Proprotein Convertase 2 (PCSK2) (NM_002594) Human Tagged ORF Clone
SKU
RC222498
PCSK2 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1
$594.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Proprotein Convertase 2 |
| Synonyms | NEC-2; NEC 2; NEC2; PC2; SPC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002594 |
| ORF Size | 1914 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002594.5 |
| RefSeq Size | 4745 bp |
| RefSeq ORF | 1917 bp |
| Locus ID | 5126 |
| UniProt ID | P16519 |
| Cytogenetics | 20p12.1 |
| Domains | P, Peptidase_S8 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 70.57 kDa |
| Summary | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The protein undergoes an initial autocatalytic processing event and interacts with a neuroendocrine secretory protein in the ER, exits the ER and sorts to secretory granules, where it is cleaved and catalytically activated during intracellular transport. The encoded protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Jan 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002594" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC222498L1 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$894.00
|
|
| RC222498L2 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, mGFP tagged | 10 ug |
$894.00
|
|
| RC222498L3 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$894.00
|
|
| RC222498L4 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, mGFP tagged | 10 ug |
$894.00
|
|
| RG222498 | PCSK2 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1 | 10 ug |
$489.00
$794.00
|
|
| SC118531 | PCSK2 (untagged)-Human proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1 | 10 ug |
$595.00
|
|
Citations
The nutrient and energy sensor Sirt1 regulates the hypothalamic-pituitary-adrenal (HPA) axis by altering the production of the prohormone convertase 2 (PC2) essential in the maturation of corticotropin releasing hormone (CRH) from its prohormone in male rats
Toorie, Cyr, Steger et al
J. Biol. Chem. (2016)
Toorie, Cyr, Steger et al
J. Biol. Chem. (2016)
AI Summary: OriGene products contributed to this study by providing CMV plasmids containing PC2 cDNA and preproCRH cDNA, which were used for transient co-transfection to measure intracellular and released CRH content.
Snippet: μg/ml streptomycin. To measure intracellular and released CRH content, cells were transiently co-transfected with a CMV plasmid containing PC2 cDNA and a CMV- plasmid containing preproCRH cDNA (Origene). For Sirt1 ... Cite SaveSaving
Showing 1-1 of 1 papers.
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