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OATP2 (SLCO1B1) (NM_006446) Human Tagged ORF Clone

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SKU
RC222130
SLCO1B1 (Myc-DDK-tagged)-Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $633.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OATP2
Synonyms HBLRR; LST-1; LST1; OATP-C; OATP1B1; OATP2; OATPC; SLC21A6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222130 representing NM_006446
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCAAAATCAACATTTGAATAAAACAGCAGAGGCACAACCTTCAGAGAATAAGAAAACAAGATACT
GCAATGGATTGAAGATGTTCTTGGCAGCTCTGTCACTCAGCTTTATTGCTAAGACACTAGGTGCAATTAT
TATGAAAAGTTCCATCATTCATATAGAACGGAGATTTGAGATATCCTCTTCTCTTGTTGGTTTTATTGAC
GGAAGCTTTGAAATTGGAAATTTGCTTGTGATTGTATTTGTGAGTTACTTTGGATCCAAACTACATAGAC
CAAAGTTAATTGGAATCGGTTGTTTCATTATGGGAATTGGAGGTGTTTTGACTGCTTTGCCACATTTCTT
CATGGGATATTACAGGTATTCTAAAGAAACTAATATCGATTCATCAGAAAATTCAACATCGACCTTATCC
ACTTGTTTAATTAATCAAATTTTATCACTCAATAGAGCATCACCTGAGATAGTGGGAAAAGGTTGTTTAA
AGGAATCTGGGTCATACATGTGGATATATGTGTTCATGGGTAATATGCTTCGTGGAATAGGGGAGACTCC
CATAGTACCATTGGGGCTTTCTTACATTGATGATTTCGCTAAAGAAGGACATTCTTCTTTGTATTTAGGT
ATATTGAATGCAATAGCAATGATTGGTCCAATCATTGGCTTTACCCTGGGATCTCTGTTTTCTAAAATGT
ACGTGGATATTGGATATGTAGATCTAAGCACTATCAGGATAACTCCTACTGATTCTCGATGGGTTGGAGC
TTGGTGGCTTAATTTCCTTGTGTCTGGACTATTCTCCATTATTTCTTCCATACCATTCTTTTTCTTGCCC
CAAACTCCAAATAAACCACAAAAAGAAAGAAAAGCTTCACTGTCTTTGCATGTGCTGGAAACAAATGATG
AAAAGGATCAAACAGCTAATTTGACCAATCAAGGAAAAAATATTACCAAAAATGTGACTGGTTTTTTCCA
GTCTTTTAAAAGCATCCTTACTAATCCCCTGTATGTTATGTTTGTGCTTTTGACGTTGTTACAAGTAAGC
AGCTATATTGGTGCTTTTACTTATGTCTTCAAATACGTAGAGCAACAGTATGGTCAGCCTTCATCTAAGG
CTAACATCTTATTGGGAGTCATAACCATACCTATTTTTGCAAGTGGAATGTTTTTAGGAGGATATATCAT
TAAAAAATTCAAACTGAACACCGTTGGAATTGCCAAATTCTCATGTTTTACTGCTGTGATGTCATTGTCC
TTTTACCTATTATATTTTTTCATACTCTGTGAAAACAAATCAGTTGCCGGACTAACCATGACCTATGATG
GAAATAATCCAGTGACATCTCATAGAGATGTACCACTTTCTTATTGCAACTCAGACTGCAATTGTGATGA
AAGTCAATGGGAACCAGTCTGTGGAAACAATGGAATAACTTACATCTCACCCTGTCTAGCAGGTTGCAAA
TCTTCAAGTGGCAATAAAAAGCCTATAGTGTTTTACAACTGCAGTTGTTTGGAAGTAACTGGTCTCCAGA
ACAGAAATTACTCAGCCCATTTGGGTGAATGCCCAAGAGATGATGCTTGTACAAGGAAATTTTACTTTTT
TGTTGCAATACAAGTCTTGAATTTATTTTTCTCTGCACTTGGAGGCACCTCACATGTCATGCTGATTGTT
AAAATTGTTCAACCTGAATTGAAATCACTTGCACTGGGTTTCCACTCAATGGTTATACGAGCACTAGGAG
GAATTCTAGCTCCAATATATTTTGGGGCTCTGATTGATACAACGTGTATAAAGTGGTCCACCAACAACTG
TGGCACACGTGGGTCATGTAGGACATATAATTCCACATCATTTTCAAGGGTCTACTTGGGCTTGTCTTCA
ATGTTAAGAGTCTCATCACTTGTTTTATATATTATATTAATTTATGCCATGAAGAAAAAATATCAAGAGA
AAGATATCAATGCATCAGAAAATGGAAGTGTCATGGATGAAGCAAACTTAGAATCCTTAAATAAAAATAA
ACATTTTGTCCCTTCTGCTGGGGCAGATAGTGAAACACATTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222130 representing NM_006446
Red=Cloning site Green=Tags(s)

