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ATP10B (NM_025153) Human Untagged Clone

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SKU
SC318625
ATP10B (untagged)-Human ATPase, class V, type 10B (ATP10B)
  $1,376.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ATP10B
Synonyms ATPVB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC318625 representing NM_025153.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCCCTCTCAGTGGACTCATCGTGGCATCGGTGGCAGTGGAGAGTCAGAGATGGCTTCCCCCATTGT
CCATCGGAAACCACACCGCTGCTCTCTCCAGAGAAAGGGAGACAGAGCTACAACTTGACACAGCAGCGG
GTCGTGTTCCCCAACAACAGCATATTCCATCAAGATTGGGAAGAGGTCTCCAGGAGATACCCTGGCAAC
AGAACCTGCACAACCAAATACACCCTCTTCACCTTCCTGCCCCGGAATCTCTTTGAGCAATTTCATAGA
TGGGCTAACCTCTATTTCCTGTTCCTGGTGATTTTGAACTGGATGCCCTCCATGGAAGTCTTCCACAGA
GAAATCACCATGTTACCATTGGCCATTGTCCTGTTCGTCATCATGATCAAGGATGGCATGGAGGACTTC
AAGAGACACCGCTTTGATAAAGCAATAAACTGCTCCAACATTCGAATTTATGAAAGAAAAGAGCAGACC
TATGTGCAGAAGTGCTGGAAGGATGTGCGCGTGGGAGACTTCATCCAAATGAAATGCAATGAGATTGTC
CCAGCAGACATACTCCTCCTTTTTTCCTCTGACCCCAATGGGATATGCCATCTGGAAACTGCCAGCTTG
GATGGAGAGACAAACCTCAAGCAAAGATGTGTCGTGAAGGGCTTCTCACAGCAGGAGGTACAGTTCGAA
CCAGAGCTTTTCCACAATACCATCGTGTGTGAGAAACCCAACAACCACCTCAACAAATTTAAGGGTTAT
ATGGAGCATCCTGACCAGACCAGGACTGGCTTTGGCTGTGAGAGTCTTCTGCTTCGAGGCTGCACCATC
AGAAACACCGAGATGGCTGTTGGCATTGTCATCTATGCAGGCCATGAGACGAAAGCCATGCTGAACAAC
AGTGGCCCCCGGTACAAACGCAGCAAGATTGAGCGGCGCATGAATATAGACATCTTCTTCTGCATTGGG
ATCCTCATCCTCATGTGCCTTATTGGAGCTGTAGGTCACAGCATCTGGAATGGGACCTTTGAAGAACAC
CCTCCCTTCGATGTGCCAGATGCCAATGGCAGCTTCCTTCCCAGTGCCCTTGGGGGCTTCTACATGTTC
CTCACAATGATCATCCTGCTCCAGGTGCTGATCCCCATCTCTTTGTATGTCTCCATTGAGCTGGTGAAG
CTCGGGCAAGTGTTCTTCTTGAGCAATGACCTTGACCTGTATGATGAAGAGACCGATTTATCCATTCAA
TGTCGAGCCCTCAACATCGCAGAGGACTTGGGCCAGATCCAGTACATCTTCTCCGATAAGACGGGGACC
CTGACAGAGAACAAGATGGTGTTCCGACGTTGCACCATCATGGGCAGCGAGTATTCTCACCAAGAAAAT
GCTAAGCGACTGGAGACCCCAAAGGAGCTGGACTCAGATGGTGAAGAGTGGACCCAATACCAATGCCTG
TCCTTCTCGGCTAGATGGGCCCAGGATCCAGCAACTATGAGAAGCCAAAAAGGTGCTCAGCCTCTGAGG
AGGAGCCAGAGTGCCCGGGTGCCCATCCAGGGCCACTACCGGCAAAGGTCTATGGGGCACCGTGAAAGC
TCACAGCCTCCTGTGGCCTTCAGCAGCTCCATAGAAAAAGATGTAACTCCAGATAAAAACCTACTGACC
AAGGTTCGAGATGCTGCCCTGTGGTTGGAGACCTTGTCAGACAGCAGACCTGCCAAGGCTTCCCTCTCC
ACCACCTCCTCCATTGCTGATTTCTTCCTTGCCTTAACCATCTGCAACTCTGTCATGGTGTCCACAACC
ACCGAGCCCAGGCAGAGGGTCACCATCAAACCCTCAAGCAAGGCTCTGGGGACGTCCCTGGAGAAGATT
CAGCAGCTCTTCCAGAAGTTGAAGCTATTGAGCCTCAGCCAGTCATTCTCATCCACTGCACCCTCTGAC
ACAGACCTCGGGGAGAGCTTAGGGGCCAACGTGGCCACCACAGACTCGGATGAGAGAGATGATGCATCT
GTGTGCAGTGGAGGTGACTCCACTGATGACGGTGGCTACAGGAGCAGCATGTGGGACCAGGGCGACATC
CTGGAGTCTGGGTCAGGCACTTCCTTGGAGGAGGCATTGGAGGCCCCAGCCACAGACCTGGCCAGGCCT
GAGTTCTGTTACGAGGCTGAGAGCCCTGATGAGGCCGCCCTGGTGCACGCTGCCCATGCCTACAGCTTC
ACACTAGTGTCCCGGACACCTGAGCAGGTGACTGTGCGCCTGCCCCAGGGCACCTGCCTCACCTTCAGC
CTCCTCTGCACCCTGGGCTTTGACTCTGTCAGGAAGAGAATGTCTGTGGTTGTGAGGCACCCACTGACT
GGCGAGATTGTTGTCTACACCAAGGGTGCTGACTCGGTCATCATGGACCTGCTGGAAGACCCAGCCTGC
