MBD5 (NM_018328) Human Tagged ORF Clone

SKU RC222193

MBD5 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 5 (MBD5)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MBD5
• Synonyms: MRD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222193 representing NM_018328; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAATGGAGGCAAAGAGTGTGACGGAGGGGACAAGGAAGGAGGTCTTCCAGCTATACAAGTTCCTGTGG; GTTGGCAGCGTCGTGTGGATCAAAATGGAGTGCTTTATGTCAGTCCCAGTGGGTCTTTGTTATCTTGCTT; GGAGCAGGTTAAAACATACCTGCTTACTGATGGAACATGCAAGTGTGGCTTGGAATGTCCTCTTATTCTT; CCCAAGGTATTTAATTTTGATCCTGGAGCTGCTGTGAAACAGAGAACCGCAGAAGATGTTAAGGCAGATG; AAGATGTCACAAAGCTATGCATACATAAAAGAAAAATTATTGCAGTGGCCACACTTCATAAAAGCATGGA; AGCCCCACATCCTTCTCTGGTGCTCACCAGTCCCGGAGGAGGAACAAATGCAACTCCAGTAGTACCTTCT; CGGGCAGCAACTCCAAGATCAGTAAGAAATAAGTCTCATGAAGGAATTACAAATTCTGTAATGCCTGAAT; GTAAGAATCCTTTCAAGTTAATGATTGGATCATCAAATGCCATGGGAAGGCTATATGTACAAGAACTGCC; TGGAAGCCAACAACAAGAACTCCACCCTGTCTACCCCCGACAGAGATTGGGCAGCAGTGAACATGGACAG; AAATCTCCATTCCGTGGCAGCCATGGAGGCCTGCCCAGCCCAGCGTCATCAGGTTCCCAGATATATGGAG; ATGGTTCAATCTCTCCAAGGACTGACCCACTTGGAAGTCCTGATGTTTTCACAAGAAGTAATCCTGGTTT; TCATGGAGCTCCCAATTCTAGTCCTATTCACCTGAATAGGACTCCTCTTTCTCCACCTTCAGTAATGCTA; CATGGTTCTCCTGTACAGTCATCCTGTGCAATGGCTGGAAGGACTAATATACCTCTTTCCCCAACCTTGA; CTACAAAGAGTCCAGTAATGAAAAAACCAATGTGTAATTTTTCAACTAATATGGAAATACCACGAGCAAT; GTTCCACCACAAACCACCCCAAGGCCCACCTCCCCCTCCTCCACCTTCTTGTGCTCTTCAGAAAAAGCCA; TTAACATCTGAGAAAGATCCACTTGGCATTCTTGACCCTATTCCTAGTAAACCAGTGAATCAGAACCCTG; TTATCATTAATCCAACCAGTTTCCATTCAAATGTCCACTCTCAGGTACCTATGATGAATGTAAGCATGCC; TCCTGCTGTTGTTCCTTTGCCAAGTAATCTCCCATTGCCAACTGTAAAACCTGGTCACATGAATCATGGG; AGTCATGTACAAAGAGTTCAGCATTCAGCTTCAACCTCCCTGTCCCCTTCTCCAGTGACATCCCCCGTGC; ACATGATGGGGACTGGAATTGGAAGGATTGAGGCATCGCCCCAAAGATCACGCTCATCTTCCACATCATC; AGATCATGGAAATTTCATGATGCCACCTGTAGGACCCCAGGCCACTTCTAGTGGTATTAAGGTTCCACCC; AGGTCACCAAGGTCAACAATAGGGTCCCCAAGGCCATCAATGCCATCAAGCCCTTCTACCAAGTCCGATG; GACATCATCAGTACAAGGATATCCCTAACCCATTAATTGCTGGAATAAGTAATGTACTAAATACCCCAAG; CAGTGCAGCTTTTCCTACTGCATCTGCCGGAAGTAGTTCTGTAAAGAGTCAGCCTGGTTTGCTGGGAATG; CCTTTAAATCAGATCTTGAACCAGCACAATGCTGCCTCCTTTCCAGCAAGTAGTTTACTCTCAGCAGCAG; CCAAAGCACAGCTAGCAAATCAAAACAAACTTGCTGGTAACAACAGTAGCAGCAGTAGCAATTCTGGAGC; TGTTGCCGGCAGTGGCAACACTGAAGGACATAGCACTTTAAACACCATGTTCCCTCCTACTGCCAACATG; CTTCTCCCAACAGGTGAAGGGCAAAGTGGTCGAGCAGCACTAAGAGATAAGCTGATGTCTCAGCAAAAAG; ACGCATTGCGGAAAAGAAAACAACCACCTACGACAGTGTTGAGTTTGCTCAGACAGTCTCAAATGGATAG; TTCTGCAGTTCCTAAACCTGGACCTGACTTGCTAAGGAAGCAGGGTCAGGGTTCATTTCCCATCAGTTCA; ATGTCTCAGTTACTACAGTCTATGAGTTGTCAAAGCTCTCACTTGAGTAGCAATAGTACCCCGGGTTGTG; GGGCCTCAAATACTGCTTTGCCTTGCTCTGCTAACCAGCTGCATTTTACAGATCCCAGTATGAACTCTAG; TGTTCTTCAGAACATACCTTTAAGAGGGGAAGCCGTGCACTGCCACAATGCAAACACTAACTTTGTTCAC; AGTAACAGTCCAGTCCCCAACCACCATCTTGCAGGTTTAATAAATCAGATTCAGGCTAGCGGGAACTGTG; GGATGCTCAGTCAGTCGGGCATGGCTTTAGGAAATTCCTTACATCCCAATCCACCTCAGTCAAGAATTTC; AACGTCCTCCACTCCAGTGATACCAAACAGCATTGTTAGCAGCTATAATCAAACAAGTTCTGAAGCAGGC; GGTTCAGGACCATCATCCTCCATAGCCATAGCGGGCACCAACCACCCTGCCATCACAAAGACAACATCTG; TTCTTCAAGATGGCGTCATAGTCACCACTGCAGCTGGAAACCCACTGCAGAGTCAGCTACCCATTGGGAG; TGATTTTCCTTTTGTTGGCCAGGAGCACGCACTTCATTTTCCATCCAACAGCACTTCAAACAACCATCTT; CCACACCCCTTGAACCCCAGCCTCCTCAGTTCTCTACCTATCTCTTTGCCAGTGAATCAACAGCATCTCC; TAAACCAGAATCTATTAAATATCCTCCAGCCTTCAGCAGGAGAAGGTGATATGTCATCAATAAACAATAC; TTTGAGTAACCATCAACTGACTCATCTACAGTCGCTGTTAAACAACAATCAGATGTTTCCTCCAAATCAG; CAACAGCAGCAACTTCTCCAGGGGTACCAGAATCTCCAGGCGTTCCAAGGACAGTCCACAATTCCTTGCC; CAGCTAACAATAACCCCATGGCTTGTCTGTTTCAGAACTTTCAGGTGAGAATGCAGGAAGATGCAGCTCT; CCTAAACAAAAGAATAAGCACTCAGCCTGGGCTCACAGCACTTCCTGAGAATCCAAACACTACACTTCCA; CCTTTTCAAGATACACCTTGTGAGTTGCAACCGAGGATTGACCCATCTCTTGGTCAACAGGTGAAGGATG; GCCTCGTTGTGGGTGGCCCAGGTGATGCTTCCGTAGATGCCATTTACAAAGCAGTTGTCGATGCAGCCAG; CAAAGGAATGCAGGTTGTCATCACCACTGCAGTCAACAGTACAACTCAGATCAGCCCCATTCCAGCTCTG; AGTGCCATGAGTGCCTTCACTGCCTCAATTGGTGACCCATTAAATCTCTCCAGTGCTGTCAGTGCGGTCA; TTCATGGACGGAACATGGGAGGTGTTGATCATGATGGTAGGCTGAGGAATTCAAGAGGGGCTCGGCTGCC; CAAGAATCTAGACCATGGGAAAAATGTGAACGAAGGAGATGGGTTTGAATATTTCAAGTCAGCAAGTTGC; CACACATCCAAAAAACAGTGGGACGGGGAGCAAAGCCCCAGAGGGGAGCGAAACAGGTGGAAGTACGAGG; AATTTTTAGATCATCCAGGCCATATCCACAGTAGTCCTTGTCATGAAAGGCCCAACAATGTCTCTACACT; GCCATTTCTGCCTGGGGAACAGCACCCAATACTGTTACCACCAAGAAACTGTCCAGGGGATAAAATTCTA; GAGGAAAATTTCAGGTATAATAACTACAAAAGAACTATGATGAGTTTTAAGGAGAGACTAGAGAACACTG; TGGAAAGATGTGCACACATAAATGGGAATAGACCTCGACAGAGTCGGGGATTTGGAGAGCTGCTAAGCAC; TGCAAAGCAAGACCTGGTCCTAGAGGAGCAGTCTCCAAGTTCCTCAAATAGTTTGGAAAATTCTCTGGTC; AAAGACTACATCCATTACAATGGAGACTTTAATGCCAAAAGCGTTAATGGGTGTGTGCCTAGCCCTTCAG; ATGCTAAAAGCATTAGTAGTGAAGATGACCTAAGGAACCCAGACTCCCCCTCTTCAAATGAATTGATACA; TTATAGACCAAGGACGTTCAATGTTGGCGACTTGGTCTGGGGCCAAATCAAAGGACTGACTTCCTGGCCT; GGAAAATTAGTAAGAGAAGACGACGTTCACAATTCATGTCAACAAAGCCCCGAGGAAGGGAAGGTGGAGC; CCGAGAAGTTGAAGACACTAACAGAAGGTTTGGAAGCCTACAGCCGTGTCCGGAAAAGGAACAGAAAAAG; TGGAAAGCTAAATAACCATTTAGAAGCTGCTATTCATGAGGCCATGAGTGAACTGGACAAAATGTCTGGG; ACTGTACACCAAATCCCACAGGGTGACAGACAAATGAGACCCCCCAAACCCAAGAGGAGGAAGATCTCCA; GA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222193 representing NM_018328; Red=Cloning site