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IFT46 (NM_020153) Human Tagged ORF Clone

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SKU
RC222926
IFT46 (Myc-DDK-tagged)-Human intraflagellar transport 46 homolog (Chlamydomonas) (IFT46), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IFT46
Synonyms C11orf2; C11orf60; CFAP32
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222926 representing NM_020153
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGATAACAGCAGTGATGAGTGTGAAGAGGAAAATAACAAGGTCCTAAGGGAGGGCATGCCACAGG
CTCCAGGCCACAGAGGCAAAGACATGGACCCTGTTCCACCTGCCCCTGCAAGTCTCAAGTGCCACCAGAC
CCCTTCTCATGTACTTGAGAGGGTGGGATGGTACAGAGAGAGCCAGAAGCACAGAAAGGAGAAGAAGAAG
ACCTCACAGTTGACACCTCAACGGGGCTTTAGTGAAAATGAGGATGACGATGATGATGATGATGATTCAT
CTGAAACTGATTCTGATTCTGATGATGATGATGAAGAGCATGGAGCCCCTCTGGAAGGGGCCTATGACCC
TGCAGACTATGAGCATTTGCCAGTTTCTGCTGAAATTAAGGAACTCTTCCAGTACATCAGTAGGTACACA
CCTCAGTTGATTGACCTGGACCACAAACTGAAGCCTTTCATTCCTGATTTTATCCCAGCTGTCGGGGATA
TTGATGCATTCTTAAAGGTCCCACGTCCTGATGGAAAGCCTGACAACCTTGGCCTATTGGTATTGGATGA
ACCTTCTACAAAGCAGTCAGACCCTACGGTGCTCTCACTCTGGTTAACAGAGAATTCTAAGCAGCACAAC
ATCACACAACATATGAAAGTAAAAAGCCTAGAAGATGCAGAAAAGAATCCCAAAGCCATTGACACGTGGA
TTGAGAGCATCTCTGAATTACACCGTTCTAAGCCCCCTGCGACTGTGCACTACACCAGGCCCATGCCCGA
CATTGACACGCTGATGCAGGAATGGTCCCCGGAGTTTGAAGAGCTTTTGGGCAAGGTAAGCCTGCCCACG
GCAGAGATTGATTGCAGCCTGGCAGAGTACATTGACATGATCTGTGCCATTCTAGACATCCCTGTCTACA
AGAGTCGGATCCAGTCCCTCCATCTGCTCTTTTCCCTCTACTCAGAATTCAAGAACTCACAGCATTTTAA
AGCTCTCGCTGAAGGCAAGAAAGCATTCACTCCTTCATCCAATTCCACCTCCCAAGCTGGAGACATGGAG
ACATTAACCTTCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222926 representing NM_020153
Red=Cloning site Green=Tags(s)

MADNSSDECEEENNKVLREGMPQAPGHRGKDMDPVPPAPASLKCHQTPSHVLERVGWYRESQKHRKEKKK
TSQLTPQRGFSENEDDDDDDDDSSETDSDSDDDDEEHGAPLEGAYDPADYEHLPVSAEIKELFQYISRYT
PQLIDLDHKLKPFIPDFIPAVGDIDAFLKVPRPDGKPDNLGLLVLDEPSTKQSDPTVLSLWLTENSKQHN
ITQHMKVKSLEDAEKNPKAIDTWIESISELHRSKPPATVHYTRPMPDIDTLMQEWSPEFEELLGKVSLPT
AEIDCSLAEYIDMICAILDIPVYKSRIQSLHLLFSLYSEFKNSQHFKALAEGKKAFTPSSNSTSQAGDME
TLTFS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020153
ORF Size 1065 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020153.3, NP_064538.3
RefSeq Size 1834 bp
RefSeq ORF 1068 bp
Locus ID 56912
UniProt ID Q9NQC8
Cytogenetics 11q23.3
MW 39.9 kDa
Summary Forms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. May play a role in chondrocyte maturation and skeletogenesis (By similarity).UniProtKB/Swiss-Prot Function
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"NM_020153" in other vectors (6)
SKU Description Size Price
RC222926L1 Lenti ORF clone of Human intraflagellar transport 46 homolog (Chlamydomonas) (IFT46), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC222926L2 Lenti ORF clone of Human intraflagellar transport 46 homolog (Chlamydomonas) (IFT46), transcript variant 1, mGFP tagged 10 ug
$757.00
RC222926L3 Lenti ORF clone of Human intraflagellar transport 46 homolog (Chlamydomonas) (IFT46), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC222926L4 Lenti ORF clone of Human intraflagellar transport 46 homolog (Chlamydomonas) (IFT46), transcript variant 1, mGFP tagged 10 ug
$757.00
RG222926 IFT46 (tGFP-tagged) - Human intraflagellar transport 46 homolog (Chlamydomonas) (IFT46), transcript variant 1 10 ug
$657.00
SC313108 IFT46 (untagged)-Human intraflagellar transport 46 homolog (Chlamydomonas) (IFT46), transcript variant 1 10 ug
$503.00

Plasmid Map for RC222926

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222926
Circular map for RC222926

file-alt Citations

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