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PDE3A (NM_000921) Human Untagged Clone

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SKU
SC300151
PDE3A (untagged)-Human phosphodiesterase 3A, cGMP-inhibited (PDE3A)
  $994.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PDE3A
Synonyms CGI-PDE; CGI-PDE-A; CGI-PDE A; HTNB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_000921 edited
GGGGGCCACTGGGAATTCAGTGAAGAGGGCACCCTATACCATGGCAGTGCCCGGCGACGC
TGCACGAGTCAGGGACAAGCCCGTCCACAGTGGGGTGAGTCAAGCCCCCACGGCGGGCCG
GGACTGCCACCATCGTGCGGACCCCGCATCGCCGCGGGACTCGGGCTGCCGTGGCTGCTG
GGGAGACCTGGTGCTGCAGCCGCTCCGGAGCTCTCGGAAACTTTCCTCCGCGCTGTGCGC
GGGCTCCCTGTCCTTTCTGCTGGCGCTGCTGGTGAGGCTGGTCCGCGGGGAGGTCGGCTG
TGACCTGGAGCAGTGTAAGGAGGCGGCGGCGGCGGAGGAGGAGGAAGCAGCCCCGGGAGC
AGAAGGGGGCGTCTTCCCGGGGCCTCGGGGAGGTGCTCCCGGGGGCGGTGCGCGGCTCAG
CCCCTGGCTGCAGCCCTCGGCGCTGCTCTTCAGTCTCCTGTGTGCCTTCTTCTGGATGGG
CTTGTACCTCCTGCGCGCCGGGGTGCGCCTGCCTCTGGCTGTCGCGCTGCTGGCCGCCTG
CTGCGGGGGGGAAGCGCTCGTCCAGATTGGGCTGGGCGTCGGGGAGGATCACTTACTCTC
ACTCCCCGCCGCGGGGGTGGTGCTCAGCTGCTTGGCCGCCGCGACATGGCTGGTGCTGAG
GCTGAGGCTGGGCGTCCTCATGATCGCCTTGACTAGCGCGGTCAGGACCGTGTCCCTCAT
TTCCTTAGAGAGGTTCAAGGTCGCCTGGAGACCTTACCTGGCGTACCTGGCCGGCGTGCT
GGGGATCCTCTTGGCCAGGTACGTGGAACAAATCTTGCCGCAGTCCGCGGAGGCGGCTCC
AAGGGAGCATTTGGGGTCCCAGCTGATTGCTGGGACCAAGGAAGATATCCCGGTGTTTAA
GAGGAGGAGGCGGTCCAGCTCCGTCGTGTCCGCCGAGATGTCCGGCTGCAGCAGCAAGTC
CCATCGGAGGACCTCCCTGCCCTGTATACCGAGGGAACAGCTCATGGGGCATTCAGAATG
GGACCACAAACGAGGGCCAAGAGGATCACAGTCTTCAGGAACCAGTATTACTGTGGACAT
CGCCGTCATGGGCGAGGCCCACGGCCTCATTACCGACCTCCTGGCAGACCCTTCTCTTCC
ACCAAACGTGTGCACATCCTTGAGAGCCGTGAGCAACTTGCTCAGCACACAGCTCACCTT
CCAGGCCATTCACAAGCCCAGAGTGAATCCCGTTACTTCGCTCAGTGAAAACTATACCTG
TTCTGACTCTGAAGAGAGCTCTGAAAAAGACAAGCTTGCTATTCCAAAGCGCCTGAGAAG
GAGTTTGCCTCCTGGCTTGTTGAGACGAGTTTCTTCCACTTGGACCACCACCACCTCGGC
CACAGGTCTACCCACCTTGGAGCCTGCACCAGTACGGAGAGACCGCAGCACCAGCATCAA
ACTGCAGGAAGCACCTTCATCCAGTCCTGATTCTTGGAATAATCCAGTGATGATGACCCT
CACCAAAAGCAGATCCTTTACTTCATCCTATGCTATTTCTGCAGCTAACCATGTAAAGGC
TAAAAAGCAAAGTCGACCAGGTGCCCTCGCTAAAATTTCACCTCTTTCATCGCCCTGCTC
CTCACCTCTCCAAGGGACTCCTGCCAGCAGCCTGGTCAGCAAAATTTCTGCAGTGCAGTT
TCCAGAATCTGCTGACACAACTGCCAAACAAAGCCTAGGTTCTCACAGGGCCTTAACTTA
CACTCAGAGTGCCCCAGACCTATCCCCTCAAATCCTGACTCCACCTGTTATATGTAGCAG
CTGTGGCAGACCATATTCCCAAGGGAATCCTGCTGATGAGCCCCTGGAGAGAAGTGGGGT
AGCCACTCGGACACCAAGTAGAACAGATGACACTGCTCAAGTTACCTCTGATTATGAAAC
CAATAACAACAGTGACAGCAGTGACATTGTACAGAATGAAGATGAAACAGAGTGCCTGAG
AGAGCCTCTGAGGAAAGCATCGGCTTGCAGCACCTATGCTCCTGAGACCATGATGTTTCT
GGACAAACCAATTCTTGCTCCCGAACCTCTTGTCATGGATAACCTGGACTCAATTATGGA
GCAGCTAAATACTTGGAATTTTCCAATTTTTGATTTAGTGGAAAATATAGGAAGAAAATG
TGGCCGTATTCTTAGTCAGGTATCTTACAGACTTTTTGAAGACATGGGCCTGTTTGAAGC
TTTTAAAATTCCAATTAGGGAATTTATGAATTATTTTCATGCTTTGGAGATTGGATATAG
GGATATTCCTTATCATAACAGAATCCATGCCACTGATGTTTTACATGCTGTTTGGTATCT
TACTACACAGCCTATTCCAGGCCTCTCAACTGTGATTAATGATCATGGTTCAACCAGTGA
TTCAGATTCTGACAGTGGATTTACACATGGACATATGGGATATGTATTCTCAAAAACGTA
TAATGTGACAGATGATAAATACGGATGTCTGTCTGGGAATATCCCTGCCTTGGAGTTGAT
GGCGCTGTATGTGGCTGCAGCCATGCACGATTATGATCATCCAGGAAGGACTAATGCTTT
CCTGGTTGCAACTAGTGCTCCTCAGGCGGTGCTATATAACGATCGTTCAGTTTTGGAGAA
TCATCACGCAGCTGCTGCATGGAATCTTTTCATGTCCCGGCCAGAGTATAACTTCTTAAT
TAACCTTGACCATGTGGAATTTAAGCATTTCCGTTTCCTTGTCATTGAAGCAATTTTGGC
