PON2 (NM_001018161) Human Tagged ORF Clone

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SKU
RC222678
PON2 (Myc-DDK-tagged)-Human paraoxonase 2 (PON2), transcript variant 2
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PON2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222678 representing NM_001018161
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCGGCTGGTGGCTGTGGGCTTGCTGGGGATCGCGCTGGCGCTCCTGGGCGAGAGGCTTCTGGCAC
TCAGAAATCGACTTAAAGCCTCCAGAGAAGTAGAATCTGTAGACCTTCCACACTGCCACCTGATTAAAGG
AATTGAAGCTGGCTCTGAAGATATTGACATACTTCCCAATGGTCTGGCTTTTTTTAGTGTGGGTCTAAAA
TTCCCAGGACTCCACAGCTTTGCACCAGATAAGCCTGGAGGAATACTAATGATGGATCTAAAAGAAGAAA
AACCAAGGGCACGGGAATTAAGAATCAGTCGTGGGTTTGATTTGGCCTCATTCAATCCACATGGCATCAG
CACTTTCATAGACAACGAATTCAAGAATACAGTGGAAATTTTTAAATTTGAAGAAGCAGAAAATTCTCTG
TTGCATCTGAAAACAGTCAAACATGAGCTTCTTCCAAGTGTGAATGACATCACAGCTGTTGGACCGGCAC
ATTTCTATGCCACAAATGACCACTACTTCTCTGATCCTTTCTTAAAGTATTTAGAAACATACTTGAACTT
ACACTGGGCAAATGTTGTTTACTACAGTCCAAATGAAGTTAAAGTGGTAGCAGAAGGATTTGATTCAGCA
AATGGGATCAATATTTCACCTGATGATAAGTATATCTATGTTGCTGACATATTGGCTCATGAAATTCATG
TTTTGGAAAAACACACTAATATGAATTTAACTCAGTTGAAGGTACTTGAGCTGGATACACTGGTGGATAA
TTTATCTATTGATCCTTCCTCGGGGGACATCTGGGTAGGCTGTCATCCTAATGGCCAGAAGCTCTTCGTG
TATGACCCGAACAATCCTCCCTCGTCAGAGGTTCTCCGCATCCAGAACATTCTATCTGAGAAGCCTACAG
TGACTACAGTTTATGCCAACAATGGGTCTGTTCTCCAAGGAAGTTCTGTAGCCTCAGTGTATGATGGGAA
GCTGCTCATAGGCACTTTATACCACAGAGCCTTGTATTGTGAACTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC222678 representing NM_001018161
Red=Cloning site Green=Tags(s)

MGRLVAVGLLGIALALLGERLLALRNRLKASREVESVDLPHCHLIKGIEAGSEDIDILPNGLAFFSVGLK
FPGLHSFAPDKPGGILMMDLKEEKPRARELRISRGFDLASFNPHGISTFIDNEFKNTVEIFKFEEAENSL
LHLKTVKHELLPSVNDITAVGPAHFYATNDHYFSDPFLKYLETYLNLHWANVVYYSPNEVKVVAEGFDSA
NGINISPDDKYIYVADILAHEIHVLEKHTNMNLTQLKVLELDTLVDNLSIDPSSGDIWVGCHPNGQKLFV
YDPNNPPSSEVLRIQNILSEKPTVTTVYANNGSVLQGSSVASVYDGKLLIGTLYHRALYCEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001018161
ORF Size 1026 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001018161.1, NP_001018171.1
RefSeq Size 1633 bp
RefSeq ORF 1029 bp
Locus ID 5445
UniProt ID Q15165
Cytogenetics 7q21.3
Protein Families Druggable Genome
Protein Pathways Metabolic pathways
MW 37.8 kDa
Summary This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001018161" in other vectors (4)
SKU Description Size Price
RC222678L3 Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 2, Myc-DDK-tagged 10 ug
$986.00
RC222678L4 Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 2, mGFP tagged 10 ug
$986.00
RG222678 PON2 (tGFP-tagged) - Human paraoxonase 2 (PON2), transcript variant 2 10 ug
$886.00
SC302202 PON2 (untagged)-Human paraoxonase 2 (PON2), transcript variant 2 10 ug
$686.00

Plasmid Map for RC222678

Download sequence for

Cloning scheme for RC222678

Circular map for RC222678

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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