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MTMR7 (NM_004686) Human Tagged ORF Clone

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SKU
RC222373
MTMR7 (Myc-DDK-tagged)-Human myotubularin related protein 7 (MTMR7)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $614.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MTMR7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222373 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCACATCCGCACGCCCAAGGTTGAAAATGTCCGCTTGGTAGATCGAGTGTCTCCTAAAAAAGCAG
CTCTAGGTACTTTGTATTTGACGGCTACCCATGTCATATTCGTGGAAAATTCACCTGACGCAAGAAAAGA
AACATGGATTCTTCACAGTCAGATTTCCACCATTGAGAAACAGGCAACAACCGCTACCGGATGCCCTCTG
CTGATTCGCTGCAAGAACTTTCAGATAATACAGCTCATCATACCTCAGGAAAGAGATTGCCACGACGTGT
ACATCTCCCTGATACGCCTTGCAAGGCCAGTGAAATATGAGGAGTTATACTGCTTTTCATTCAACCCCAT
GCTGGATAAAGAAGAAAGAGAGCAAGGCTGGGTGCTGATCGATCTTAGTGAAGAATACACGCGGATGGGC
CTCCCTAATCATTACTGGCAGCTCAGCGATGTGAATAGAGACTATAGAGTCTGTGACTCTTATCCTACTG
AACTGTACGTTCCCAAATCGGCCACGGCACACATCATAGTGGGGAGTTCCAAATTCCGGAGTAGACGGCG
ATTTCCTGTCCTTTCTTACTATTATAAAGATAACCACGCCTCCATCTGCCGGAGCAGCCAGCCCCTGTCC
GGCTTCAGTGCCCGGTGCCTAGAGGACGAGCAGATGCTCCAGGCCATTAGGAAAGCCAATCCAGGAAGTG
ACTTCGTTTATGTCGTTGACACCCGGCCTAAACTTAATGCAATGGCAAATCGTGCTGCAGGGAAAGGCTA
TGAGAATGAAGACAATTATTCCAATATCAAGTTTCAGTTTATCGGGATAGAGAACATCCATGTCATGAGG
AACAGTCTGCAGAAAATGCTGGAAGTGTGTGAACTTAAATCTCCCTCCATGAGTGATTTCCTGTGGGGTC
TGGAGAACTCTGGCTGGTTAAGGCACATTAAAGCCATAATGGATGCAGGAATCTTCATTGCAAAGGCAGT
GTCAGAGGAAGGGGCAAGTGTGCTTGTTCACTGTTCTGATGGCTGGGACAGGACCGCTCAGGTGTGCTCG
GTGGCAAGCCTGCTGCTGGACCCTCACTACCGGACTCTGAAGGGCTTCATGGTATTAATTGAAAAGGACT
GGATTTCCTTTGGTCATAAGTTTAATCACCGATATGGCAATCTAGATGGTGACCCAAAAGAAATCTCTCC
AGTTATTGACCAGTTCATTGAGTGTGTTTGGCAGTTAATGGAACAATTTCCCTGTGCCTTTGAGTTCAAT
GAGAGGTTTTTGATTCACATTCAACATCACATTTATTCCTGCCAGTTTGGAAACTTCCTATGTAACAGCC
AAAAGGAGAGACGAGAACTCAAGATTCAAGAAAGAACATACTCATTATGGGCTCACCTGTGGAAGAATCG
GGCCGACTACCTGAATCCTCTGTTTAGAGCTGATCACAGCCAGACTCAGGGAACCCTTCATCTCCCTACA
ACACCATGTAACTTCATGTACAAGTTTTGGAGTGGAATGTATAACCGCTTTGAAAAGGGGATGCAGCCCC
GACAGTCAGTTACAGATTACCTAATGGCAGTGAAGGAAGAAACTCAGCAGCTAGAGGAAGAACTAGAGGC
CCTGGAAGAAAGGCTGGAAAAAATTCAAAAGGTCCAGTTAAATTGCACTAAGGTGAAGAGTAAGCAAAGT
GAGCCCAGCAAGCACTCAGGGTTTTCTACCTCAGACAACAGCATAGCCAACACTCCCCAGGATTACAGTG
GGAATATGAAATCATTTCCATCCCGGAGCCCTTCACAAGGCGATGAAGATTCTGCTCTGATTCTAACCCA
AGACAATCTGAAAAGTTCAGATCCAGATCTGTCAGCCAACAGTGACCAAGAGTCCGGGGTGGAGGATTTG
AGCTGTCGGTCTCCAAGTGGTGGTGAGCATGCACCGAGTGAAGATAGTGGCAAGGACCGGGATTCTGATG
AAGCCGTGTTTCTCACTGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222373 protein sequence
Red=Cloning site Green=Tags(s)

