGGGGAGGTGACTTCCCCTCTGTTCCCCAAGCCTTACCCCAACAACTTTGAAACAACCACTGT
GATCACAGTCCCCACGGGATACAGGGTGAAGCTCGTCTTCCAGCAGTTTGACCTGGAGCCTTCTGAAGGC
TGCTTCTATGATTATGTCAAGATCTCTGCTGATAAGAAAAGCCTGGGGAGGTTCTGTGGGCAACTGGGTT
CTCCACTGGGCAACCCCCCGGGAAAGAAGGAATTTATGTCCCAAGGGAACAAGATGCTGCTGACCTTCCA
CACAGACTTCTCCAACGAGGAGAATGGGACCATCATGTTCTACAAGGGCTTCCTGGCCTACTACCAAGCT
GTGGACCTTGATGAATGTGCTTCCCGGAGCAAATCAGGGGAGGAGGATCCCCAGCCCCAGTGCCAGCACC
TGTGTCACAACTACGTTGGAGGCTACTTCTGTTCCTGCCGTCCAGGCTATGAGCTTCAGGAAGACAGGCA
TTCCTGCCAGGCTGAGTGCAGCAGCGAGCTGTACACGGAGGCATCAGGCTACATCTCCAGCCTGGAGTAC
CCTCGGTCCTACCCCCCTGACCTGCGCTGCAACTACAGCATCCGGGTGGAGCGGGGCCTCACCCTGCACC
TCAAGTTCCTGGAGCCTTTTGATATTGATGACCACCAGCAAGTACACTGCCCCTATGACCAGCTACAGAT
CTATGCCAACGGGAAGAACATTGGCGAGTTCTGTGGGAAGCAAAGGCCCCCCGACCTCGACACCAGCAGC
AATGCTGTGGATCTGCTGTTCTTCACAGATGAGTCGGGGGACAGCCGGGGCTGGAAGCTGCGCTACACCA
CCGAGATCATCAAGTGCCCCCAGCCCAAGACCCTAGACGAGTTCACCATCATCCAGAACCTGCAGCCTCA
GTACCAGTTCCGTGACTACTTCATTGCTACCTGCAAGCAAGGCTACCAGCTCATAGAGGGGAACCAGGTG
CTGCATTCCTTCACAGCTGTCTGCCAGGATGATGGCACGTGGCATCGTGCCATGCCCAGATGCAAGATCA
AGGACTGTGGGCAGCCCCGAAACCTGCCTAATGGTGACTTCCGTTACACCACCACAATGGGAGTGAACAC
CTACAAGGCCCGTATCCAGTACTACTGCCATGAGCCATATTACAAGATGCAGACCAGAGCTGGCAGCAGG
GAGTCTGAGCAAGGGGTGTACACCTGCACAGCACAGGGCATTTGGAAGAATGAACAGAAGGGAGAGAAGA
TTCCTCGGTGCTTGCCAGTGTGTGGGAAGCCCGTGAACCCCGTGGAACAGAGGCAGCGCATCATCGGAGG
GCAAAAAGCCAAGATGGGCAACTTCCCCTGGCAGGTGTTCACCAACATCCACGGGCGCGGGGGCGGGGCC
CTGCTGGGCGACCGCTGGATCCTCACAGCTGCCCACACCCTGTATCCCAAGGAACACGAAGCGCAAAGCA
ACGCCTCTTTGGATGTGTTCCTGGGCCACACAAATGTGGAAGAGCTCATGAAGCTAGGAAATCACCCCAT
CCGCAGGGTCAGCGTCCACCCGGACTACCGTCAGGATGAGTCCTACAATTTTGAGGGGGACATCGCCCTG
CTGGAGCTGGAAAATAGTGTCACCCTGGGTCCCAACCTCCTCCCCATCTGCCTCCCTGACAACGATACCT
TCTACGACCTGGGCTTGATGGGCTATGTCAGTGGCTTCGGGGTCATGGAGGAGAAGATTGCTCATGACCT
CAGGTTTGTCCGTCTGCCCGTAGCTAATCCACAGGCCTGTGAGAACTGGCTCCGGGGAAAGAATAGGATG
GATGTGTTCTCTCAAAACATGTTCTGTGCTGGACACCCATCTCTAAAGCAGGACGCCTGCCAGGGGGATA
GTGGGGGCGTTTTTGCAGTAAGGGACCCGAACACTGATCGCTGGGTGGCCACGGGCATCGTGTCCTGGGG
CATCGGGTGCAGCAGGGGCTATGGCTTCTACACCAAAGTGCTCAACTACGTGGACTGGATCAAGAAAGAG
ATGGAGGAGGAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223007 representing NM_001733
Red=Cloning site Green=Tags(s)

