ATP10B (NM_025153) Human Tagged Lenti ORF Clone
- LentiORF®
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ATP10B |
| Synonyms | ATPVB |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_025153 |
| ORF Size | 4383 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_025153.2 |
| RefSeq Size | 7582 bp |
| RefSeq ORF | 4386 bp |
| Locus ID | 23120 |
| UniProt ID | O94823 |
| Cytogenetics | 5q34 |
| Protein Families | Transmembrane |
| MW | 165.2 kDa |
| Summary | Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (Probable).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_025153" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC222292 | ATP10B (Myc-DDK-tagged)-Human ATPase, class V, type 10B (ATP10B) | 10 ug |
$589.00
$1,250.00
|
|
| RC222292L4 | Lenti ORF clone of Human ATPase, class V, type 10B (ATP10B), mGFP tagged | 10 ug |
$1,550.00
|
|
| RG222292 | ATP10B (tGFP-tagged) - Human ATPase, class V, type 10B (ATP10B) | 10 ug |
$1,450.00
|
|
| SC318625 | ATP10B (untagged)-Human ATPase, class V, type 10B (ATP10B) | 10 ug |
$1,376.00
|
|
Citations
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