CACTGACCTGAAGAAACACTTTGACTTCGTAGCCAAATTTAATGGCAAGGTAAATGATGA
TGTTGGAATAGATTGGACCAATGAAAATGATCGTCTACTGGTTTGTCAAATGTGTATAAA
GTTGGCTGATATCAATGGTCCAGCTAAATGTAAAGAACTCCATCTTCAGTGGACAGATGG
TATTGTCAATGAATTTTATGAACAGGGTGATGAAGAGGCCAGCCTTGGATTACCCATAAG
CCCCTTCATGGATCGTTCTGCTCCTCAGCTGGCCAACCTTCAGGAATCCTTCATCTCTCA
CATTGTGGGGCCTCTGTGCAACTCCTATGATTCAGCAGGACTAATGCCTGGAAAATGGGT
GGAAGACAGCGATGAGTCAGGAGATACTGATGACCCAGAAGAAGAGGAGGAAGAAGCACC
AGCACCAAATGAAGAGGAAACCTGTGAAAATAATGAATCTCCAAAAAAGAAGACTTTCAA
AAGGAGAAAAATCTACTGCCAAATAACTCAGCACCTCTTACAGAACCACAAGATGTGGAA
GAAAGTCATTGAAGAGGAGCAACGGTTGGCAGGCATAGAAAATCAATCCCTGGACCAGAC
CCCTCAGTCGCACTCTTCAGAACAGATCCAGGCTATCAAGGAAGAAGAAGAAGAGAAAGG
GAAACCAAGAGGCGAGGAGATACCAACCCAAAAGCCAGACCAGTGACAATGGATAGAATG
GGCTGTGTTTCCAAACAGATTGACTTGTCAAAGACTCTCTTCAAGCCAGCACAAGCA
Restriction Sites Please inquire |
ACCN NM_000921
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000921.3, NP_000912.3
RefSeq Size 4124 bp
RefSeq ORF 3426 bp
Locus ID 5139
UniProt ID Q14432
Cytogenetics 12p12.2
Protein Families Druggable Genome, Transmembrane
Protein Pathways Insulin signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
Summary This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Sep 2011
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_000921" in other vectors (6)
SKU Description Size Price
RC222006 PDE3A (Myc-DDK-tagged)-Human phosphodiesterase 3A, cGMP-inhibited (PDE3A) 10 ug
$993.00
RC222006L1 Lenti ORF clone of Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), Myc-DDK-tagged 10 ug
$1,293.00
RC222006L2 Lenti ORF clone of Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), mGFP tagged 10 ug
$1,293.00
RC222006L3 Lenti ORF clone of Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), Myc-DDK-tagged 10 ug
$1,293.00
RC222006L4 Lenti ORF clone of Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), mGFP tagged 10 ug
$1,293.00
RG222006 PDE3A (tGFP-tagged) - Human phosphodiesterase 3A, cGMP-inhibited (PDE3A) 10 ug
$789.00 $1,193.00

file-alt Citations

Clinical Effects of Phosphodiesterase 3A Mutations in Inherited Hypertension With Brachydactyly
Toka, Tank, Schächterle et al
Hypertension (2015)
Cited by 1
AI Summary: In this study, OriGene’s full-length wild-type PDE3A cDNA vector (SC300151) and empty vector (sc300-w/o) were used to transiently transfect HeLa cells, enabling functional analysis of PDE3A mutations relevant to inherited hypertension with brachydactyly.
Snippet: Platelets, and Human Heart Lysates A commonly used, cancer-derived cell line named after Henrietta Lacks (HeLa) cells were transiently transfected with an empty vector (sc300-w/o) or a vector encoding full-length wild-type PDE3A cDNA (Origene, SC300151, ... Cite SaveSaving
PDE3A mutations cause autosomal dominant hypertension with brachydactyly
Maass, Aydin, Luft et al
Nat Genet (2015) 47 (6), 647-53 DOI: 10.1038/ng.3302
Cited by 171
AI Summary: OriGene products, specifically the PDE3A cDNA clone (RC208889) and PDE3A shRNA (TL313482), were used in this study to investigate the functional effects of PDE3A mutations by enabling overexpression and knockdown experiments in cell models.
Showing 1-2 of 2 papers.
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