Green=Tags(s); ; MNGGKECDGGDKEGGLPAIQVPVGWQRRVDQNGVLYVSPSGSLLSCLEQVKTYLLTDGTCKCGLECPLIL; PKVFNFDPGAAVKQRTAEDVKADEDVTKLCIHKRKIIAVATLHKSMEAPHPSLVLTSPGGGTNATPVVPS; RAATPRSVRNKSHEGITNSVMPECKNPFKLMIGSSNAMGRLYVQELPGSQQQELHPVYPRQRLGSSEHGQ; KSPFRGSHGGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRSNPGFHGAPNSSPIHLNRTPLSPPSVML; HGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQGPPPPPPPSCALQKKP; LTSEKDPLGILDPIPSKPVNQNPVIINPTSFHSNVHSQVPMMNVSMPPAVVPLPSNLPLPTVKPGHMNHG; SHVQRVQHSASTSLSPSPVTSPVHMMGTGIGRIEASPQRSRSSSTSSDHGNFMMPPVGPQATSSGIKVPP; RSPRSTIGSPRPSMPSSPSTKSDGHHQYKDIPNPLIAGISNVLNTPSSAAFPTASAGSSSVKSQPGLLGM; PLNQILNQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSSNSGAVAGSGNTEGHSTLNTMFPPTANM; LLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISS; MSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVH; SNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAG; GSGPSSSIAIAGTNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTSNNHL; PHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGDMSSINNTLSNHQLTHLQSLLNNNQMFPPNQ; QQQQLLQGYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRISTQPGLTALPENPNTTLP; PFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKAVVDAASKGMQVVITTAVNSTTQISPIPAL; SAMSAFTASIGDPLNLSSAVSAVIHGRNMGGVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASC; HTSKKQWDGEQSPRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKIL; EENFRYNNYKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLENSLV; KDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVGDLVWGQIKGLTSWP; GKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRVRKRNRKSGKLNNHLEAAIHEAMSELDKMSG; TVHQIPQGDRQMRPPKPKRRKISR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018328
• ORF Size: 4482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018328.5
• RefSeq Size: 5325 bp
• RefSeq ORF: 4485 bp
• Locus ID: 55777
• UniProt ID: Q9P267
• Cytogenetics: 2q23.1
• MW: 159.7 kDa
• Summary: This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain, this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif), which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division, growth and differentiation. Mutations in this gene cause an autosomal dominant type of cognitive disability. The encoded protein interacts with the polycomb repressive complex PR-DUB which catalyzes the deubiquitination of a lysine residue of histone 2A. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly, intellectual disabilities, severe speech impairment, and seizures. Alternatively spliced transcript variants have been found, but their full-length nature is not determined. [provided by RefSeq, Jul 2017]