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CDC42BPG (NM_017525) Human Tagged ORF Clone

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SKU
RC222875
CDC42BPG (Myc-DDK-tagged)-Human CDC42 binding protein kinase gamma (DMPK-like) (CDC42BPG)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,327.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDC42BPG
Synonyms DMPK2; HSMDPKIN; KAPPA-200; MRCKG; MRCKgamma
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222875 representing NM_017525
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGCGGCTGCGCGCGCTGGAGCAGCTGGCGCGGGGCGAGGCCGGCGGCTGCCCGGGGCTCGACG
GCCTCCTAGATCTGCTGCTGGCGCTGCACCACGAGCTCAGCAGCGGCCCCCTACGGCGGGAGCGCAGCGT
GGCGCAGTTCCTGAGCTGGGCCAGCCCCTTCGTATCAAAGGTGAAAGAACTGCGTCTGCAGAGAGATGAC
TTTGAGATCTTGAAGGTGATCGGCCGAGGAGCCTTTGGGGAGGTCACCGTGGTGAGGCAGAGGGACACTG
GGCAGATTTTTGCCATGAAAATGCTGCACAAGTGGGAGATGCTGAAGAGGGCTGAGACAGCCTGTTTCCG
GGAGGAGCGGGATGTGCTCGTGAAAGGGGACAGCCGTTGGGTGACCACTCTGCACTATGCCTTCCAAGAC
GAGGAGTACCTGTACCTTGTGATGGACTACTATGCTGGTGGGGACCTCCTGACGCTGCTGAGCCGCTTCG
AGGACCGTCTCCCGCCCGAGCTGGCCCAGTTCTACCTGGCTGAGATGGTGCTGGCCATCCACTCGCTGCA
CCAGCTGGGTTATGTCCACAGGGATGTCAAGCCAGACAACGTCCTGCTGGATGTGAACGGGCACATTCGC
CTGGCTGACTTCGGCTCCTGCCTGCGTCTCAACACCAACGGCATGGTGGATTCATCAGTGGCAGTAGGGA
CGCCGGACTATATCTCCCCTGAGATCCTGCAGGCCATGGAGGAGGGCAAGGGCCACTACGGCCCACAGTG
TGACTGGTGGTCGCTTGGAGTCTGCGCCTATGAGCTGCTCTTTGGGGAGACGCCCTTCTATGCTGAGTCC
TTGGTGGAAACCTACGGCAAGATCATGAACCACGAGGACCACCTGCAGTTCCCCCCGGACGTGCCTGACG
TGCCAGCCAGCGCCCAAGACCTGATCCGCCAGCTGCTGTGTCGCCAGGAAGAGCGGCTAGGCCGTGGTGG
GCTGGATGACTTCCGGAACCATCCTTTCTTCGAAGGCGTGGACTGGGAGCGGCTGGCGAGCAGCACGGCC
CCCTATATTCCTGAGCTGCGGGGGCCCATGGACACCTCCAACTTTGATGTGGATGACGACACCCTCAACC
ATCCAGGGACCCTGCCACCGCCCTCCCACGGGGCCTTCTCCGGCCATCACCTGCCATTCGTGGGCTTCAC
CTACACCTCAGGCAGTCACAGTCCTGAGAGCAGCTCTGAGGCTTGGGCTGCCCTGGAGCGGAAGCTCCAG
TGTCTGGAGCAGGAGAAGGTGGAGCTGAGCAGGAAGCACCAAGAGGCCCTGCACGCCCCCACAGACCATC
GGGAGCTGGAGCAGCTACGGAAGGAAGTGCAGACTCTGCGGGACAGGCTGCCAGAGATGCTGAGGGACAA
GGCCTCATTGTCCCAGACGGATGGGCCCCCAGCTGGTAGCCCAGGTCAGGACAGTGACCTACGGCAGGAG
