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Neugrin (NGRN) (NM_001033088) Human Tagged ORF Clone

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SKU
RC222032
NGRN (Myc-DDK-tagged)-Human neugrin, neurite outgrowth associated (NGRN), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Neugrin
Synonyms DSC92
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222032 representing NM_001033088
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTTACCCTGAGTCTCTTGCTGGGCGGGCGCGTTTGCGCCGCCGTCACTCGCTGTGGGTTCGCGA
CCCGGGGGGTGGCGGGCCCAGGCCCTATTGGCCGGGAGCCGGACCCCGATTCCGACTGGGAGCCGGAGGA
ACGGGAGCTGCAGGAGGTGGAGAGCACCCTGAAACGACAGAAACAAGCAATCCGATTCCAGAAAATTCGG
AGGCAAATGGAGGCGCCTGGTGCCCCGCCCAGGACCCTGACGTGGGAAGCCATGGAGCAGATACGGTATT
TACATGAGGAATTTCCAGAGTCCTGGTCAGTTCCCAGGTTGGCTGAAGGCTTTGATGTCAGCACTGATGT
GATCCGAAGAGTTTTAAAAAGCAAGTTTTTACCCACATTGGAGCAGAAGCTGAAGCAGGATCAAAAAGTC
CTTAAGAAAGCTGGGCTTGCCCACTCGCTGCAGCACCTCCGGGGCTCTGGAAATACCTCAAAGCTGCTCC
CTGCAGGCCACTCTGTATCAGGCTCTTTGCTTATGCCAGGGCATGAAGCCTCATCTAAAGACCCAAATCA
CAGCACAGCTTTGAAAGTGATAGAGTCAGACACTCACAGGACAAATACACCAAGGAGAAGGAAGGGAAGA
AATAAAGAAATCCAGGACCTGGAGGAGAGCTTTGTGCCTGTTGCTGCACCCCTAGGTCATCCAAGAGAGC
TGCAGAAGTACTCCAGTGATTCTGAGAGCCCCAGAGGAACTGGCAGTGGTGCGTTGCCAAGTGGTCAGAA
GCTGGAGGAGTTGAAGGCAGAGGAGCCAGATAACTTCAGCAGCAAAGTAGTGCAGAGGGGCCGAGAGTTC
TTTGACAGCAACGGGAACTTCCTGTACAGAATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222032 representing NM_001033088
Red=Cloning site Green=Tags(s)

MAVTLSLLLGGRVCAAVTRCGFATRGVAGPGPIGREPDPDSDWEPEERELQEVESTLKRQKQAIRFQKIR
RQMEAPGAPPRTLTWEAMEQIRYLHEEFPESWSVPRLAEGFDVSTDVIRRVLKSKFLPTLEQKLKQDQKV
LKKAGLAHSLQHLRGSGNTSKLLPAGHSVSGSLLMPGHEASSKDPNHSTALKVIESDTHRTNTPRRRKGR
NKEIQDLEESFVPVAAPLGHPRELQKYSSDSESPRGTGSGALPSGQKLEELKAEEPDNFSSKVVQRGREF
FDSNGNFLYRI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033088
ORF Size 873 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033088.3
RefSeq Size 1367 bp
RefSeq ORF 876 bp
Locus ID 51335
UniProt ID Q9NPE2
Cytogenetics 15q26.1
MW 32.2 kDa
Summary Plays an essential role in mitochondrial ribosome biogenesis. As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation of core subunits of the oxidative phosphorylation system.UniProtKB/Swiss-Prot Function
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"NM_001033088" in other vectors (6)
SKU Description Size Price
RC222032L1 Lenti ORF clone of Human neugrin, neurite outgrowth associated (NGRN), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC222032L2 Lenti ORF clone of Human neugrin, neurite outgrowth associated (NGRN), transcript variant 2, mGFP tagged 10 ug
$600.00
RC222032L3 Lenti ORF clone of Human neugrin, neurite outgrowth associated (NGRN), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC222032L4 Lenti ORF clone of Human neugrin, neurite outgrowth associated (NGRN), transcript variant 2, mGFP tagged 10 ug
$600.00
RG222032 NGRN (tGFP-tagged) - Human neugrin, neurite outgrowth associated (NGRN), transcript variant 2 10 ug
$500.00
SC302701 NGRN (untagged)-Human neugrin, neurite outgrowth associated (NGRN), transcript variant 2 10 ug
$330.00

Plasmid Map for RC222032

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222032
Circular map for RC222032

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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