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BAX (NM_138763) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC222409L3
Lenti ORF clone of Human BCL2-associated X protein (BAX), transcript variant delta, Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $450.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BAX
Synonyms BCL2L4
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC222409).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138763
ORF Size 429 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138763.2
RefSeq Size 741 bp
RefSeq ORF 432 bp
Locus ID 581
UniProt ID Q07812
Cytogenetics 19q13.33
Protein Families Druggable Genome, Transmembrane
Protein Pathways Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Huntington's disease, Neurotrophin signaling pathway, p53 signaling pathway, Pathways in cancer, Prion diseases
MW 15.6 kDa
Summary The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. provided by RefSeq, Dec 2019
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Documents
Resources
"NM_138763" in other vectors (6)
SKU Description Size Price
RC222409 BAX (Myc-DDK-tagged)-Human BCL2-associated X protein (BAX), transcript variant delta 10 ug
$150.00
RC222409L1 Lenti ORF clone of Human BCL2-associated X protein (BAX), transcript variant delta, Myc-DDK-tagged 10 ug
$450.00
RC222409L2 Lenti ORF clone of Human BCL2-associated X protein (BAX), transcript variant delta, mGFP tagged 10 ug
$450.00
RC222409L4 Lenti ORF clone of Human BCL2-associated X protein (BAX), transcript variant delta, mGFP tagged 10 ug
$450.00
RG222409 BAX (tGFP-tagged) - Human BCL2-associated X protein (BAX), transcript variant delta 10 ug
$489.00
SC306076 BAX (untagged)-Human BCL2-associated X protein (BAX), transcript variant delta 10 ug
$150.00

Plasmid Map for RC222409L3

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Cloning scheme for RC222409L3
Circular map for RC222409L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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