CTTGACCGACTTCACCGGGAGCTGGCCGAGGGTCGGGCAGGGCTGCAGGCTCAGGAGCAGGAGCTCTGCA
GGGCCCAGGGGCAGCAGGAGGAGCTGCTTCAGAGGCTACAGGAGGCCCAGGAGAGAGAGGCGGCCACAGC
TAGCCAGACCCGGGCCCTGAGCTCCCAGCTGGAGGAAGCCCGGGCTGCCCAGAGGGAGCTGGAGGCCCAG
GTGTCCTCCCTGAGCCGGCAGGTGACGCAGCTGCAGGGACAGTGGGAGCAACGCCTTGAGGAGTCGTCCC
AGGCCAAGACCATCCACACAGCCTCTGAGACCAACGGGATGGGACCCCCTGAGGGTGGGCCTCAGGAGGC
CCAACTGAGGAAGGAGGTGGCCGCCCTGCGAGAGCAGCTGGAGCAGGCCCACAGCCACAGGCCGAGTGGT
AAGGAGGAGGCTCTGTGCCAGCTGCAGGAGGAAAACCGGAGGCTGAGCCGGGAGCAGGAGCGGCTAGAAG
CAGAGCTGGCCCAGGAGCAGGAGAGCAAGCAGCGGCTGGAGGGTGAGCGGCGGGAGACGGAGAGCAACTG
GGAGGCCCAGCTCGCCGACATCCTCAGCTGGGTGAATGATGAGAAGGTCTCAAGAGGCTACCTGCAGGCC
CTGGCCACCAAGATGGCAGAGGAGCTGGAGTCCTTGAGGAACGTAGGCACCCAGACGCTCCCTGCCCGGC
CACTGGACCACCAGTGGAAGGCGCGGCGACTGCAGAAGATGGAGGCCTCGGCCAGGCTGGAGCTGCAGTC
AGCGCTGGAGGCCGAGATCCGCGCCAAGCAGGGCCTGCAGGAGCGGCTGACACAGGTGCAGGAGGCCCAG
CTGCAGGCTGAGCGCCGTCTGCAGGAGGCCGAGAAGCAGAGCCAGGCCCTGCAACAGGAGCTCGCCATGC
TGCGGGAGGAGCTGCGGGCCCGAGGGCCAGTGGACACCAAGCCCTCAAACTCCCTGATTCCCTTCCTGTC
CTTCCGGAGCTCAGAGAAGGATTCTGCCAAGGACCCTGGCATCTCAGGAGAGGCCACAAGGCATGGAGGA
GAGCCAGATCTGAGGCCGGAGGGCCGACGCAGCCTGCGCATGGGGGCTGTGTTCCCCAGAGCACCCACTG
CCAACACAGCCTCTACAGAAGGTCTTCCTGCTAAGCCCGGCTCACACACGCTGCGCCCCCGGAGCTTCCC
ATCCCCGACCAAGTGTCTCCGCTGCACCTCGCTGATGCTGGGCCTGGGCCGCCAGGGCCTGGGTTGTGAT
GCCTGCGGCTACTTTTGTCACACAACCTGTGCCCCACAGGCCCCACCCTGCCCCGTGCCCCCTGACCTCC
TCCGCACAGCCCTGGGAGTACACCCCGAAACAGGCACAGGCACTGCCTATGAGGGCTTTCTGTCGGTGCC
GCGGCCCTCAGGTGTCCGGCGGGGCTGGCAGCGCGTGTTTGCTGCCCTGAGTGACTCACGCCTGCTGCTG
TTTGACGCCCCTGACCTGAGGCTCAGCCCGCCCAGTGGGGCCCTCCTGCAGGTCCTAGATCTGAGGGACC
CCCAGTTCTCGGCTACCCCTGTCCTGGCCTCTGATGTTATCCATGCCCAATCCAGGGACCTGCCACGCAT
CTTTAGGGTGACAACCTCCCAGCTGGCAGTGCCGCCCACCACGTGCACTGTGCTGCTGCTGGCAGAGAGC
GAGGGGGAGCGGGAACGCTGGCTGCAGGTGCTGGGTGAGCTGCAGCGGCTGCTGCTGGACGCGCGGCCAA
GACCCCGGCCCGTGTACACACTCAAGGAGGCTTACGACAACGGGCTGCCGCTGCTGCCTCACACGCTCTG
CGCTGCCATCCTCGACCAGGATCGACTTGCGCTTGGCACCGAGGAGGGGCTCTTTGTCATCCATCTGCGC
AGCAACGACATCTTCCAGGTGGGGGAGTGCCGGCGCGTGCAGCAGCTGACCTTGAGCCCCAGTGCAGGCC
TGCTGGTCGTGCTGTGTGGCCGCGGCCCCAGCGTGCGTCTCTTTGCCCTGGCGGAGCTGGAGAACATAGA
