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PDE3A (NM_000921) Human Tagged ORF Clone

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SKU
RC222006
PDE3A (Myc-DDK-tagged)-Human phosphodiesterase 3A, cGMP-inhibited (PDE3A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $993.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDE3A
Synonyms CGI-PDE; CGI-PDE-A; CGI-PDE A; HTNB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222006 representing NM_000921
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGTGCCCGGCGACGCTGCACGAGTCAGGGACAAGCCCGTCCACAGTGGGGTGAGTCAAGCCCCCA
CGGCGGGCCGGGACTGCCACCATCGTGCGGACCCCGCATCGCCGCGGGACTCGGGCTGCCGTGGCTGCTG
GGGAGACCTGGTGCTGCAGCCGCTCCGGAGCTCTCGGAAACTTTCCTCCGCGCTGTGCGCGGGCTCCCTG
TCCTTTCTGCTGGCGCTGCTGGTGAGGCTGGTCCGCGGGGAGGTCGGCTGTGACCTGGAGCAGTGTAAGG
AGGCGGCGGCGGCGGAGGAGGAGGAAGCAGCCCCGGGAGCAGAAGGGGGCGTCTTCCCGGGGCCTCGGGG
AGGTGCTCCCGGGGGCGGTGCGCGGCTCAGCCCCTGGCTGCAGCCCTCGGCGCTGCTCTTCAGTCTCCTG
TGTGCCTTCTTCTGGATGGGCTTGTACCTCCTGCGCGCCGGGGTGCGCCTGCCTCTGGCTGTCGCGCTGC
TGGCCGCCTGCTGCGGGGGGGAAGCGCTCGTCCAGATTGGGCTGGGCGTCGGGGAGGATCACTTACTCTC
ACTCCCCGCCGCGGGGGTGGTGCTCAGCTGCTTGGCCGCCGCGACATGGCTGGTGCTGAGGCTGAGGCTG
GGCGTCCTCATGATCGCCTTGACTAGCGCGGTCAGGACCGTGTCCCTCATTTCCTTAGAGAGGTTCAAGG
TCGCCTGGAGACCTTACCTGGCGTACCTGGCCGGCGTGCTGGGGATCCTCTTGGCCAGGTACGTGGAACA
AATCTTGCCGCAGTCCGCGGAGGCGGCTCCAAGGGAGCATTTGGGGTCCCAGCTGATTGCTGGGACCAAG
GAAGATATCCCGGTGTTTAAGAGGAGGAGGCGGTCCAGCTCCGTCGTGTCCGCCGAGATGTCCGGCTGCA
GCAGCAAGTCCCATCGGAGGACCTCCCTGCCCTGTATACCGAGGGAACAGCTCATGGGGCATTCAGAATG
GGACCACAAACGAGGGCCAAGAGGATCACAGTCTTCAGGAACCAGTATTACTGTGGACATCGCCGTCATG
GGCGAGGCCCACGGCCTCATTACCGACCTCCTGGCAGACCCTTCTCTTCCACCAAACGTGTGCACATCCT
TGAGAGCCGTGAGCAACTTGCTCAGCACACAGCTCACCTTCCAGGCCATTCACAAGCCCAGAGTGAATCC
CGTTACTTCGCTCAGTGAAAACTATACCTGTTCTGACTCTGAAGAGAGCTCTGAAAAAGACAAGCTTGCT
ATTCCAAAGCGCCTGAGAAGGAGTTTGCCTCCTGGCTTGTTGAGACGAGTTTCTTCCACTTGGACCACCA
CCACCTCGGCCACAGGTCTACCCACCTTGGAGCCTGCACCAGTACGGAGAGACCGCAGCACCAGCATCAA
ACTGCAGGAAGCACCTTCATCCAGTCCTGATTCTTGGAATAATCCAGTGATGATGACCCTCACCAAAAGC
AGATCCTTTACTTCATCCTATGCTATTTCTGCAGCTAACCATGTAAAGGCTAAAAAGCAAAGTCGACCAG
GTGCCCTCGCTAAAATTTCACCTCTTTCATCGCCCTGCTCCTCACCTCTCCAAGGGACTCCTGCCAGCAG
CCTGGTCAGCAAAATTTCTGCAGTGCAGTTTCCAGAATCTGCTGACACAACTGCCAAACAAAGCCTAGGT
TCTCACAGGGCCTTAACTTACACTCAGAGTGCCCCAGACCTATCCCCTCAAATCCTGACTCCACCTGTTA
TATGTAGCAGCTGTGGCAGACCATATTCCCAAGGGAATCCTGCTGATGAGCCCCTGGAGAGAAGTGGGGT
AGCCACTCGGACACCAAGTAGAACAGATGACACTGCTCAAGTTACCTCTGATTATGAAACCAATAACAAC
AGTGACAGCAGTGACATTGTACAGAATGAAGATGAAACAGAGTGCCTGAGAGAGCCTCTGAGGAAAGCAT
CGGCTTGCAGCACCTATGCTCCTGAGACCATGATGTTTCTGGACAAACCAATTCTTGCTCCCGAACCTCT
TGTCATGGATAACCTGGACTCAATTATGGAGCAGCTAAATACTTGGAATTTTCCAATTTTTGATTTAGTG
GAAAATATAGGAAGAAAATGTGGCCGTATTCTTAGTCAGGTATCTTACAGACTTTTTGAAGACATGGGCC
TGTTTGAAGCTTTTAAAATTCCAATTAGGGAATTTATGAATTATTTTCATGCTTTGGAGATTGGATATAG
GGATATTCCTTATCATAACAGAATCCATGCCACTGATGTTTTACATGCTGTTTGGTATCTTACTACACAG
CCTATTCCAGGCCTCTCAACTGTGATTAATGATCATGGTTCAACCAGTGATTCAGATTCTGACAGTGGAT
TTACACATGGACATATGGGATATGTATTCTCAAAAACGTATAATGTGACAGATGATAAATACGGATGTCT
GTCTGGGAATATCCCTGCCTTGGAGTTGATGGCGCTGTATGTGGCTGCAGCCATGCACGATTATGATCAT
CCAGGAAGGACTAATGCTTTCCTGGTTGCAACTAGTGCTCCTCAGGCGGTGCTATATAACGATCGTTCAG
TTTTGGAGAATCATCACGCAGCTGCTGCATGGAATCTTTTCATGTCCCGGCCAGAGTATAACTTCTTAAT
TAACCTTGACCATGTGGAATTTAAGCATTTCCGTTTCCTTGTCATTGAAGCAATTTTGGCCACTGACCTG
AAGAAACACTTTGACTTCGTAGCCAAATTTAATGGCAAGGTAAATGATGATGTTGGAATAGATTGGACCA
ATGAAAATGATCGTCTACTGGTTTGTCAAATGTGTATAAAGTTGGCTGATATCAATGGTCCAGCTAAATG
TAAAGAACTCCATCTTCAGTGGACAGATGGTATTGTCAATGAATTTTATGAACAGGGTGATGAAGAGGCC
AGCCTTGGATTACCCATAAGCCCCTTCATGGATCGTTCTGCTCCTCAGCTGGCCAACCTTCAGGAATCCT
TCATCTCTCACATTGTGGGGCCTCTGTGCAACTCCTATGATTCAGCAGGACTAATGCCTGGAAAATGGGT
GGAAGACAGCGATGAGTCAGGAGATACTGATGACCCAGAAGAAGAGGAGGAAGAAGCACCAGCACCAAAT
GAAGAGGAAACCTGTGAAAATAATGAATCTCCAAAAAAGAAGACTTTCAAAAGGAGAAAAATCTACTGCC
AAATAACTCAGCACCTCTTACAGAACCACAAGATGTGGAAGAAAGTCATTGAAGAGGAGCAACGGTTGGC
AGGCATAGAAAATCAATCCCTGGACCAGACCCCTCAGTCGCACTCTTCAGAACAGATCCAGGCTATCAAG
GAAGAAGAAGAAGAGAAAGGGAAACCAAGAGGCGAGGAGATACCAACCCAAAAGCCAGACCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222006 representing NM_000921
Red=Cloning site Green=Tags(s)

