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MPIG6B (NM_138277) Human Tagged ORF Clone

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SKU
RC222783
C6orf25 (Myc-DDK-tagged)-Human chromosome 6 open reading frame 25 (C6orf25), transcript variant 7
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MPIG6B
Synonyms C6orf25; G6b; G6b-B; NG31; THAMY
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222783 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTGTTTCTGCAGCTGCTACCGCTGCTGCTCTCGAGGGCCCAAGGGAACCCTGGGGCTTCTCTGG
ACGGCCGCCCTGGGGACCGGGTGAATCTCTCCTGCGGAGGAGTCTCTCATCCCATCCGCTGGGTCTGGGC
ACCCAGCTTCCCGGCCTGCAAGGGCCTGTCCAAAGGACGCCGACCGATCCTGTGGGCCTCTTCGAGCGGG
ACCCCCACCGTGCCTCCCCTCCAGCCTTTCGTCGGCCGCCTACGCTCCCTGGACTCTGGTATCCGGCGGC
TGGAGCTCCTCTTGAGCGCGGGGGACTCGGGCACTTTTTTCTGCAAGGGCCGCCACGAGGACGAGAGCCG
TACAGTGCTTCACGTGCTGGGGGACAGGACCTATTGCAAGGCCCCCGGGCCTACCCATGGGTCCGTGTAT
CCCCAGCTCCTGATCCCGCTGCTGGGCGCTGGGTTGGTGCTCGGACTGGGAGCTTTGGGCCTGGTCTGGT
GGCTGCACAGGCGCCTGCCCCCGCAACCGATTCGACCACTCCCTAGATTTGCTCTGTCCCCCCCACATAG
CTCCACTTGTGAAAACCGAGCCCCAGAGGCCAGTAAAGGAGGAAGAGCCCAAGATTCCAGGGGACCTGGA
CCAGGAACCGGTAAGGGCATGGGGATGGGAAGGGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222783 protein sequence
Red=Cloning site Green=Tags(s)

MAVFLQLLPLLLSRAQGNPGASLDGRPGDRVNLSCGGVSHPIRWVWAPSFPACKGLSKGRRPILWASSSG
TPTVPPLQPFVGRLRSLDSGIRRLELLLSAGDSGTFFCKGRHEDESRTVLHVLGDRTYCKAPGPTHGSVY
PQLLIPLLGAGLVLGLGALGLVWWLHRRLPPQPIRPLPRFALSPPHSSTCENRAPEASKGGRAQDSRGPG
PGTGKGMGMGRG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138277
ORF Size 666 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138277.2, NP_612121.1
RefSeq Size 2560 bp
RefSeq ORF 669 bp
Locus ID 80739
UniProt ID O95866, B0V023
Cytogenetics 6p21.33
Protein Families Druggable Genome, Transmembrane
MW 23.6 kDa
Summary This gene is a member of the immunoglobulin (Ig) superfamily and is located in the major histocompatibility complex (MHC) class III region. The protein encoded by this gene is a glycosylated, plasma membrane-bound cell surface receptor, but soluble isoforms encoded by some transcript variants have been found in the endoplasmic reticulum and Golgi before being secreted. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
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"NM_138277" in other vectors (6)
SKU Description Size Price
RC222783L1 Lenti ORF clone of Human chromosome 6 open reading frame 25 (C6orf25), transcript variant 7, Myc-DDK-tagged 10 ug
$600.00
RC222783L2 Lenti ORF clone of Human chromosome 6 open reading frame 25 (C6orf25), transcript variant 7, mGFP tagged 10 ug
$600.00
RC222783L3 Lenti ORF clone of Human chromosome 6 open reading frame 25 (C6orf25), transcript variant 7, Myc-DDK-tagged 10 ug
$600.00
RC222783L4 Lenti ORF clone of Human chromosome 6 open reading frame 25 (C6orf25), transcript variant 7, mGFP tagged 10 ug
$600.00
RG222783 C6orf25 (tGFP-tagged) - Human chromosome 6 open reading frame 25 (C6orf25), transcript variant 7 10 ug
$500.00
SC305996 C6orf25 (untagged)-Human chromosome 6 open reading frame 25 (C6orf25), transcript variant 7 10 ug
$330.00

Plasmid Map for RC222783

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222783
Circular map for RC222783

file-alt Citations

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