GTACCTGACATTAATATGGAAAAGAAGCTGAGAAAAATCCGAGCCCGGACCCAAAAGCATCTAGACTTG
TATGCAAGAGATGGCCTGCGCACACTATGCATTGCCAAGAAGGTTGTAAGCGAAGAGGACTTCCGGAGA
TGGGCCAGTTTCCGGCGTGAGGCTGAGGCATCCCTCGACAACCGAGATGAGCTTCTCATGGAAACTGCA
CAGCATCTGGAGAATCAACTCACCTTACTTGGAGCCACTGGGATCGAAGACCGGCTGCAGGAAGGAGTT
CCAGATACGATTGCCACTCTGCGGGAGGCTGGGATCCAGCTCTGGGTCCTGACTGGAGATAAGCAGGAG
ACAGCGGTCAACATTGCCCATTCCTGCAGACTGTTAAATCAGACCGACACTGTTTATACCATCAATACA
GAGAATCAGGAGACCTGTGAATCCATCCTCAATTGTGCATTGGAAGAGCTAAAGCAATTTCGTGAACTA
CAGAAGCCAGACCGCAAGCTCTTTGGATTCCGCTTACCTTCCAAGACACCATCCATCACCTCAGAAGCT
GTGGTTCCAGAAGCTGGATTGGTCATCGATGGGAAGACATTGAATGCCATCTTCCAGGGAAAGCTAGAG
AAGAAGTTTCTGGAATTGACCCAGTATTGTCGGTCCGTCCTGTGCTGCCGCTCCACGCCACTCCAGAAG
AGTATGATAGTCAAGCTGGTGCGAGACAAGTTGCGCGTCATGACCCTTTCCATAGGTGATGGAGCAAAT
GATGTAAGCATGATTCAAGCTGCTGATATTGGAATTGGAATATCTGGACAGGAAGGCATGCAGGCTGTC
ATGTCCAGCGACTTTGCCATCACCCGCTTTAAGCATCTCAAGAAGTTGCTGCTCGTGCATGGCCACTGG
TGTTACTCGCGCCTGGCCAGGATGGTGGTGTACTACCTCTACAAGAACGTGTGCTACGTCAACCTGCTC
TTCTGGTATCAGTTCTTCTGTGGTTTCTCCAGCTCCACCATGATTGATTACTGGCAGATGATATTCTTC
AATCTCTTCTTTACCTCCTTGCCTCCTCTTGTCTTTGGAGTCCTTGACAAAGACATCTCTGCAGAAACA
CTCCTGGCATTGCCTGAGCTATACAAGAGTGGCCAGAACTCTGAGTGCTATAACCTGTCGACTTTCTGG
ATTTCTATGGTGGATGCATTCTACCAGAGCCTCATCTGTTTCTTTATCCCTTACCTGGCCTATAAGGGC
TCTGATATAGATGTCTTTACCTTTGGGACACCAATCAACACCATCTCCCTCACCACAATCCTTTTGCAC
CAGGCAATGGAAATGAAGACATGGACCATTTTCCACGGAGTCGTGCTCCTCGGCAGCTTCCTGATGTAC
TTTCTGGTATCCCTCCTGTACAATGCCACCTGCGTCATCTGCAACAGCCCCACCAATCCCTATTGGGTG
ATGGAAGGCCAGCTCTCAAACCCCACTTTCTACCTCGTCTGCTTTCTCACACCAGTTGTTGCTCTTCTC
CCAAGATACTTTTTCCTGTCTCTGCAAGGAACTTGTGGGAAGTCTCTAATCTCAAAAGCTCAGAAAATT
GACAAACTCCCCCCAGACAAAAGAAACCTGGAAATCCAGAGTTGGAGAAGCAGACAGAGGCCTGCCCCT
GTCCCCGAAGTGGCTCGACCAACTCACCACCCAGTGTCATCTATCACAGGACAGGACTTCAGTGCCAGC
ACCCCAAAGAGCTCTAACCCTCCCAAGAGGAAGCATGTGGAAGAGTCAGTACTCCACGAACAGAGATGT
GGCACGGAGTGCATGAGGGATGACTCATGCTCAGGGGACTCCTCAGCTCAACTCTCATCCGGGGAGCAC
CTGCTGGGACCTAACAGGATAATGGCCTACTCAAGAGGACAGACTGATATGTGCCGGTGCTCAAAGAGG
AGCAGCCATCGCCGATCCCAGAGTTCACTGACCATATGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_025153
Insert Size 4386 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025153.2
RefSeq Size 7582 bp
RefSeq ORF 4386 bp
Locus ID 23120
UniProt ID O94823
Cytogenetics 5q34
Protein Families Transmembrane
MW 165.4 kDa
Summary Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (Probable).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025153" in other vectors (4)
SKU Description Size Price
RC222292 ATP10B (Myc-DDK-tagged)-Human ATPase, class V, type 10B (ATP10B) 10 ug
$589.00 $1,250.00
RC222292L3 Lenti ORF clone of Human ATPase, class V, type 10B (ATP10B), Myc-DDK-tagged 10 ug
$1,550.00
RC222292L4 Lenti ORF clone of Human ATPase, class V, type 10B (ATP10B), mGFP tagged 10 ug
$1,550.00
RG222292 ATP10B (tGFP-tagged) - Human ATPase, class V, type 10B (ATP10B) 10 ug
$1,450.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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