MAVPGDAARVRDKPVHSGVSQAPTAGRDCHHRADPASPRDSGCRGCWGDLVLQPLRSSRKLSSALCAGSL
SFLLALLVRLVRGEVGCDLEQCKEAAAAEEEEAAPGAEGGVFPGPRGGAPGGGARLSPWLQPSALLFSLL
CAFFWMGLYLLRAGVRLPLAVALLAACCGGEALVQIGLGVGEDHLLSLPAAGVVLSCLAAATWLVLRLRL
GVLMIALTSAVRTVSLISLERFKVAWRPYLAYLAGVLGILLARYVEQILPQSAEAAPREHLGSQLIAGTK
EDIPVFKRRRRSSSVVSAEMSGCSSKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSSGTSITVDIAVM
GEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLA
IPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKS
RSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLG
SHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNN
SDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLV
ENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQ
PIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDH
PGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDL
KKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEA
SLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPN
EEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQSHSSEQIQAIK
EEEEEKGKPRGEEIPTQKPDQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000921
ORF Size 3423 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000921.5
RefSeq Size 4124 bp
RefSeq ORF 3426 bp
Locus ID 5139
UniProt ID Q14432
Cytogenetics 12p12.2
Protein Families Druggable Genome, Transmembrane
Protein Pathways Insulin signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
MW 124.8 kDa
Summary This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Sep 2011
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"NM_000921" in other vectors (6)
SKU Description Size Price
RC222006L1 Lenti ORF clone of Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), Myc-DDK-tagged 10 ug
$1,293.00
RC222006L2 Lenti ORF clone of Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), mGFP tagged 10 ug
$1,293.00
RC222006L3 Lenti ORF clone of Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), Myc-DDK-tagged 10 ug
$1,293.00
RC222006L4 Lenti ORF clone of Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), mGFP tagged 10 ug
$1,293.00
RG222006 PDE3A (tGFP-tagged) - Human phosphodiesterase 3A, cGMP-inhibited (PDE3A) 10 ug
$789.00 $1,193.00
SC300151 PDE3A (untagged)-Human phosphodiesterase 3A, cGMP-inhibited (PDE3A) 10 ug
$994.00

Plasmid Map for RC222006

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222006
Circular map for RC222006

file-alt Citations

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