MDQNQHLNKTAEAQPSENKKTRYCNGLKMFLAALSLSFIAKTLGAIIMKSSIIHIERRFEISSSLVGFID
GSFEIGNLLVIVFVSYFGSKLHRPKLIGIGCFIMGIGGVLTALPHFFMGYYRYSKETNIDSSENSTSTLS
TCLINQILSLNRASPEIVGKGCLKESGSYMWIYVFMGNMLRGIGETPIVPLGLSYIDDFAKEGHSSLYLG
ILNAIAMIGPIIGFTLGSLFSKMYVDIGYVDLSTIRITPTDSRWVGAWWLNFLVSGLFSIISSIPFFFLP
QTPNKPQKERKASLSLHVLETNDEKDQTANLTNQGKNITKNVTGFFQSFKSILTNPLYVMFVLLTLLQVS
SYIGAFTYVFKYVEQQYGQPSSKANILLGVITIPIFASGMFLGGYIIKKFKLNTVGIAKFSCFTAVMSLS
FYLLYFFILCENKSVAGLTMTYDGNNPVTSHRDVPLSYCNSDCNCDESQWEPVCGNNGITYISPCLAGCK
SSSGNKKPIVFYNCSCLEVTGLQNRNYSAHLGECPRDDACTRKFYFFVAIQVLNLFFSALGGTSHVMLIV
KIVQPELKSLALGFHSMVIRALGGILAPIYFGALIDTTCIKWSTNNCGTRGSCRTYNSTSFSRVYLGLSS
MLRVSSLVLYIILIYAMKKKYQEKDINASENGSVMDEANLESLNKNKHFVPSAGADSETHC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006446
ORF Size 2073 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006446.3
RefSeq Size 2830 bp
RefSeq ORF 2076 bp
Locus ID 10599
UniProt ID Q9Y6L6, A0A024RAU7, Q05CV5
Cytogenetics 12p12.1
Domains OATP_C, OATP_N
Protein Families Druggable Genome, Transmembrane
MW 76.3 kDa
Summary This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of numerous endogenous compounds including bilirubin, 17-beta-glucuronosyl estradiol and leukotriene C4. This protein is also involved in the removal of drug compounds such as statins, bromosulfophthalein and rifampin from the blood into the hepatocytes. Polymorphisms in the gene encoding this protein are associated with impaired transporter function. provided by RefSeq, Mar 2009
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"NM_006446" in other vectors (6)
SKU Description Size Price
RC222130L1 Lenti ORF clone of Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1), Myc-DDK-tagged 10 ug
$933.00
RC222130L2 Lenti ORF clone of Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1), mGFP tagged 10 ug
$933.00
RC222130L3 Lenti ORF clone of Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1), Myc-DDK-tagged 10 ug
$933.00
RC222130L4 Lenti ORF clone of Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1), mGFP tagged 10 ug
$933.00
RG222130 SLCO1B1 (tGFP-tagged) - Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1) 10 ug
$789.00 $833.00
SC116071 SLCO1B1 (untagged)-Human solute carrier organic anion transporter family, member 1B1 (SLCO1B1) 10 ug
$950.00

Plasmid Map for RC222130

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222130
Circular map for RC222130

file-alt Citations

Characterization of Plasma Membrane Localization and Phosphorylation Status of Organic Anion Transporting Polypeptide (OATP) 1B1 c.521 T>C Nonsynonymous Single-Nucleotide Polymorphism
Crowe, Zheng, Miller et al
Pharm. Res. (2019) 36 (7), 101 DOI: 10.1007/s11095-019-2634-3
AI Summary: OriGene contributed to this study by providing the pCMV6-FLAG-V174A-OATP1B1 mammalian plasmid expression vector, which was used to express the V174A-OATP1B1 protein for functional and localization analyses.
Snippet: S II. Construct and Transfection. A mammalian plasmid expression vector encoding C-terminus FLAG-tagged V174A-OATP1B1 (pCMV6-FLAG-V174A-OATP1B1) was constructed at Origene (Rockville, MD) and was verified by sequencing
A phosphotyrosine switch regulates organic cation transporters
Sprowl, Ong, Gibson et al
Nat Commun (2016) 7, 10880 DOI: 10.1038/ncomms10880
Cited by 142
AI Summary: OriGene contributed to this study by providing the pCMV6-Entry (C-terminal FLAG tagged) plasmids for OCT1, OCT2, OCT3, MATE1, OATP1B1, YES1, LYN, and KDR, which were used for mutagenesis and transient transfection experiments to investigate phosphorylation-dependent regulation of drug transporters.
Snippet: The OCT1, OCT2, OCT3, MATE1, OATP1B1, YES1, LYN and KDR plasmids with pCMV6-Entry (C-terminal FLAG tagged) backbone were obtained from Origene. The QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) was utilized to generate mutants, according to suggested methods. …
FigureFigureFigure
Down-Regulation of Organic Anion Transporting Polypeptide (OATP) 1B1 Transport Function by Lysosomotropic Drug Chloroquine
Alam, Pahwa, Wang et al
Molecular pharmarceutics (2016)
Snippet: established by 145 transfection of a mammalian plasmid expression vector encoding C-terminus FLAG-tagged 146 OATP1B1 (pCMV6-FLAG-OATP1B1) that was custom constructed through Origene (Rockville, 147 MD). Both cell ... Cite SaveSaving
Showing 1-3 of 3 papers.
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