MWLLYLLVPALFCRAGGSIPIPQKLFGEVTSPLFPKPYPNNFETTTVITVPTGYRVKLVFQQFDLEPSEG
CFYDYVKISADKKSLGRFCGQLGSPLGNPPGKKEFMSQGNKMLLTFHTDFSNEENGTIMFYKGFLAYYQA
VDLDECASRSKSGEEDPQPQCQHLCHNYVGGYFCSCRPGYELQEDRHSCQAECSSELYTEASGYISSLEY
PRSYPPDLRCNYSIRVERGLTLHLKFLEPFDIDDHQQVHCPYDQLQIYANGKNIGEFCGKQRPPDLDTSS
NAVDLLFFTDESGDSRGWKLRYTTEIIKCPQPKTLDEFTIIQNLQPQYQFRDYFIATCKQGYQLIEGNQV
LHSFTAVCQDDGTWHRAMPRCKIKDCGQPRNLPNGDFRYTTTMGVNTYKARIQYYCHEPYYKMQTRAGSR
ESEQGVYTCTAQGIWKNEQKGEKIPRCLPVCGKPVNPVEQRQRIIGGQKAKMGNFPWQVFTNIHGRGGGA
LLGDRWILTAAHTLYPKEHEAQSNASLDVFLGHTNVEELMKLGNHPIRRVSVHPDYRQDESYNFEGDIAL
LELENSVTLGPNLLPICLPDNDTFYDLGLMGYVSGFGVMEEKIAHDLRFVRLPVANPQACENWLRGKNRM
DVFSQNMFCAGHPSLKQDACQGDSGGVFAVRDPNTDRWVATGIVSWGIGCSRGYGFYTKVLNYVDWIKKE
MEEED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001733
ORF Size 2115 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001733.7
RefSeq Size 2526 bp
RefSeq ORF 2118 bp
Locus ID 715
UniProt ID P00736
Cytogenetics 12p13.31
Domains CCP, CUB, EGF, EGF_CA, Tryp_SPc
Protein Families Druggable Genome, Protease
Protein Pathways Complement and coagulation cascades, Systemic lupus erythematosus
MW 80 kDa
Summary This gene encodes a member of the peptidase S1 protein family. The encoded protein is a proteolytic subunit in the complement system C1 complex. The complement system acts as a mediator in the innate immune response by ultimately triggering phagocytosis, inflammation, and rupturing the bacterial cell wall. Mutations in this gene are associated with Ehlers-Danlos Syndrome. provided by RefSeq, Dec 2018
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"NM_001733" in other vectors (6)
SKU Description Size Price
RC223007L1 Lenti ORF clone of Human complement component 1, r subcomponent (C1R), Myc-DDK-tagged 10 ug
$946.00
RC223007L2 Lenti ORF clone of Human complement component 1, r subcomponent (C1R), mGFP tagged 10 ug
$946.00
RC223007L3 Lenti ORF clone of Human complement component 1, r subcomponent (C1R), Myc-DDK-tagged 10 ug
$946.00
RC223007L4 Lenti ORF clone of Human complement component 1, r subcomponent (C1R), mGFP tagged 10 ug
$946.00
RG223007 C1R (tGFP-tagged) - Human complement component 1, r subcomponent (C1R) 10 ug
$789.00 $846.00
SC119070 C1R (untagged)-Human complement component 1, r subcomponent (C1R) 10 ug
$646.00

Plasmid Map for RC223007

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223007
Circular map for RC223007

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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