OATP2 (SLCO1B1) (NM_006446) Human Tagged ORF Clone

SKU RC222130

SLCO1B1 (Myc-DDK-tagged)-Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: OATP2
• Synonyms: HBLRR; LST-1; LST1; OATP-C; OATP1B1; OATP2; OATPC; SLC21A6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222130 representing NM_006446; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGACCAAAATCAACATTTGAATAAAACAGCAGAGGCACAACCTTCAGAGAATAAGAAAACAAGATACT; GCAATGGATTGAAGATGTTCTTGGCAGCTCTGTCACTCAGCTTTATTGCTAAGACACTAGGTGCAATTAT; TATGAAAAGTTCCATCATTCATATAGAACGGAGATTTGAGATATCCTCTTCTCTTGTTGGTTTTATTGAC; GGAAGCTTTGAAATTGGAAATTTGCTTGTGATTGTATTTGTGAGTTACTTTGGATCCAAACTACATAGAC; CAAAGTTAATTGGAATCGGTTGTTTCATTATGGGAATTGGAGGTGTTTTGACTGCTTTGCCACATTTCTT; CATGGGATATTACAGGTATTCTAAAGAAACTAATATCGATTCATCAGAAAATTCAACATCGACCTTATCC; ACTTGTTTAATTAATCAAATTTTATCACTCAATAGAGCATCACCTGAGATAGTGGGAAAAGGTTGTTTAA; AGGAATCTGGGTCATACATGTGGATATATGTGTTCATGGGTAATATGCTTCGTGGAATAGGGGAGACTCC; CATAGTACCATTGGGGCTTTCTTACATTGATGATTTCGCTAAAGAAGGACATTCTTCTTTGTATTTAGGT; ATATTGAATGCAATAGCAATGATTGGTCCAATCATTGGCTTTACCCTGGGATCTCTGTTTTCTAAAATGT; ACGTGGATATTGGATATGTAGATCTAAGCACTATCAGGATAACTCCTACTGATTCTCGATGGGTTGGAGC; TTGGTGGCTTAATTTCCTTGTGTCTGGACTATTCTCCATTATTTCTTCCATACCATTCTTTTTCTTGCCC; CAAACTCCAAATAAACCACAAAAAGAAAGAAAAGCTTCACTGTCTTTGCATGTGCTGGAAACAAATGATG; AAAAGGATCAAACAGCTAATTTGACCAATCAAGGAAAAAATATTACCAAAAATGTGACTGGTTTTTTCCA; GTCTTTTAAAAGCATCCTTACTAATCCCCTGTATGTTATGTTTGTGCTTTTGACGTTGTTACAAGTAAGC; AGCTATATTGGTGCTTTTACTTATGTCTTCAAATACGTAGAGCAACAGTATGGTCAGCCTTCATCTAAGG; CTAACATCTTATTGGGAGTCATAACCATACCTATTTTTGCAAGTGGAATGTTTTTAGGAGGATATATCAT; TAAAAAATTCAAACTGAACACCGTTGGAATTGCCAAATTCTCATGTTTTACTGCTGTGATGTCATTGTCC; TTTTACCTATTATATTTTTTCATACTCTGTGAAAACAAATCAGTTGCCGGACTAACCATGACCTATGATG; GAAATAATCCAGTGACATCTCATAGAGATGTACCACTTTCTTATTGCAACTCAGACTGCAATTGTGATGA; AAGTCAATGGGAACCAGTCTGTGGAAACAATGGAATAACTTACATCTCACCCTGTCTAGCAGGTTGCAAA; TCTTCAAGTGGCAATAAAAAGCCTATAGTGTTTTACAACTGCAGTTGTTTGGAAGTAACTGGTCTCCAGA; ACAGAAATTACTCAGCCCATTTGGGTGAATGCCCAAGAGATGATGCTTGTACAAGGAAATTTTACTTTTT; TGTTGCAATACAAGTCTTGAATTTATTTTTCTCTGCACTTGGAGGCACCTCACATGTCATGCTGATTGTT; AAAATTGTTCAACCTGAATTGAAATCACTTGCACTGGGTTTCCACTCAATGGTTATACGAGCACTAGGAG; GAATTCTAGCTCCAATATATTTTGGGGCTCTGATTGATACAACGTGTATAAAGTGGTCCACCAACAACTG; TGGCACACGTGGGTCATGTAGGACATATAATTCCACATCATTTTCAAGGGTCTACTTGGGCTTGTCTTCA; ATGTTAAGAGTCTCATCACTTGTTTTATATATTATATTAATTTATGCCATGAAGAAAAAATATCAAGAGA; AAGATATCAATGCATCAGAAAATGGAAGTGTCATGGATGAAGCAAACTTAGAATCCTTAAATAAAAATAA; ACATTTTGTCCCTTCTGCTGGGGCAGATAGTGAAACACATTGT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222130 representing NM_006446; Red=Cloning site Green=Tags(s); ; MDQNQHLNKTAEAQPSENKKTRYCNGLKMFLAALSLSFIAKTLGAIIMKSSIIHIERRFEISSSLVGFID; GSFEIGNLLVIVFVSYFGSKLHRPKLIGIGCFIMGIGGVLTALPHFFMGYYRYSKETNIDSSENSTSTLS; TCLINQILSLNRASPEIVGKGCLKESGSYMWIYVFMGNMLRGIGETPIVPLGLSYIDDFAKEGHSSLYLG; ILNAIAMIGPIIGFTLGSLFSKMYVDIGYVDLSTIRITPTDSRWVGAWWLNFLVSGLFSIISSIPFFFLP; QTPNKPQKERKASLSLHVLETNDEKDQTANLTNQGKNITKNVTGFFQSFKSILTNPLYVMFVLLTLLQVS; SYIGAFTYVFKYVEQQYGQPSSKANILLGVITIPIFASGMFLGGYIIKKFKLNTVGIAKFSCFTAVMSLS; FYLLYFFILCENKSVAGLTMTYDGNNPVTSHRDVPLSYCNSDCNCDESQWEPVCGNNGITYISPCLAGCK; SSSGNKKPIVFYNCSCLEVTGLQNRNYSAHLGECPRDDACTRKFYFFVAIQVLNLFFSALGGTSHVMLIV; KIVQPELKSLALGFHSMVIRALGGILAPIYFGALIDTTCIKWSTNNCGTRGSCRTYNSTSFSRVYLGLSS; MLRVSSLVLYIILIYAMKKKYQEKDINASENGSVMDEANLESLNKNKHFVPSAGADSETHC; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006446
• ORF Size: 2073 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006446.3
• RefSeq Size: 2830 bp
• RefSeq ORF: 2076 bp
• Locus ID: 10599
• UniProt ID: Q9Y6L6
• Cytogenetics: 12p12.1
• Domains: OATP_C, OATP_N
• Protein Families: Druggable Genome, Transmembrane
• MW: 76.3 kDa
• Summary: This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of numerous endogenous compounds including bilirubin, 17-beta-glucuronosyl estradiol and leukotriene C4. This protein is also involved in the removal of drug compounds such as statins, bromosulfophthalein and rifampin from the blood into the hepatocytes. Polymorphisms in the gene encoding this protein are associated with impaired transporter function. [provided by RefSeq, Mar 2009]