MEHIRTPKVENVRLVDRVSPKKAALGTLYLTATHVIFVENSPDARKETWILHSQISTIEKQATTATGCPL
LIRCKNFQIIQLIIPQERDCHDVYISLIRLARPVKYEELYCFSFNPMLDKEEREQGWVLIDLSEEYTRMG
LPNHYWQLSDVNRDYRVCDSYPTELYVPKSATAHIIVGSSKFRSRRRFPVLSYYYKDNHASICRSSQPLS
GFSARCLEDEQMLQAIRKANPGSDFVYVVDTRPKLNAMANRAAGKGYENEDNYSNIKFQFIGIENIHVMR
NSLQKMLEVCELKSPSMSDFLWGLENSGWLRHIKAIMDAGIFIAKAVSEEGASVLVHCSDGWDRTAQVCS
VASLLLDPHYRTLKGFMVLIEKDWISFGHKFNHRYGNLDGDPKEISPVIDQFIECVWQLMEQFPCAFEFN
ERFLIHIQHHIYSCQFGNFLCNSQKERRELKIQERTYSLWAHLWKNRADYLNPLFRADHSQTQGTLHLPT
TPCNFMYKFWSGMYNRFEKGMQPRQSVTDYLMAVKEETQQLEEELEALEERLEKIQKVQLNCTKVKSKQS
EPSKHSGFSTSDNSIANTPQDYSGNMKSFPSRSPSQGDEDSALILTQDNLKSSDPDLSANSDQESGVEDL
SCRSPSGGEHAPSEDSGKDRDSDEAVFLTA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004686
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004686.5
RefSeq Size 5302 bp
RefSeq ORF 1983 bp
Locus ID 9108
UniProt ID Q9Y216, B7ZAG8, B7Z9Q7
Cytogenetics 8p22
Protein Families Phosphatase
Protein Pathways Fructose and mannose metabolism, Metabolic pathways, Riboflavin metabolism, Thiamine metabolism
MW 75.8 kDa
Summary This gene encodes a member of the myotubularin family of tyrosine/dual-specificity phosphatases. The encoded protein is characterized by four distinct domains that are conserved among all members of the myotubularin family: the glucosyltransferase, Rab-like GTPase activator and myotubularins domain, the Rac-induced recruitment domain, the protein tyrosine phosphatases and dual-specificity phosphatases domain and the suppressor of variegation 3-9, enhancer-of-zeste, and trithorax interaction domain. This protein dephosphorylates the target substrates phosphatidylinositol 3-phosphate and inositol 1,3-bisphosphate. A pseudogene of this gene is found on chromosome 5. provided by RefSeq, Mar 2009
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"NM_004686" in other vectors (6)
SKU Description Size Price
RC222373L1 Lenti ORF clone of Human myotubularin related protein 7 (MTMR7), Myc-DDK-tagged 10 ug
$914.00
RC222373L2 Lenti ORF clone of Human myotubularin related protein 7 (MTMR7), mGFP tagged 10 ug
$914.00
RC222373L3 Lenti ORF clone of Human myotubularin related protein 7 (MTMR7), Myc-DDK-tagged 10 ug
$914.00
RC222373L4 Lenti ORF clone of Human myotubularin related protein 7 (MTMR7), mGFP tagged 10 ug
$914.00
RG222373 MTMR7 (tGFP-tagged) - Human myotubularin related protein 7 (MTMR7) 10 ug
$489.00 $814.00
SC128036 MTMR7 (untagged)-Human myotubularin related protein 7 (MTMR7) 10 ug
$615.00

Plasmid Map for RC222373

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222373
Circular map for RC222373

file-alt Citations

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