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PIGN (NM_012327) Human Tagged ORF Clone

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SKU
RC222950
PIGN (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $852.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PIGN
Synonyms MCAHS; MCAHS1; MCD4; MDC4; PIG-N
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222950 representing NM_012327
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTGTTCTTTACTTTGGGATTGCTTATACATTTTGTGTTCTTCGCCTCCATCTTTGACATTTATT
TTACATCTCCTTTGGTTCATGGAATGACTCCTCAGTTTACACCATTGCCTCCTCCAGCGAGAAGATTAGT
GTTGTTTGTTGCTGATGGCCTTCGAGCAGATGCACTTTACGAATTAGATGAAAATGGAAACTCTAGAGCA
CCGTTTATTAGGAATATCATAATGCATGAAGGCAGCTGGGGCATATCTCATACACGTGTGCCAACAGAAT
CTCGGCCAGGTCATGTAGCTCTGATAGCTGGGTTTTATGAAGATGTCAGTGCAGTTGCCAAAGGATGGAA
GGAAAATCCTGTAGAGTTTGATTCTCTTTTTAATGAAAGTAAATACACATGGAGCTGGGGAAGCCCAGAT
ATCCTGCCTATGTTTGCCAAAGGTGCTAGTGGAGACCACGTTTATACATATAGTTATGATGCTAAAAGAG
AGGATTTTGGTGCTCAAGATGCAACAAAACTGGATACGTGGGTTTTTGATAATGTTAAGGACTTCTTTCA
TCATGCCAGAAACAACCAGTCTTTGTTTTCTAAAATAAATGAAGAGAAAATAGTTTTTTTCTTACATTTA
TTAGGAATAGATACAAACGGACATGCTCATCGACCATCCTCGAGAGACTACAAGGACAATATTAAAAAAG
TTGATGATGGAGTTAAAGAAATCGTGTCTATGTTTAACCATTTCTATGGAAATGATGGGAAAACAACATT
TATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCTTCAGAGACTTTA
ACTCCTTTAGTCACTTGGGGAGCTGGAATCAAGTATCCCCAAAGAGTATCAGCTCAGCAATTTGATGATG
CATTTTTGAAAGAGTGGAGATTGGAGAACTGGAAGAGGCTAGATGTCAATCAGGCTGATATTGCACCATT
GATGACTTCCCTTATTGGAGTTCCCTTTCCTCTTAACTCAGTGGGAATCCTTCCTGTGGATTATCTTAAC
AACACTGATCTCTTCAAAGCAGAGAGCATGTTTACAAATGCAGTACAGATTCTTGAACAGTTCAAGGTGA
AAATGACTCAGAAGAAAGAAGTTACTTTACCATTTTTGTTTACACCATTTAAACTGCTTTCTGATTCCAA
ACAGTTCAACATTTTAAGAAAAGCAAGATCTTATATAAAACACAGAAAGTTTGACGAAGTGGTCTCCCTT
TGCAAGGAGCTAATTCATCTTGCATTGAAAGGATTGTCCTATTATCACACATATGACAGATTCTTTTTGG
GCGTCAATGTTGTTATTGGTTTTGTGGGATGGATATCTTATGCCTCCTTGTTGATCATCAAGTCTCATTC
CAACCTTATAAAAGGTGTTAGTAAAGAAGTGAAGAAACCAAGCCATCTCCTGCCTTGTAGTTTTGTAGCT
ATTGGCATTTTAGTAGCATTTTTTCTGCTGATTCAAGCCTGTCCCTGGACATATTATGTATATGGTTTGT
TGCCACTGCCAATATGGTATGCGGTTCTAAGAGAATTTCAAGTTATTCAGGACCTTGTTGTATCAGTGTT
GACCTATCCTCTGAGCCATTTTGTTGGGTACCTGTTAGCCTTTACCCTGGGAATTGAAGTATTAGTTCTC
AGTTTTTTCTACCGCTATATGCTTACCGCTGGACTTACTGCCTTTGCAGCTTGGCCATTTCTCACTCGGC
TATGGACTCGAGCAAAGATGACCTCACTGAGTTGGACTTTCTTCTCTTTGCTCCTGGCAGTGTTCCCACT
GATGCCGGTTGTAGGTCGAAAGCCAGACATCTTTCTAGTGATGGGTGCAGGCTTGCTGGTTCTTCTGTTA
TCCCTGTGTGTTGTAACATCTCTCATGAAAAGAAAAGATAGCTTTATAAAGGAAGAGCTATTGGTACATC
TATTACAGGTGCTGAGCACAGTGCTCTCCATGTATGTTGTGTATAGCACCCAGAGTAGTCTACTCAGGAA
GCAAGGACTGCCTCTCATGAATCAAATTATTAGCTGGGCAACATTAGCCTCTTCCTTGGTTGTGCCACTA
CTGAGTTCTCCAGTTCTCTTTCAGCGATTGTTCAGCATACTTCTTTCATTGATGTCAACCTACCTACTTC
TAAGCACAGGGTATGAAGCTCTCTTTCCACTAGTGTTGTCTTGTTTGATGTTTGTCTGGATAAACATAGA
ACAAGAAACTCTACAACAATCTGGTGTTTGCTGTAAACAAAAGCTCACCAGTATCCAGTTCTCTTATAAT
ACTGATATAACTCAGTTTCGACAGCTATATCTGGATGACATCCGTAGGGCCTTTTTCCTTGTTTTCTTCT
TAGTGACAGCATTTTTTGGAACTGGAAATATAGCTTCTATTAACAGCTTTGATCTTGCCTCTGTCTATTG
CTTTCTGACTGTGTTCAGTCCTTTTATGATGGGAGCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTT
CTTGTTATGTGTGCTTTTGAAGCAGTTCAGTTGACTACTCAGTTATCGTCAAAAAGCCTTTTTCTCATTG
TTCTCGTCATATCAGACATTATGGCTTTGCATTTTTTCTTCTTGGTCAAGGATTATGGCAGCTGGCTTGA
TATTGGGACAAGCATCAGCCATTATGTGATTGTCATGTCCATGACCATCTTTTTGGTGTTCCTCAATGGC
CTGGCCCAGCTGCTCACAACGAAGAAACTCAGACTATGTGGCAAACCCAAAAGTCACTTCATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222950 representing NM_012327
Red=Cloning site Green=Tags(s)

MLLFFTLGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPARRLVLFVADGLRADALYELDENGNSRA
PFIRNIIMHEGSWGISHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPD
ILPMFAKGASGDHVYTYSYDAKREDFGAQDATKLDTWVFDNVKDFFHHARNNQSLFSKINEEKIVFFLHL
LGIDTNGHAHRPSSRDYKDNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHPSETL
TPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPLMTSLIGVPFPLNSVGILPVDYLN
NTDLFKAESMFTNAVQILEQFKVKMTQKKEVTLPFLFTPFKLLSDSKQFNILRKARSYIKHRKFDEVVSL
CKELIHLALKGLSYYHTYDRFFLGVNVVIGFVGWISYASLLIIKSHSNLIKGVSKEVKKPSHLLPCSFVA
IGILVAFFLLIQACPWTYYVYGLLPLPIWYAVLREFQVIQDLVVSVLTYPLSHFVGYLLAFTLGIEVLVL
SFFYRYMLTAGLTAFAAWPFLTRLWTRAKMTSLSWTFFSLLLAVFPLMPVVGRKPDIFLVMGAGLLVLLL
SLCVVTSLMKRKDSFIKEELLVHLLQVLSTVLSMYVVYSTQSSLLRKQGLPLMNQIISWATLASSLVVPL
LSSPVLFQRLFSILLSLMSTYLLLSTGYEALFPLVLSCLMFVWINIEQETLQQSGVCCKQKLTSIQFSYN
TDITQFRQLYLDDIRRAFFLVFFLVTAFFGTGNIASINSFDLASVYCFLTVFSPFMMGALMMWKILIPFV
LVMCAFEAVQLTTQLSSKSLFLIVLVISDIMALHFFFLVKDYGSWLDIGTSISHYVIVMSMTIFLVFLNG
LAQLLTTKKLRLCGKPKSHFM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012327
ORF Size 2793 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012327.6
RefSeq Size 4751 bp
RefSeq ORF 2796 bp
Locus ID 23556
UniProt ID O95427, A0A024R2C3
Cytogenetics 18q21.33
Domains PigN
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
MW 105.6 kDa
Summary This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor. Two alternatively spliced variants, which encode an identical isoform, have been reported. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_012327" in other vectors (4)
SKU Description Size Price
RC222950L3 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2, Myc-DDK-tagged 10 ug
$1,152.00
RC222950L4 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2, mGFP tagged 10 ug
$1,152.00
RG222950 PIGN (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2 10 ug
$789.00 $1,052.00
SC110315 PIGN (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 2 10 ug
$853.00

Plasmid Map for RC222950

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222950
Circular map for RC222950

file-alt Citations

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