GGTAGCAGGTGCCAAGATCCCCGAGTCTCGAGGCTGCCAGGTGCTGGCAGCTGGAAGCATCCTGCAGGCC
CGCACCCCGGTGCTCTGTGTAGCCGTCAAGCGCCAGGTGCTCTGCTACCAGCTGGGCCCGGGCCCTGGGC
CCTGGCAGCGCCGCATCCGTGAGCTGCAGGCACCTGCCACTGTGCAGAGCCTGGGGCTGCTGGGCGACCG
GCTATGTGTGGGCGCCGCCGGTGGCTTTGCACTCTACCCGCTGCTCAACGAGGCTGCGCCGTTGGCGCTG
GGGGCCGGTTTGGTGCCTGAGGAGCTGCCACCATCCCGCGGGGGCCTGGGTGAGGCACTGGGTGCCGTGG
AGCTTAGCCTCAGCGAGTTCCTGCTACTCTTCACCACTGCTGGCATCTACGTGGATGGCGCAGGCCGCAA
GTCTCGTGGCCACGAGCTGTTGTGGCCAGCAGCGCCCATGGGCTGGGGGTATGCGGCCCCCTACCTGACA
GTGTTCAGCGAGAACTCCATCGATGTGTTTGACGTGAGGAGGGCAGAATGGGTGCAGACCGTGCCGCTCA
AGAAGGTGCGGCCCCTCAATCCAGAGGGCTCCCTGTTCCTCTACGGCACCGAGAAGGTCCGCCTGACCTA
CCTCAGGAACCAGCTGGCAGAGAAGGACGAGTTCGACATCCCGGACCTCACCGACAACAGCCGGCGCCAG
CTGTTCCGCACCAAGAGCAAGCGCCGCTTCTTTTTCCGCGTGTCGGAGGAGCAGCAGAAGCAGCAGCGCA
GGGAGATGCTGAAGGACCCTTTTGTGCGCTCCAAGCTCATCTCGCCGCCTACCAACTTCAACCACCTAGT
ACACGTGGGCCCTGCCAACGGGCGGCCCGGCGCCAGGGACAAGTCCCCGGCTCCCGAAGAGAAGGGCCGA
GTTGCCCGCGGCTCCGGCCCACAGCGGCCCCACAGCTTCTCCGAGGCGTTGCGGCGCCCAGCCTCCATGG
GCAGCGAAGGCCTCGGTGGAGACGCAGACCCCATGAAGAGGAAACCCTGGACATCCCTGTCCAGCGAGTC
TGTGTCCTGCCCCCAGGGATCGCTGAGCCCTGCAACCTCCCTAATGCAGGTCTCAGAACGGCCCCGAAGC
CTCCCCCTATCCCCTGAATTGGAGAGCTCTCCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222875 protein sequence
Red=Cloning site Green=Tags(s)

MERRLRALEQLARGEAGGCPGLDGLLDLLLALHHELSSGPLRRERSVAQFLSWASPFVSKVKELRLQRDD
FEILKVIGRGAFGEVTVVRQRDTGQIFAMKMLHKWEMLKRAETACFREERDVLVKGDSRWVTTLHYAFQD
EEYLYLVMDYYAGGDLLTLLSRFEDRLPPELAQFYLAEMVLAIHSLHQLGYVHRDVKPDNVLLDVNGHIR
LADFGSCLRLNTNGMVDSSVAVGTPDYISPEILQAMEEGKGHYGPQCDWWSLGVCAYELLFGETPFYAES
LVETYGKIMNHEDHLQFPPDVPDVPASAQDLIRQLLCRQEERLGRGGLDDFRNHPFFEGVDWERLASSTA
PYIPELRGPMDTSNFDVDDDTLNHPGTLPPPSHGAFSGHHLPFVGFTYTSGSHSPESSSEAWAALERKLQ
CLEQEKVELSRKHQEALHAPTDHRELEQLRKEVQTLRDRLPEMLRDKASLSQTDGPPAGSPGQDSDLRQE
LDRLHRELAEGRAGLQAQEQELCRAQGQQEELLQRLQEAQEREAATASQTRALSSQLEEARAAQRELEAQ
VSSLSRQVTQLQGQWEQRLEESSQAKTIHTASETNGMGPPEGGPQEAQLRKEVAALREQLEQAHSHRPSG
KEEALCQLQEENRRLSREQERLEAELAQEQESKQRLEGERRETESNWEAQLADILSWVNDEKVSRGYLQA
