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MITF (NM_198178) Human Tagged ORF Clone

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SKU
RC222074
MITF (Myc-DDK-tagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 6
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "MITF or NM_198178"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MITF
Synonyms bHLHe32; CMM8; COMMAD; MI; WS2; WS2A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222074 representing NM_198178
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGAAATGCTAGAATATAATCACTATCAGGTGCAGACCCACCTCGAAAACCCCACCAAGTACCACA
TACAGCAAGCCCAACGGCAGCAGGGATTTTATAAGTTTGAAGAGCAAAACAGGGCAGAGAGCGAGTGCCC
AGGCATGAACACACATTCACGAGCGTCCTGTATGCAGATGGATGATGTAATCGATGACATCATTAGCCTA
GAATCAAGTTATAATGAGGAAATCTTGGGCTTGATGGATCCTGCTTTGCAAATGGCAAATACGTTGCCTG
TCTCGGGAAACTTGATTGATCTTTATGGAAACCAAGGTCTGCCCCCACCAGGCCTCACCATCAGCAACTC
CTGTCCAGCCAACCTTCCCAACATAAAAAGGGAGCTCACAGAGTCTGAAGCAAGAGCACTGGCCAAAGAG
AGGCAGAAAAAGGACAATCACAACCTGATTGAACGAAGAAGAAGATTTAACATAAATGACCGCATTAAAG
AACTAGGTACTTTGATTCCCAAGTCAAATGATCCAGACATGCGCTGGAACAAGGGAACCATCTTAAAAGC
ATCCGTGGACTATATCCGAAAGTTGCAACGAGAACAGCAACGCGCAAAAGAACTTGAAAACCGACAGAAG
AAACTGGAGCACGCCAACCGGCATTTGTTGCTCAGAATACAGGAACTTGAAATGCAGGCTCGAGCTCATG
GACTTTCCCTTATTCCATCCACGGGTCTCTGCTCTCCAGATTTGGTGAATCGGATCATCAAGCAAGAACC
CGTTCTTGAGAACTGCAGCCAAGACCTCCTTCAGCATCATGCAGACCTAACCTGTACAACAACTCTCGAT
CTCACGGATGGCACCATCACCTTCAACAACAACCTCGGAACTGGGACTGAGGCCAACCAAGCCTATAGTG
TCCCCACAAAAATGGGATCCAAACTGGAAGACATCCTGATGGACGACACCCTTTCTCCCGTCGGTGTCAC
TGATCCACTCCTTTCCTCAGTGTCCCCCGGAGCTTCCAAAACAAGCAGCCGGAGGAGCAGTATGAGCATG
GAAGAGACGGAGCACACTTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222074 representing NM_198178
Red=Cloning site Green=Tags(s)

MLEMLEYNHYQVQTHLENPTKYHIQQAQRQQGFYKFEEQNRAESECPGMNTHSRASCMQMDDVIDDIISL
ESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQGLPPPGLTISNSCPANLPNIKRELTESEARALAKE
RQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKELENRQK
KLEHANRHLLLRIQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLD
LTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSM
EETEHTC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198178
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198178.3
RefSeq Size 4597 bp
RefSeq ORF 1074 bp
Locus ID 4286
UniProt ID O75030
Cytogenetics 3p13
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Melanogenesis, Melanoma, Pathways in cancer
MW 55.2 kDa
Summary The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. provided by RefSeq, Aug 2017
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"NM_198178" in other vectors (6)
SKU Description Size Price
RC222074L1 Lenti-ORF clone of MITF (Myc-DDK-tagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 6 10 ug
$757.00
RC222074L2 Lenti-ORF clone of MITF (mGFP-tagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 6 10 ug
$757.00
RC222074L3 Lenti-ORF clone of MITF (Myc-DDK-tagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 6 10 ug
$757.00
RC222074L4 Lenti-ORF clone of MITF (mGFP-tagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 6 10 ug
$757.00
RG222074 MITF (tGFP-tagged) - Human microphthalmia-associated transcription factor (MITF), transcript variant 6 10 ug
$657.00
SC307640 MITF (untagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 6 10 ug
$457.00

Plasmid Map for RC222074

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222074
Circular map for RC222074

file-alt Citations

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