LATKMAEELESLRNVGTQTLPARPLDHQWKARRLQKMEASARLELQSALEAEIRAKQGLQERLTQVQEAQ
LQAERRLQEAEKQSQALQQELAMLREELRARGPVDTKPSNSLIPFLSFRSSEKDSAKDPGISGEATRHGG
EPDLRPEGRRSLRMGAVFPRAPTANTASTEGLPAKPGSHTLRPRSFPSPTKCLRCTSLMLGLGRQGLGCD
ACGYFCHTTCAPQAPPCPVPPDLLRTALGVHPETGTGTAYEGFLSVPRPSGVRRGWQRVFAALSDSRLLL
FDAPDLRLSPPSGALLQVLDLRDPQFSATPVLASDVIHAQSRDLPRIFRVTTSQLAVPPTTCTVLLLAES
EGERERWLQVLGELQRLLLDARPRPRPVYTLKEAYDNGLPLLPHTLCAAILDQDRLALGTEEGLFVIHLR
SNDIFQVGECRRVQQLTLSPSAGLLVVLCGRGPSVRLFALAELENIEVAGAKIPESRGCQVLAAGSILQA
RTPVLCVAVKRQVLCYQLGPGPGPWQRRIRELQAPATVQSLGLLGDRLCVGAAGGFALYPLLNEAAPLAL
GAGLVPEELPPSRGGLGEALGAVELSLSEFLLLFTTAGIYVDGAGRKSRGHELLWPAAPMGWGYAAPYLT
VFSENSIDVFDVRRAEWVQTVPLKKVRPLNPEGSLFLYGTEKVRLTYLRNQLAEKDEFDIPDLTDNSRRQ
LFRTKSKRRFFFRVSEEQQKQQRREMLKDPFVRSKLISPPTNFNHLVHVGPANGRPGARDKSPAPEEKGR
VARGSGPQRPHSFSEALRRPASMGSEGLGGDADPMKRKPWTSLSSESVSCPQGSLSPATSLMQVSERPRS
LPLSPELESSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_017525
ORF Size 4653 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017525.3
RefSeq Size 4680 bp
RefSeq ORF 4656 bp
Locus ID 55561
UniProt ID Q6DT37
Cytogenetics 11q13.1
Protein Families Druggable Genome, Protein Kinase
MW 172.3 kDa
Summary May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_017525" in other vectors (6)
SKU Description Size Price
RC222875L1 Lenti ORF clone of Human CDC42 binding protein kinase gamma (DMPK-like) (CDC42BPG), Myc-DDK-tagged 10 ug
$1,627.00
RC222875L2 Lenti ORF clone of Human CDC42 binding protein kinase gamma (DMPK-like) (CDC42BPG), mGFP tagged 10 ug
$1,627.00
RC222875L3 Lenti ORF clone of Human CDC42 binding protein kinase gamma (DMPK-like) (CDC42BPG), Myc-DDK-tagged 10 ug
$1,627.00
RC222875L4 Lenti ORF clone of Human CDC42 binding protein kinase gamma (DMPK-like) (CDC42BPG), mGFP tagged 10 ug
$1,627.00
RG222875 CDC42BPG (tGFP-tagged) - Human CDC42 binding protein kinase gamma (DMPK-like) (CDC42BPG) 10 ug
$1,527.00
SC304473 CDC42BPG (untagged)-Human CDC42 binding protein kinase gamma (DMPK-like) (CDC42BPG) 10 ug
$1,460.00

file-alt Citations

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